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Metabolic Engineering of Microalgal Based Biofuel Production: Prospects and Challenges

The current scenario in renewable energy is focused on development of alternate and sustainable energy sources, amongst which microalgae stands as one of the promising feedstock for biofuel production. It is well known that microalgae generate much larger amounts of biofuels in a shorter time than o...

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Autores principales: Banerjee, Chiranjib, Dubey, Kashyap K., Shukla, Pratyoosh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4815533/
https://www.ncbi.nlm.nih.gov/pubmed/27065986
http://dx.doi.org/10.3389/fmicb.2016.00432
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author Banerjee, Chiranjib
Dubey, Kashyap K.
Shukla, Pratyoosh
author_facet Banerjee, Chiranjib
Dubey, Kashyap K.
Shukla, Pratyoosh
author_sort Banerjee, Chiranjib
collection PubMed
description The current scenario in renewable energy is focused on development of alternate and sustainable energy sources, amongst which microalgae stands as one of the promising feedstock for biofuel production. It is well known that microalgae generate much larger amounts of biofuels in a shorter time than other sources based on plant seeds. However, the greatest challenge in a transition to algae-based biofuel production is the various other complications involved in microalgal cultivation, its harvesting, concentration, drying and lipid extraction. Several green microalgae accumulate lipids, especially triacylglycerols (TAGs), which are main precursors in the production of lipid. The various aspects on metabolic pathway analysis of an oleaginous microalgae i.e., Chlamydomonas reinhardtii have elucidated some novel metabolically important genes and this enhances the lipid production in this microalgae. Adding to it, various other aspects in metabolic engineering using OptFlux and effectual bioprocess design also gives an interactive snapshot of enhancing lipid production which ultimately improvises the oil yield. This article reviews the current status of microalgal based technologies for biofuel production, bioreactor process design, flux analysis and it also provides various strategies to increase lipids accumulation via metabolic engineering.
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spelling pubmed-48155332016-04-08 Metabolic Engineering of Microalgal Based Biofuel Production: Prospects and Challenges Banerjee, Chiranjib Dubey, Kashyap K. Shukla, Pratyoosh Front Microbiol Microbiology The current scenario in renewable energy is focused on development of alternate and sustainable energy sources, amongst which microalgae stands as one of the promising feedstock for biofuel production. It is well known that microalgae generate much larger amounts of biofuels in a shorter time than other sources based on plant seeds. However, the greatest challenge in a transition to algae-based biofuel production is the various other complications involved in microalgal cultivation, its harvesting, concentration, drying and lipid extraction. Several green microalgae accumulate lipids, especially triacylglycerols (TAGs), which are main precursors in the production of lipid. The various aspects on metabolic pathway analysis of an oleaginous microalgae i.e., Chlamydomonas reinhardtii have elucidated some novel metabolically important genes and this enhances the lipid production in this microalgae. Adding to it, various other aspects in metabolic engineering using OptFlux and effectual bioprocess design also gives an interactive snapshot of enhancing lipid production which ultimately improvises the oil yield. This article reviews the current status of microalgal based technologies for biofuel production, bioreactor process design, flux analysis and it also provides various strategies to increase lipids accumulation via metabolic engineering. Frontiers Media S.A. 2016-03-31 /pmc/articles/PMC4815533/ /pubmed/27065986 http://dx.doi.org/10.3389/fmicb.2016.00432 Text en Copyright © 2016 Banerjee, Dubey and Shukla. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Banerjee, Chiranjib
Dubey, Kashyap K.
Shukla, Pratyoosh
Metabolic Engineering of Microalgal Based Biofuel Production: Prospects and Challenges
title Metabolic Engineering of Microalgal Based Biofuel Production: Prospects and Challenges
title_full Metabolic Engineering of Microalgal Based Biofuel Production: Prospects and Challenges
title_fullStr Metabolic Engineering of Microalgal Based Biofuel Production: Prospects and Challenges
title_full_unstemmed Metabolic Engineering of Microalgal Based Biofuel Production: Prospects and Challenges
title_short Metabolic Engineering of Microalgal Based Biofuel Production: Prospects and Challenges
title_sort metabolic engineering of microalgal based biofuel production: prospects and challenges
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4815533/
https://www.ncbi.nlm.nih.gov/pubmed/27065986
http://dx.doi.org/10.3389/fmicb.2016.00432
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