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P4H9-detected molecule expression on spindle-shaped fibroblasts indicates malignant phenotype of colorectal cancer

BACKGROUND: Our previous study using a mammary fat pad mouse model showed that P4H9, produced by the β2 integrin epitope, detected a molecule on fibroblasts in response to carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1)-expressing cancer cells. P4H9-detected molecule (PDM) expres...

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Autores principales: Yokoyama, Shozo, Ieda, Junji, Yamamoto, Naoyuki, Yamaguchi, Shunsuke, Mitani, Yasuyuki, Takeuchi, Akihiro, Takifuji, Katsunari, Hotta, Tsukasa, Matsuda, Kenji, Watanabe, Takashi, Shively, John E, Yamaue, Hiroki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4815886/
https://www.ncbi.nlm.nih.gov/pubmed/26469833
http://dx.doi.org/10.1038/bjc.2015.363
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author Yokoyama, Shozo
Ieda, Junji
Yamamoto, Naoyuki
Yamaguchi, Shunsuke
Mitani, Yasuyuki
Takeuchi, Akihiro
Takifuji, Katsunari
Hotta, Tsukasa
Matsuda, Kenji
Watanabe, Takashi
Shively, John E
Yamaue, Hiroki
author_facet Yokoyama, Shozo
Ieda, Junji
Yamamoto, Naoyuki
Yamaguchi, Shunsuke
Mitani, Yasuyuki
Takeuchi, Akihiro
Takifuji, Katsunari
Hotta, Tsukasa
Matsuda, Kenji
Watanabe, Takashi
Shively, John E
Yamaue, Hiroki
author_sort Yokoyama, Shozo
collection PubMed
description BACKGROUND: Our previous study using a mammary fat pad mouse model showed that P4H9, produced by the β2 integrin epitope, detected a molecule on fibroblasts in response to carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1)-expressing cancer cells. P4H9-detected molecule (PDM) expression appeared to be associated with myofibroblast differentiation. In this study, we investigated whether PDM is expressed on fibroblasts and cancer cells in clinical tissue samples, and whether the presence of PDM-expressing colorectal cancer cells is correlated with clinicopathological features of patients. METHODS: Immunohistochemistry was conducted to detect P4H9 on clinical tissue samples from 156 patients with colorectal cancer. Risk factors for metastases and survival were calculated for clinical implication of PDM-expressing spindle-shaped fibroblasts. RESULTS: Multivariate analysis showed that PDM-expressing spindle-shaped fibroblasts were an independent risk factor for lymph node metastasis, hematogenous metastasis, and poor survival. A Kaplan–Meier survival curve indicated that PDM-expressing spindle-shaped fibroblasts were associated with shorter survival time (P<0.0001). Immunofluorescence showed PDM expression on CCD-18Co fibroblasts and two colorectal cancer cell lines (HCT116 and HCT-15). CONCLUSIONS: PDM-expressing spindle-shaped fibroblasts are associated with metastasis and shorter survival in colorectal cancer patients. PDM-expressing spindle-shaped fibroblasts may have a role in eliciting the malignant phenotype of colorectal cancer.
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spelling pubmed-48158862016-11-17 P4H9-detected molecule expression on spindle-shaped fibroblasts indicates malignant phenotype of colorectal cancer Yokoyama, Shozo Ieda, Junji Yamamoto, Naoyuki Yamaguchi, Shunsuke Mitani, Yasuyuki Takeuchi, Akihiro Takifuji, Katsunari Hotta, Tsukasa Matsuda, Kenji Watanabe, Takashi Shively, John E Yamaue, Hiroki Br J Cancer Molecular Diagnostics BACKGROUND: Our previous study using a mammary fat pad mouse model showed that P4H9, produced by the β2 integrin epitope, detected a molecule on fibroblasts in response to carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1)-expressing cancer cells. P4H9-detected molecule (PDM) expression appeared to be associated with myofibroblast differentiation. In this study, we investigated whether PDM is expressed on fibroblasts and cancer cells in clinical tissue samples, and whether the presence of PDM-expressing colorectal cancer cells is correlated with clinicopathological features of patients. METHODS: Immunohistochemistry was conducted to detect P4H9 on clinical tissue samples from 156 patients with colorectal cancer. Risk factors for metastases and survival were calculated for clinical implication of PDM-expressing spindle-shaped fibroblasts. RESULTS: Multivariate analysis showed that PDM-expressing spindle-shaped fibroblasts were an independent risk factor for lymph node metastasis, hematogenous metastasis, and poor survival. A Kaplan–Meier survival curve indicated that PDM-expressing spindle-shaped fibroblasts were associated with shorter survival time (P<0.0001). Immunofluorescence showed PDM expression on CCD-18Co fibroblasts and two colorectal cancer cell lines (HCT116 and HCT-15). CONCLUSIONS: PDM-expressing spindle-shaped fibroblasts are associated with metastasis and shorter survival in colorectal cancer patients. PDM-expressing spindle-shaped fibroblasts may have a role in eliciting the malignant phenotype of colorectal cancer. Nature Publishing Group 2015-11-17 2015-10-15 /pmc/articles/PMC4815886/ /pubmed/26469833 http://dx.doi.org/10.1038/bjc.2015.363 Text en Copyright © 2015 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/4.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Molecular Diagnostics
Yokoyama, Shozo
Ieda, Junji
Yamamoto, Naoyuki
Yamaguchi, Shunsuke
Mitani, Yasuyuki
Takeuchi, Akihiro
Takifuji, Katsunari
Hotta, Tsukasa
Matsuda, Kenji
Watanabe, Takashi
Shively, John E
Yamaue, Hiroki
P4H9-detected molecule expression on spindle-shaped fibroblasts indicates malignant phenotype of colorectal cancer
title P4H9-detected molecule expression on spindle-shaped fibroblasts indicates malignant phenotype of colorectal cancer
title_full P4H9-detected molecule expression on spindle-shaped fibroblasts indicates malignant phenotype of colorectal cancer
title_fullStr P4H9-detected molecule expression on spindle-shaped fibroblasts indicates malignant phenotype of colorectal cancer
title_full_unstemmed P4H9-detected molecule expression on spindle-shaped fibroblasts indicates malignant phenotype of colorectal cancer
title_short P4H9-detected molecule expression on spindle-shaped fibroblasts indicates malignant phenotype of colorectal cancer
title_sort p4h9-detected molecule expression on spindle-shaped fibroblasts indicates malignant phenotype of colorectal cancer
topic Molecular Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4815886/
https://www.ncbi.nlm.nih.gov/pubmed/26469833
http://dx.doi.org/10.1038/bjc.2015.363
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