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QTL Mapping of Low-Temperature Germination Ability in the Maize IBM Syn4 RIL Population

Low temperature is the primary factor to affect maize sowing in early spring. It is, therefore, vital for maize breeding programs to improve tolerance to low temperatures at seed germination stage. However, little is known about maize QTL involved in low-temperature germination ability. 243 lines of...

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Autores principales: Hu, Shuaidong, Lübberstedt, Thomas, Zhao, Guangwu, Lee, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816396/
https://www.ncbi.nlm.nih.gov/pubmed/27031623
http://dx.doi.org/10.1371/journal.pone.0152795
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author Hu, Shuaidong
Lübberstedt, Thomas
Zhao, Guangwu
Lee, Michael
author_facet Hu, Shuaidong
Lübberstedt, Thomas
Zhao, Guangwu
Lee, Michael
author_sort Hu, Shuaidong
collection PubMed
description Low temperature is the primary factor to affect maize sowing in early spring. It is, therefore, vital for maize breeding programs to improve tolerance to low temperatures at seed germination stage. However, little is known about maize QTL involved in low-temperature germination ability. 243 lines of the intermated B73×Mo17 (IBM) Syn4 recombinant inbred line (RIL) population was used for QTL analysis of low-temperature germination ability. There were significant differences in germination-related traits under both conditions of low temperature (12°C/16h, 18°C/8h) and optimum temperature (28°C/24h) between the parental lines. Only three QTL were identified for controlling optimum-temperature germination rate. Six QTL controlling low-temperature germination rate were detected on chromosome 4, 5, 6, 7 and 9, and contribution rate of single QTL explained between 3.39%~11.29%. In addition, six QTL controlling low-temperature primary root length were detected in chromosome 4, 5, 6, and 9, and the contribution rate of single QTL explained between 3.96%~8.41%. Four pairs of QTL were located at the same chromosome position and together controlled germination rate and primary root length under low temperature condition. The nearest markers apart from the corresponding QTL (only 0.01 cM) were umc1303 (265.1 cM) on chromosome 4, umc1 (246.4 cM) on chromosome 5, umc62 (459.1 cM) on chromosome 6, bnl14.28a (477.4 cM) on chromosome 9, respectively. A total of 3155 candidate genes were extracted from nine separate intervals based on the Maize Genetics and Genomics Database (http://www.maizegdb.org). Five candidate genes were selected for analysis as candidates putatively affecting seed germination and seedling growth at low temperature. The results provided a basis for further fine mapping, molecular marker assisted breeding and functional study of cold-tolerance at the stage of seed germination in maize.
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spelling pubmed-48163962016-04-14 QTL Mapping of Low-Temperature Germination Ability in the Maize IBM Syn4 RIL Population Hu, Shuaidong Lübberstedt, Thomas Zhao, Guangwu Lee, Michael PLoS One Research Article Low temperature is the primary factor to affect maize sowing in early spring. It is, therefore, vital for maize breeding programs to improve tolerance to low temperatures at seed germination stage. However, little is known about maize QTL involved in low-temperature germination ability. 243 lines of the intermated B73×Mo17 (IBM) Syn4 recombinant inbred line (RIL) population was used for QTL analysis of low-temperature germination ability. There were significant differences in germination-related traits under both conditions of low temperature (12°C/16h, 18°C/8h) and optimum temperature (28°C/24h) between the parental lines. Only three QTL were identified for controlling optimum-temperature germination rate. Six QTL controlling low-temperature germination rate were detected on chromosome 4, 5, 6, 7 and 9, and contribution rate of single QTL explained between 3.39%~11.29%. In addition, six QTL controlling low-temperature primary root length were detected in chromosome 4, 5, 6, and 9, and the contribution rate of single QTL explained between 3.96%~8.41%. Four pairs of QTL were located at the same chromosome position and together controlled germination rate and primary root length under low temperature condition. The nearest markers apart from the corresponding QTL (only 0.01 cM) were umc1303 (265.1 cM) on chromosome 4, umc1 (246.4 cM) on chromosome 5, umc62 (459.1 cM) on chromosome 6, bnl14.28a (477.4 cM) on chromosome 9, respectively. A total of 3155 candidate genes were extracted from nine separate intervals based on the Maize Genetics and Genomics Database (http://www.maizegdb.org). Five candidate genes were selected for analysis as candidates putatively affecting seed germination and seedling growth at low temperature. The results provided a basis for further fine mapping, molecular marker assisted breeding and functional study of cold-tolerance at the stage of seed germination in maize. Public Library of Science 2016-03-31 /pmc/articles/PMC4816396/ /pubmed/27031623 http://dx.doi.org/10.1371/journal.pone.0152795 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Hu, Shuaidong
Lübberstedt, Thomas
Zhao, Guangwu
Lee, Michael
QTL Mapping of Low-Temperature Germination Ability in the Maize IBM Syn4 RIL Population
title QTL Mapping of Low-Temperature Germination Ability in the Maize IBM Syn4 RIL Population
title_full QTL Mapping of Low-Temperature Germination Ability in the Maize IBM Syn4 RIL Population
title_fullStr QTL Mapping of Low-Temperature Germination Ability in the Maize IBM Syn4 RIL Population
title_full_unstemmed QTL Mapping of Low-Temperature Germination Ability in the Maize IBM Syn4 RIL Population
title_short QTL Mapping of Low-Temperature Germination Ability in the Maize IBM Syn4 RIL Population
title_sort qtl mapping of low-temperature germination ability in the maize ibm syn4 ril population
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816396/
https://www.ncbi.nlm.nih.gov/pubmed/27031623
http://dx.doi.org/10.1371/journal.pone.0152795
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