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Anti-Insulin Immune Responses Are Detectable in Dogs with Spontaneous Diabetes
Diabetes mellitus occurs spontaneously in dogs. Although canine diabetes shares many features with human type-1 diabetes, there are differences that have cast doubt on the immunologic origin of the canine disease. In this study, we examined whether peripheral immune responses directed against islet...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816536/ https://www.ncbi.nlm.nih.gov/pubmed/27031512 http://dx.doi.org/10.1371/journal.pone.0152397 |
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author | Kim, Jong-Hyuk Furrow, Eva Ritt, Michelle G. Utz, Paul J. Robinson, William H. Yu, Liping Eckert, Andrea Stuebner, Kathleen O’Brien, Timothy D. Steinman, Lawrence Modiano, Jaime F. |
author_facet | Kim, Jong-Hyuk Furrow, Eva Ritt, Michelle G. Utz, Paul J. Robinson, William H. Yu, Liping Eckert, Andrea Stuebner, Kathleen O’Brien, Timothy D. Steinman, Lawrence Modiano, Jaime F. |
author_sort | Kim, Jong-Hyuk |
collection | PubMed |
description | Diabetes mellitus occurs spontaneously in dogs. Although canine diabetes shares many features with human type-1 diabetes, there are differences that have cast doubt on the immunologic origin of the canine disease. In this study, we examined whether peripheral immune responses directed against islet antigens were present in dogs with diabetes. Routine diagnostics were used to confirm diabetic status, and serum samples from dogs with (N = 15) and without (N = 15) diabetes were analyzed for the presence of antibodies against islet antigens (insulin, glutamic acid decarboxylase, insulinoma-associated protein tyrosine phosphatase, and islet beta-cell zinc cation efflux transporter) using standard radioassays. Interferon-γ production from peripheral blood T cells stimulated by porcine insulin and by human insulin was tested using Elispot assays. Anti-insulin antibodies were detectable in a subset of diabetic dogs receiving insulin therapy. Pre-activated T cells and incipient insulin-reactive T cells in response to porcine or human insulin were identified in non-diabetic dogs and in dogs with diabetes. The data show that humoral and cellular anti-insulin immune responses are detectable in dogs with diabetes. This in turn provides support for the potential to ethically use dogs with diabetes to study the therapeutic potential of antigen-specific tolerance. |
format | Online Article Text |
id | pubmed-4816536 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48165362016-04-14 Anti-Insulin Immune Responses Are Detectable in Dogs with Spontaneous Diabetes Kim, Jong-Hyuk Furrow, Eva Ritt, Michelle G. Utz, Paul J. Robinson, William H. Yu, Liping Eckert, Andrea Stuebner, Kathleen O’Brien, Timothy D. Steinman, Lawrence Modiano, Jaime F. PLoS One Research Article Diabetes mellitus occurs spontaneously in dogs. Although canine diabetes shares many features with human type-1 diabetes, there are differences that have cast doubt on the immunologic origin of the canine disease. In this study, we examined whether peripheral immune responses directed against islet antigens were present in dogs with diabetes. Routine diagnostics were used to confirm diabetic status, and serum samples from dogs with (N = 15) and without (N = 15) diabetes were analyzed for the presence of antibodies against islet antigens (insulin, glutamic acid decarboxylase, insulinoma-associated protein tyrosine phosphatase, and islet beta-cell zinc cation efflux transporter) using standard radioassays. Interferon-γ production from peripheral blood T cells stimulated by porcine insulin and by human insulin was tested using Elispot assays. Anti-insulin antibodies were detectable in a subset of diabetic dogs receiving insulin therapy. Pre-activated T cells and incipient insulin-reactive T cells in response to porcine or human insulin were identified in non-diabetic dogs and in dogs with diabetes. The data show that humoral and cellular anti-insulin immune responses are detectable in dogs with diabetes. This in turn provides support for the potential to ethically use dogs with diabetes to study the therapeutic potential of antigen-specific tolerance. Public Library of Science 2016-03-31 /pmc/articles/PMC4816536/ /pubmed/27031512 http://dx.doi.org/10.1371/journal.pone.0152397 Text en © 2016 Kim et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kim, Jong-Hyuk Furrow, Eva Ritt, Michelle G. Utz, Paul J. Robinson, William H. Yu, Liping Eckert, Andrea Stuebner, Kathleen O’Brien, Timothy D. Steinman, Lawrence Modiano, Jaime F. Anti-Insulin Immune Responses Are Detectable in Dogs with Spontaneous Diabetes |
title | Anti-Insulin Immune Responses Are Detectable in Dogs with Spontaneous Diabetes |
title_full | Anti-Insulin Immune Responses Are Detectable in Dogs with Spontaneous Diabetes |
title_fullStr | Anti-Insulin Immune Responses Are Detectable in Dogs with Spontaneous Diabetes |
title_full_unstemmed | Anti-Insulin Immune Responses Are Detectable in Dogs with Spontaneous Diabetes |
title_short | Anti-Insulin Immune Responses Are Detectable in Dogs with Spontaneous Diabetes |
title_sort | anti-insulin immune responses are detectable in dogs with spontaneous diabetes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816536/ https://www.ncbi.nlm.nih.gov/pubmed/27031512 http://dx.doi.org/10.1371/journal.pone.0152397 |
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