Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents

SHuffle strains are genetically engineered Escherichia coli strains that are capable of oxidizing cysteines within proteins to form disulfide bonds. Here we present the complete genome of both the K-12 and B versions of SHuffle strains along with their parental ancestors. These strains have been of...

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Detalles Bibliográficos
Autores principales: Anton, Brian P., Fomenkov, Alexey, Raleigh, Elisabeth A., Berkmen, Mehmet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Prokaryotes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816632/
https://www.ncbi.nlm.nih.gov/pubmed/27034504
http://dx.doi.org/10.1128/genomeA.00230-16
Descripción
Sumario:SHuffle strains are genetically engineered Escherichia coli strains that are capable of oxidizing cysteines within proteins to form disulfide bonds. Here we present the complete genome of both the K-12 and B versions of SHuffle strains along with their parental ancestors. These strains have been of significant use to both the general scientific community and the biotech industry, interested in producing novel disulfide-bonded proteins that were hitherto unable to be expressed in standard E. coli expression strains.

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