Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents

SHuffle strains are genetically engineered Escherichia coli strains that are capable of oxidizing cysteines within proteins to form disulfide bonds. Here we present the complete genome of both the K-12 and B versions of SHuffle strains along with their parental ancestors. These strains have been of...

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Detalles Bibliográficos
Autores principales: Anton, Brian P., Fomenkov, Alexey, Raleigh, Elisabeth A., Berkmen, Mehmet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Prokaryotes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816632/
https://www.ncbi.nlm.nih.gov/pubmed/27034504
http://dx.doi.org/10.1128/genomeA.00230-16
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author Anton, Brian P.
Fomenkov, Alexey
Raleigh, Elisabeth A.
Berkmen, Mehmet
author_facet Anton, Brian P.
Fomenkov, Alexey
Raleigh, Elisabeth A.
Berkmen, Mehmet
author_sort Anton, Brian P.
collection PubMed
description SHuffle strains are genetically engineered Escherichia coli strains that are capable of oxidizing cysteines within proteins to form disulfide bonds. Here we present the complete genome of both the K-12 and B versions of SHuffle strains along with their parental ancestors. These strains have been of significant use to both the general scientific community and the biotech industry, interested in producing novel disulfide-bonded proteins that were hitherto unable to be expressed in standard E. coli expression strains.
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spelling pubmed-48166322016-04-04 Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents Anton, Brian P. Fomenkov, Alexey Raleigh, Elisabeth A. Berkmen, Mehmet Genome Announc Prokaryotes SHuffle strains are genetically engineered Escherichia coli strains that are capable of oxidizing cysteines within proteins to form disulfide bonds. Here we present the complete genome of both the K-12 and B versions of SHuffle strains along with their parental ancestors. These strains have been of significant use to both the general scientific community and the biotech industry, interested in producing novel disulfide-bonded proteins that were hitherto unable to be expressed in standard E. coli expression strains. American Society for Microbiology 2016-03-31 /pmc/articles/PMC4816632/ /pubmed/27034504 http://dx.doi.org/10.1128/genomeA.00230-16 Text en Copyright © 2016 Anton et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Prokaryotes
Anton, Brian P.
Fomenkov, Alexey
Raleigh, Elisabeth A.
Berkmen, Mehmet
Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents
title Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents
title_full Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents
title_fullStr Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents
title_full_unstemmed Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents
title_short Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents
title_sort complete genome sequence of the engineered escherichia coli shuffle strains and their wild-type parents
topic Prokaryotes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816632/
https://www.ncbi.nlm.nih.gov/pubmed/27034504
http://dx.doi.org/10.1128/genomeA.00230-16
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