Cargando…
Transfer of Viral Communities between Human Individuals during Fecal Microbiota Transplantation
Fecal microbiota transplantation (FMT) is a highly effective treatment for refractory Clostridium difficile infections. However, concerns persist about unwanted cotransfer of pathogenic microbes such as viruses. Here we studed FMT from a single healthy human donor to three pediatric ulcerative colit...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Microbiology
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4817255/ https://www.ncbi.nlm.nih.gov/pubmed/27025251 http://dx.doi.org/10.1128/mBio.00322-16 |
_version_ | 1782424863071272960 |
---|---|
author | Chehoud, Christel Dryga, Anatoly Hwang, Young Nagy-Szakal, Dorottya Hollister, Emily B. Luna, Ruth Ann Versalovic, James Kellermayer, Richard Bushman, Frederic D. |
author_facet | Chehoud, Christel Dryga, Anatoly Hwang, Young Nagy-Szakal, Dorottya Hollister, Emily B. Luna, Ruth Ann Versalovic, James Kellermayer, Richard Bushman, Frederic D. |
author_sort | Chehoud, Christel |
collection | PubMed |
description | Fecal microbiota transplantation (FMT) is a highly effective treatment for refractory Clostridium difficile infections. However, concerns persist about unwanted cotransfer of pathogenic microbes such as viruses. Here we studed FMT from a single healthy human donor to three pediatric ulcerative colitis patients, each of whom received a course of 22 to 30 FMT treatments. Viral particles were purified from donor and recipient stool samples and sequenced; the reads were then assembled into contigs corresponding to viral genomes or partial genomes. Transfer of selected viruses was confirmed by quantitative PCR. Viral contigs present in the donor could be readily detected in recipients, with up to 32 different donor viral contigs appearing in a recipient sample. Reassuringly, none of these were viruses are known to replicate on human cells. Instead, viral contigs either scored as bacteriophage or could not be attributed taxonomically, suggestive of unstudied phage. The two most frequently transferred gene types were associated with temperate-phage replication. In addition, members of Siphoviridae, the group of typically temperate phages that includes phage lambda, were found to be transferred with significantly greater efficiency than other groups. On the basis of these findings, we propose that the temperate-phage replication style may promote efficient phage transfer between human individuals. In summary, we documented transfer of multiple viral lineages between human individuals through FMT, but in this case series, none were from viral groups known to infect human cells. |
format | Online Article Text |
id | pubmed-4817255 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | American Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-48172552016-04-04 Transfer of Viral Communities between Human Individuals during Fecal Microbiota Transplantation Chehoud, Christel Dryga, Anatoly Hwang, Young Nagy-Szakal, Dorottya Hollister, Emily B. Luna, Ruth Ann Versalovic, James Kellermayer, Richard Bushman, Frederic D. mBio Research Article Fecal microbiota transplantation (FMT) is a highly effective treatment for refractory Clostridium difficile infections. However, concerns persist about unwanted cotransfer of pathogenic microbes such as viruses. Here we studed FMT from a single healthy human donor to three pediatric ulcerative colitis patients, each of whom received a course of 22 to 30 FMT treatments. Viral particles were purified from donor and recipient stool samples and sequenced; the reads were then assembled into contigs corresponding to viral genomes or partial genomes. Transfer of selected viruses was confirmed by quantitative PCR. Viral contigs present in the donor could be readily detected in recipients, with up to 32 different donor viral contigs appearing in a recipient sample. Reassuringly, none of these were viruses are known to replicate on human cells. Instead, viral contigs either scored as bacteriophage or could not be attributed taxonomically, suggestive of unstudied phage. The two most frequently transferred gene types were associated with temperate-phage replication. In addition, members of Siphoviridae, the group of typically temperate phages that includes phage lambda, were found to be transferred with significantly greater efficiency than other groups. On the basis of these findings, we propose that the temperate-phage replication style may promote efficient phage transfer between human individuals. In summary, we documented transfer of multiple viral lineages between human individuals through FMT, but in this case series, none were from viral groups known to infect human cells. American Society of Microbiology 2016-03-29 /pmc/articles/PMC4817255/ /pubmed/27025251 http://dx.doi.org/10.1128/mBio.00322-16 Text en Copyright © 2016 Chehoud et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Chehoud, Christel Dryga, Anatoly Hwang, Young Nagy-Szakal, Dorottya Hollister, Emily B. Luna, Ruth Ann Versalovic, James Kellermayer, Richard Bushman, Frederic D. Transfer of Viral Communities between Human Individuals during Fecal Microbiota Transplantation |
title | Transfer of Viral Communities between Human Individuals during Fecal Microbiota Transplantation |
title_full | Transfer of Viral Communities between Human Individuals during Fecal Microbiota Transplantation |
title_fullStr | Transfer of Viral Communities between Human Individuals during Fecal Microbiota Transplantation |
title_full_unstemmed | Transfer of Viral Communities between Human Individuals during Fecal Microbiota Transplantation |
title_short | Transfer of Viral Communities between Human Individuals during Fecal Microbiota Transplantation |
title_sort | transfer of viral communities between human individuals during fecal microbiota transplantation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4817255/ https://www.ncbi.nlm.nih.gov/pubmed/27025251 http://dx.doi.org/10.1128/mBio.00322-16 |
work_keys_str_mv | AT chehoudchristel transferofviralcommunitiesbetweenhumanindividualsduringfecalmicrobiotatransplantation AT drygaanatoly transferofviralcommunitiesbetweenhumanindividualsduringfecalmicrobiotatransplantation AT hwangyoung transferofviralcommunitiesbetweenhumanindividualsduringfecalmicrobiotatransplantation AT nagyszakaldorottya transferofviralcommunitiesbetweenhumanindividualsduringfecalmicrobiotatransplantation AT hollisteremilyb transferofviralcommunitiesbetweenhumanindividualsduringfecalmicrobiotatransplantation AT lunaruthann transferofviralcommunitiesbetweenhumanindividualsduringfecalmicrobiotatransplantation AT versalovicjames transferofviralcommunitiesbetweenhumanindividualsduringfecalmicrobiotatransplantation AT kellermayerrichard transferofviralcommunitiesbetweenhumanindividualsduringfecalmicrobiotatransplantation AT bushmanfredericd transferofviralcommunitiesbetweenhumanindividualsduringfecalmicrobiotatransplantation |