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Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser
An experimental system for serial femtosecond crystallography using an X-ray free-electron laser (XFEL) has been developed. It basically consists of a sample chamber, fluid injectors and a two-dimensional detector. The chamber and the injectors are operated under helium atmosphere at 1 atm. The ambi...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
International Union of Crystallography
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4817517/ https://www.ncbi.nlm.nih.gov/pubmed/25931065 http://dx.doi.org/10.1107/S1600577515004464 |
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| author | Tono, Kensuke Nango, Eriko Sugahara, Michihiro Song, Changyong Park, Jaehyun Tanaka, Tomoyuki Tanaka, Rie Joti, Yasumasa Kameshima, Takashi Ono, Shun Hatsui, Takaki Mizohata, Eiichi Suzuki, Mamoru Shimamura, Tatsuro Tanaka, Yoshiki Iwata, So Yabashi, Makina |
| author_facet | Tono, Kensuke Nango, Eriko Sugahara, Michihiro Song, Changyong Park, Jaehyun Tanaka, Tomoyuki Tanaka, Rie Joti, Yasumasa Kameshima, Takashi Ono, Shun Hatsui, Takaki Mizohata, Eiichi Suzuki, Mamoru Shimamura, Tatsuro Tanaka, Yoshiki Iwata, So Yabashi, Makina |
| author_sort | Tono, Kensuke |
| collection | PubMed |
| description | An experimental system for serial femtosecond crystallography using an X-ray free-electron laser (XFEL) has been developed. It basically consists of a sample chamber, fluid injectors and a two-dimensional detector. The chamber and the injectors are operated under helium atmosphere at 1 atm. The ambient pressure operation facilitates applications to fluid samples. Three kinds of injectors are employed to feed randomly oriented crystals in aqueous solution or highly viscous fluid. Experiments on lysozyme crystals were performed by using the 10 keV XFEL of the SPring-8 Angstrom Compact free-electron LAser (SACLA). The structure of model protein lysozyme from 1 µm crystals at a resolution of 2.4 Å was obtained. |
| format | Online Article Text |
| id | pubmed-4817517 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2015 |
| publisher | International Union of Crystallography |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-48175172016-04-04 Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser Tono, Kensuke Nango, Eriko Sugahara, Michihiro Song, Changyong Park, Jaehyun Tanaka, Tomoyuki Tanaka, Rie Joti, Yasumasa Kameshima, Takashi Ono, Shun Hatsui, Takaki Mizohata, Eiichi Suzuki, Mamoru Shimamura, Tatsuro Tanaka, Yoshiki Iwata, So Yabashi, Makina J Synchrotron Radiat Free-Electron Lasers An experimental system for serial femtosecond crystallography using an X-ray free-electron laser (XFEL) has been developed. It basically consists of a sample chamber, fluid injectors and a two-dimensional detector. The chamber and the injectors are operated under helium atmosphere at 1 atm. The ambient pressure operation facilitates applications to fluid samples. Three kinds of injectors are employed to feed randomly oriented crystals in aqueous solution or highly viscous fluid. Experiments on lysozyme crystals were performed by using the 10 keV XFEL of the SPring-8 Angstrom Compact free-electron LAser (SACLA). The structure of model protein lysozyme from 1 µm crystals at a resolution of 2.4 Å was obtained. International Union of Crystallography 2015-04-16 /pmc/articles/PMC4817517/ /pubmed/25931065 http://dx.doi.org/10.1107/S1600577515004464 Text en © Kensuke Tono et al. 2015 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
| spellingShingle | Free-Electron Lasers Tono, Kensuke Nango, Eriko Sugahara, Michihiro Song, Changyong Park, Jaehyun Tanaka, Tomoyuki Tanaka, Rie Joti, Yasumasa Kameshima, Takashi Ono, Shun Hatsui, Takaki Mizohata, Eiichi Suzuki, Mamoru Shimamura, Tatsuro Tanaka, Yoshiki Iwata, So Yabashi, Makina Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser |
| title | Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser |
| title_full | Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser |
| title_fullStr | Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser |
| title_full_unstemmed | Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser |
| title_short | Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser |
| title_sort | diverse application platform for hard x-ray diffraction in sacla (daphnis): application to serial protein crystallography using an x-ray free-electron laser |
| topic | Free-Electron Lasers |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4817517/ https://www.ncbi.nlm.nih.gov/pubmed/25931065 http://dx.doi.org/10.1107/S1600577515004464 |
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