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Molecular epidemiology and population structure of the honey bee brood pathogen Melissococcus plutonius

Melissococcus plutonius is the causative agent of European foulbrood (EFB), which is a serious brood disease of the European honey bee (Apis mellifera). EFB remains a threat because of a poor understanding of disease epidemiology. We used a recently published multi-locus sequence typing method to ch...

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Autores principales: Budge, Giles E, Shirley, Mark D F, Jones, Benjamin, Quill, Emiline, Tomkies, Victoria, Feil, Edward J, Brown, Mike A, Haynes, Edward G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4817608/
https://www.ncbi.nlm.nih.gov/pubmed/24599072
http://dx.doi.org/10.1038/ismej.2014.20
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author Budge, Giles E
Shirley, Mark D F
Jones, Benjamin
Quill, Emiline
Tomkies, Victoria
Feil, Edward J
Brown, Mike A
Haynes, Edward G
author_facet Budge, Giles E
Shirley, Mark D F
Jones, Benjamin
Quill, Emiline
Tomkies, Victoria
Feil, Edward J
Brown, Mike A
Haynes, Edward G
author_sort Budge, Giles E
collection PubMed
description Melissococcus plutonius is the causative agent of European foulbrood (EFB), which is a serious brood disease of the European honey bee (Apis mellifera). EFB remains a threat because of a poor understanding of disease epidemiology. We used a recently published multi-locus sequence typing method to characterise 206 M. plutonius isolates recovered from outbreaks in England and Wales over the course of 2 years. We detected 15 different sequence types (STs), which were resolved by eBURST and phylogenetic analysis into three clonal complexes (CCs) 3, 12 and 13. Single and double locus variants within CC3 were the most abundant and widespread genotypes, accounting for 85% of the cases. In contrast, CCs 12 and 13 were rarer and predominantly found in geographical regions of high sampling intensity, consistent with a more recent introduction and localised spread. K-function analysis and interpoint distance tests revealed significant geographical clustering in five common STs, but pointed to different dispersal patterns between STs. We noted that CCs appeared to vary in pathogenicity and that infection caused by the more pathogenic variants is more likely to lead to honey bee colony destruction, as opposed to treatment. The importance of these findings for improving our understanding of disease aetiology and control are discussed.
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spelling pubmed-48176082016-04-15 Molecular epidemiology and population structure of the honey bee brood pathogen Melissococcus plutonius Budge, Giles E Shirley, Mark D F Jones, Benjamin Quill, Emiline Tomkies, Victoria Feil, Edward J Brown, Mike A Haynes, Edward G ISME J Original Article Melissococcus plutonius is the causative agent of European foulbrood (EFB), which is a serious brood disease of the European honey bee (Apis mellifera). EFB remains a threat because of a poor understanding of disease epidemiology. We used a recently published multi-locus sequence typing method to characterise 206 M. plutonius isolates recovered from outbreaks in England and Wales over the course of 2 years. We detected 15 different sequence types (STs), which were resolved by eBURST and phylogenetic analysis into three clonal complexes (CCs) 3, 12 and 13. Single and double locus variants within CC3 were the most abundant and widespread genotypes, accounting for 85% of the cases. In contrast, CCs 12 and 13 were rarer and predominantly found in geographical regions of high sampling intensity, consistent with a more recent introduction and localised spread. K-function analysis and interpoint distance tests revealed significant geographical clustering in five common STs, but pointed to different dispersal patterns between STs. We noted that CCs appeared to vary in pathogenicity and that infection caused by the more pathogenic variants is more likely to lead to honey bee colony destruction, as opposed to treatment. The importance of these findings for improving our understanding of disease aetiology and control are discussed. Nature Publishing Group 2014-08 2014-03-06 /pmc/articles/PMC4817608/ /pubmed/24599072 http://dx.doi.org/10.1038/ismej.2014.20 Text en Copyright © 2014 International Society for Microbial Ecology http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Original Article
Budge, Giles E
Shirley, Mark D F
Jones, Benjamin
Quill, Emiline
Tomkies, Victoria
Feil, Edward J
Brown, Mike A
Haynes, Edward G
Molecular epidemiology and population structure of the honey bee brood pathogen Melissococcus plutonius
title Molecular epidemiology and population structure of the honey bee brood pathogen Melissococcus plutonius
title_full Molecular epidemiology and population structure of the honey bee brood pathogen Melissococcus plutonius
title_fullStr Molecular epidemiology and population structure of the honey bee brood pathogen Melissococcus plutonius
title_full_unstemmed Molecular epidemiology and population structure of the honey bee brood pathogen Melissococcus plutonius
title_short Molecular epidemiology and population structure of the honey bee brood pathogen Melissococcus plutonius
title_sort molecular epidemiology and population structure of the honey bee brood pathogen melissococcus plutonius
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4817608/
https://www.ncbi.nlm.nih.gov/pubmed/24599072
http://dx.doi.org/10.1038/ismej.2014.20
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