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Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene
Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. The transgenic cotton (Gossypium hirsutum cv. Coker 310) plants were developed by using βC1 gene in antisense orientation gene driven by Cauliflower mosaic virus...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818328/ https://www.ncbi.nlm.nih.gov/pubmed/27081361 http://dx.doi.org/10.1016/j.sjbs.2014.11.013 |
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author | Sohrab, Sayed Sartaj Kamal, Mohammad A. Ilah, Abdul Husen, Azamal Bhattacharya, P.S. Rana, D. |
author_facet | Sohrab, Sayed Sartaj Kamal, Mohammad A. Ilah, Abdul Husen, Azamal Bhattacharya, P.S. Rana, D. |
author_sort | Sohrab, Sayed Sartaj |
collection | PubMed |
description | Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. The transgenic cotton (Gossypium hirsutum cv. Coker 310) plants were developed by using βC1 gene in antisense orientation gene driven by Cauliflower mosaic virus-35S promoter and nos (nopaline synthase) terminator and mediated by Agrobacterium tumefaciens transformation and somatic embryogenesis system. Molecular confirmation of the transformants was carried out by polymerase chain reaction (PCR) and Southern blot hybridization. The developed transgenic and inoculated plants remained symptomless till their growth period. In conclusion, the plants were observed as resistant to CLCuV. |
format | Online Article Text |
id | pubmed-4818328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-48183282016-04-14 Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene Sohrab, Sayed Sartaj Kamal, Mohammad A. Ilah, Abdul Husen, Azamal Bhattacharya, P.S. Rana, D. Saudi J Biol Sci Original Article Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. The transgenic cotton (Gossypium hirsutum cv. Coker 310) plants were developed by using βC1 gene in antisense orientation gene driven by Cauliflower mosaic virus-35S promoter and nos (nopaline synthase) terminator and mediated by Agrobacterium tumefaciens transformation and somatic embryogenesis system. Molecular confirmation of the transformants was carried out by polymerase chain reaction (PCR) and Southern blot hybridization. The developed transgenic and inoculated plants remained symptomless till their growth period. In conclusion, the plants were observed as resistant to CLCuV. Elsevier 2016-05 2014-11-20 /pmc/articles/PMC4818328/ /pubmed/27081361 http://dx.doi.org/10.1016/j.sjbs.2014.11.013 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Original Article Sohrab, Sayed Sartaj Kamal, Mohammad A. Ilah, Abdul Husen, Azamal Bhattacharya, P.S. Rana, D. Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene |
title | Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene |
title_full | Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene |
title_fullStr | Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene |
title_full_unstemmed | Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene |
title_short | Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene |
title_sort | development of cotton leaf curl virus resistant transgenic cotton using antisense ßc1 gene |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818328/ https://www.ncbi.nlm.nih.gov/pubmed/27081361 http://dx.doi.org/10.1016/j.sjbs.2014.11.013 |
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