Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene

Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. The transgenic cotton (Gossypium hirsutum cv. Coker 310) plants were developed by using βC1 gene in antisense orientation gene driven by Cauliflower mosaic virus...

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Detalles Bibliográficos
Autores principales: Sohrab, Sayed Sartaj, Kamal, Mohammad A., Ilah, Abdul, Husen, Azamal, Bhattacharya, P.S., Rana, D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818328/
https://www.ncbi.nlm.nih.gov/pubmed/27081361
http://dx.doi.org/10.1016/j.sjbs.2014.11.013
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author Sohrab, Sayed Sartaj
Kamal, Mohammad A.
Ilah, Abdul
Husen, Azamal
Bhattacharya, P.S.
Rana, D.
author_facet Sohrab, Sayed Sartaj
Kamal, Mohammad A.
Ilah, Abdul
Husen, Azamal
Bhattacharya, P.S.
Rana, D.
author_sort Sohrab, Sayed Sartaj
collection PubMed
description Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. The transgenic cotton (Gossypium hirsutum cv. Coker 310) plants were developed by using βC1 gene in antisense orientation gene driven by Cauliflower mosaic virus-35S promoter and nos (nopaline synthase) terminator and mediated by Agrobacterium tumefaciens transformation and somatic embryogenesis system. Molecular confirmation of the transformants was carried out by polymerase chain reaction (PCR) and Southern blot hybridization. The developed transgenic and inoculated plants remained symptomless till their growth period. In conclusion, the plants were observed as resistant to CLCuV.
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spelling pubmed-48183282016-04-14 Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene Sohrab, Sayed Sartaj Kamal, Mohammad A. Ilah, Abdul Husen, Azamal Bhattacharya, P.S. Rana, D. Saudi J Biol Sci Original Article Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. The transgenic cotton (Gossypium hirsutum cv. Coker 310) plants were developed by using βC1 gene in antisense orientation gene driven by Cauliflower mosaic virus-35S promoter and nos (nopaline synthase) terminator and mediated by Agrobacterium tumefaciens transformation and somatic embryogenesis system. Molecular confirmation of the transformants was carried out by polymerase chain reaction (PCR) and Southern blot hybridization. The developed transgenic and inoculated plants remained symptomless till their growth period. In conclusion, the plants were observed as resistant to CLCuV. Elsevier 2016-05 2014-11-20 /pmc/articles/PMC4818328/ /pubmed/27081361 http://dx.doi.org/10.1016/j.sjbs.2014.11.013 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Original Article
Sohrab, Sayed Sartaj
Kamal, Mohammad A.
Ilah, Abdul
Husen, Azamal
Bhattacharya, P.S.
Rana, D.
Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene
title Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene
title_full Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene
title_fullStr Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene
title_full_unstemmed Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene
title_short Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene
title_sort development of cotton leaf curl virus resistant transgenic cotton using antisense ßc1 gene
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818328/
https://www.ncbi.nlm.nih.gov/pubmed/27081361
http://dx.doi.org/10.1016/j.sjbs.2014.11.013
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