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Epistatic interactions between at least three loci determine the “rat-tail” phenotype in cattle

BACKGROUND: The “rat-tail” syndrome (RTS) is an inherited hypotrichosis in cattle, which is exclusively expressed in diluted coloured hair. The affected animals also suffer from disturbed thermoregulation, which impairs their health and growth performance. Phenotypic features that are similar to RTS...

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Autores principales: Knaust, Jacqueline, Hadlich, Frieder, Weikard, Rosemarie, Kuehn, Christa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818457/
https://www.ncbi.nlm.nih.gov/pubmed/27037038
http://dx.doi.org/10.1186/s12711-016-0199-8
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author Knaust, Jacqueline
Hadlich, Frieder
Weikard, Rosemarie
Kuehn, Christa
author_facet Knaust, Jacqueline
Hadlich, Frieder
Weikard, Rosemarie
Kuehn, Christa
author_sort Knaust, Jacqueline
collection PubMed
description BACKGROUND: The “rat-tail” syndrome (RTS) is an inherited hypotrichosis in cattle, which is exclusively expressed in diluted coloured hair. The affected animals also suffer from disturbed thermoregulation, which impairs their health and growth performance. Phenotypic features that are similar to RTS are observed in dogs with black hair follicle dysplasia. RESULTS: We used a resource cross population between German Holstein and Charolais cattle breeds to prove that epistatic interactions between at least three independent genetic loci are required for the expression of the RTS phenotype. In this population, the RTS is exclusively expressed in animals with a eumelanic background that is due to the dominant E(D) allele at the melanocortin 1 receptor gene located on Bos taurus autosome (BTA) 18. In addition, only the individuals that are heterozygous at the dilution locus on BTA5 that corresponds to the premelanosome protein or silver gene variant c.64G>A were classified as displaying a RTS phenotype. Linkage and whole-genome association analyses using different models and different pedigrees allowed us to map a third locus (hereafter referred to as the RTS locus) that is essential for the expression of the RTS phenotype to the chromosomal region between 14 and 22 Mb on BTA5. Our findings clearly demonstrate that the RTS and dilution loci are distinct loci on BTA5. CONCLUSIONS: Our study provides evidence that the RTS locus has effects on hair conformation and coat colour dilution and that the effect on coat colour dilution is clearly independent from that of the dilution locus. Finally, our results excluded several other loci that were previously reported to be associated with or to underlie hair conformation or pigmentation traits as the causal mutations of RTS and also several major functional candidate genes that are associated with hypotrichosis in humans. Our finding on the identification of a three-locus interaction that underlies RTS provides a prime example of epistatic interaction between several independent loci that is required for the expression of a distinct phenotype. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12711-016-0199-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-48184572016-04-03 Epistatic interactions between at least three loci determine the “rat-tail” phenotype in cattle Knaust, Jacqueline Hadlich, Frieder Weikard, Rosemarie Kuehn, Christa Genet Sel Evol Research Article BACKGROUND: The “rat-tail” syndrome (RTS) is an inherited hypotrichosis in cattle, which is exclusively expressed in diluted coloured hair. The affected animals also suffer from disturbed thermoregulation, which impairs their health and growth performance. Phenotypic features that are similar to RTS are observed in dogs with black hair follicle dysplasia. RESULTS: We used a resource cross population between German Holstein and Charolais cattle breeds to prove that epistatic interactions between at least three independent genetic loci are required for the expression of the RTS phenotype. In this population, the RTS is exclusively expressed in animals with a eumelanic background that is due to the dominant E(D) allele at the melanocortin 1 receptor gene located on Bos taurus autosome (BTA) 18. In addition, only the individuals that are heterozygous at the dilution locus on BTA5 that corresponds to the premelanosome protein or silver gene variant c.64G>A were classified as displaying a RTS phenotype. Linkage and whole-genome association analyses using different models and different pedigrees allowed us to map a third locus (hereafter referred to as the RTS locus) that is essential for the expression of the RTS phenotype to the chromosomal region between 14 and 22 Mb on BTA5. Our findings clearly demonstrate that the RTS and dilution loci are distinct loci on BTA5. CONCLUSIONS: Our study provides evidence that the RTS locus has effects on hair conformation and coat colour dilution and that the effect on coat colour dilution is clearly independent from that of the dilution locus. Finally, our results excluded several other loci that were previously reported to be associated with or to underlie hair conformation or pigmentation traits as the causal mutations of RTS and also several major functional candidate genes that are associated with hypotrichosis in humans. Our finding on the identification of a three-locus interaction that underlies RTS provides a prime example of epistatic interaction between several independent loci that is required for the expression of a distinct phenotype. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12711-016-0199-8) contains supplementary material, which is available to authorized users. BioMed Central 2016-03-31 /pmc/articles/PMC4818457/ /pubmed/27037038 http://dx.doi.org/10.1186/s12711-016-0199-8 Text en © Knaust et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Knaust, Jacqueline
Hadlich, Frieder
Weikard, Rosemarie
Kuehn, Christa
Epistatic interactions between at least three loci determine the “rat-tail” phenotype in cattle
title Epistatic interactions between at least three loci determine the “rat-tail” phenotype in cattle
title_full Epistatic interactions between at least three loci determine the “rat-tail” phenotype in cattle
title_fullStr Epistatic interactions between at least three loci determine the “rat-tail” phenotype in cattle
title_full_unstemmed Epistatic interactions between at least three loci determine the “rat-tail” phenotype in cattle
title_short Epistatic interactions between at least three loci determine the “rat-tail” phenotype in cattle
title_sort epistatic interactions between at least three loci determine the “rat-tail” phenotype in cattle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818457/
https://www.ncbi.nlm.nih.gov/pubmed/27037038
http://dx.doi.org/10.1186/s12711-016-0199-8
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