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Microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase

BACKGROUND: Propane, a major component of liquid petroleum gas (LPG) derived from fossil fuels, has widespread applications in vehicles, cooking, and ambient heating. Given the concerns about fossil fuel depletion and carbon emission, exploiting alternative and renewable source of propane have becom...

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Autores principales: Zhang, Lei, Liang, Yajing, Wu, Wei, Tan, Xiaoming, Lu, Xuefeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818529/
https://www.ncbi.nlm.nih.gov/pubmed/27042209
http://dx.doi.org/10.1186/s13068-016-0496-z
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author Zhang, Lei
Liang, Yajing
Wu, Wei
Tan, Xiaoming
Lu, Xuefeng
author_facet Zhang, Lei
Liang, Yajing
Wu, Wei
Tan, Xiaoming
Lu, Xuefeng
author_sort Zhang, Lei
collection PubMed
description BACKGROUND: Propane, a major component of liquid petroleum gas (LPG) derived from fossil fuels, has widespread applications in vehicles, cooking, and ambient heating. Given the concerns about fossil fuel depletion and carbon emission, exploiting alternative and renewable source of propane have become attractive. In this study, we report the construction of a novel propane biosynthetic pathway in Escherichia coli. RESULTS: We constructed an aldehyde reductases (ALR)-deprived E. coli strain BW25113(DE3) Δ13 via genetic engineering, which produced sufficient isobutyraldehyde precursors and finally achieved de novo synthesis of propane (91 μg/L) by assembling the engineered valine pathway and cyanobacterial aldehyde-deformylating oxygenase (ADO). Additionally, after extensive screening of ADO mutants generated by engineering the active center to accommodate branched-chain isobutyraldehyde, we identified two ADO mutants (I127G, I127G/A48G) which exhibited higher catalytic activity for isobutyraldehyde and improved propane productivity by three times (267 μg/L). CONCLUSIONS: The propane biosynthetic pathway constructed here through the engineered valine pathway can produce abundant isobutyraldehyde for ADO and overcome the low availability of precursors in propane production. Furthermore, the rational design aiming at the ADO active center illustrates the plasticity and catalytic potential of ADO. These results together highlight the potential for developing a microbial biomanufacturing platform for propane. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13068-016-0496-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-48185292016-04-03 Microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase Zhang, Lei Liang, Yajing Wu, Wei Tan, Xiaoming Lu, Xuefeng Biotechnol Biofuels Research BACKGROUND: Propane, a major component of liquid petroleum gas (LPG) derived from fossil fuels, has widespread applications in vehicles, cooking, and ambient heating. Given the concerns about fossil fuel depletion and carbon emission, exploiting alternative and renewable source of propane have become attractive. In this study, we report the construction of a novel propane biosynthetic pathway in Escherichia coli. RESULTS: We constructed an aldehyde reductases (ALR)-deprived E. coli strain BW25113(DE3) Δ13 via genetic engineering, which produced sufficient isobutyraldehyde precursors and finally achieved de novo synthesis of propane (91 μg/L) by assembling the engineered valine pathway and cyanobacterial aldehyde-deformylating oxygenase (ADO). Additionally, after extensive screening of ADO mutants generated by engineering the active center to accommodate branched-chain isobutyraldehyde, we identified two ADO mutants (I127G, I127G/A48G) which exhibited higher catalytic activity for isobutyraldehyde and improved propane productivity by three times (267 μg/L). CONCLUSIONS: The propane biosynthetic pathway constructed here through the engineered valine pathway can produce abundant isobutyraldehyde for ADO and overcome the low availability of precursors in propane production. Furthermore, the rational design aiming at the ADO active center illustrates the plasticity and catalytic potential of ADO. These results together highlight the potential for developing a microbial biomanufacturing platform for propane. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13068-016-0496-z) contains supplementary material, which is available to authorized users. BioMed Central 2016-04-01 /pmc/articles/PMC4818529/ /pubmed/27042209 http://dx.doi.org/10.1186/s13068-016-0496-z Text en © Zhang et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhang, Lei
Liang, Yajing
Wu, Wei
Tan, Xiaoming
Lu, Xuefeng
Microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase
title Microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase
title_full Microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase
title_fullStr Microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase
title_full_unstemmed Microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase
title_short Microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase
title_sort microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818529/
https://www.ncbi.nlm.nih.gov/pubmed/27042209
http://dx.doi.org/10.1186/s13068-016-0496-z
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