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The key role of CYC2 during meiosis in Tetrahymena thermophila

Meiotic recombination is carried out through a specialized pathway for the formation and repair of DNA double-strand breaks (DSBs) made by the Spo11 protein. The present study shed light on the functional role of cyclin, CYC2, in Tetrahymena thermophila which has transcriptionally high expression le...

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Autores principales: Xu, Qianlan, Wang, Ruoyu, Ghanam, A. R., Yan, Guanxiong, Miao, Wei, Song, Xiaoyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Higher Education Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818844/
https://www.ncbi.nlm.nih.gov/pubmed/27008457
http://dx.doi.org/10.1007/s13238-016-0254-9
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author Xu, Qianlan
Wang, Ruoyu
Ghanam, A. R.
Yan, Guanxiong
Miao, Wei
Song, Xiaoyuan
author_facet Xu, Qianlan
Wang, Ruoyu
Ghanam, A. R.
Yan, Guanxiong
Miao, Wei
Song, Xiaoyuan
author_sort Xu, Qianlan
collection PubMed
description Meiotic recombination is carried out through a specialized pathway for the formation and repair of DNA double-strand breaks (DSBs) made by the Spo11 protein. The present study shed light on the functional role of cyclin, CYC2, in Tetrahymena thermophila which has transcriptionally high expression level during meiosis process. Knocking out the CYC2 gene results in arrest of meiotic conjugation process at 2.5–3.5 h after conjugation initiation, before the meiosis division starts, and in company with the absence of DSBs. To investigate the underlying mechanism of this phenomenon, a complete transcriptome profile was performed between wild-type strain and CYC2 knock-out strain. Functional analysis of RNA-Seq results identifies related differentially expressed genes (DEGs) including SPO11 and these DEGs are enriched in DNA repair/mismatch repair (MMR) terms in homologous recombination (HR), which indicates that CYC2 could play a crucial role in meiosis by regulating SPO11 and participating in HR. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13238-016-0254-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-48188442016-04-04 The key role of CYC2 during meiosis in Tetrahymena thermophila Xu, Qianlan Wang, Ruoyu Ghanam, A. R. Yan, Guanxiong Miao, Wei Song, Xiaoyuan Protein Cell Research Article Meiotic recombination is carried out through a specialized pathway for the formation and repair of DNA double-strand breaks (DSBs) made by the Spo11 protein. The present study shed light on the functional role of cyclin, CYC2, in Tetrahymena thermophila which has transcriptionally high expression level during meiosis process. Knocking out the CYC2 gene results in arrest of meiotic conjugation process at 2.5–3.5 h after conjugation initiation, before the meiosis division starts, and in company with the absence of DSBs. To investigate the underlying mechanism of this phenomenon, a complete transcriptome profile was performed between wild-type strain and CYC2 knock-out strain. Functional analysis of RNA-Seq results identifies related differentially expressed genes (DEGs) including SPO11 and these DEGs are enriched in DNA repair/mismatch repair (MMR) terms in homologous recombination (HR), which indicates that CYC2 could play a crucial role in meiosis by regulating SPO11 and participating in HR. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13238-016-0254-9) contains supplementary material, which is available to authorized users. Higher Education Press 2016-03-23 2016-04 /pmc/articles/PMC4818844/ /pubmed/27008457 http://dx.doi.org/10.1007/s13238-016-0254-9 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research Article
Xu, Qianlan
Wang, Ruoyu
Ghanam, A. R.
Yan, Guanxiong
Miao, Wei
Song, Xiaoyuan
The key role of CYC2 during meiosis in Tetrahymena thermophila
title The key role of CYC2 during meiosis in Tetrahymena thermophila
title_full The key role of CYC2 during meiosis in Tetrahymena thermophila
title_fullStr The key role of CYC2 during meiosis in Tetrahymena thermophila
title_full_unstemmed The key role of CYC2 during meiosis in Tetrahymena thermophila
title_short The key role of CYC2 during meiosis in Tetrahymena thermophila
title_sort key role of cyc2 during meiosis in tetrahymena thermophila
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818844/
https://www.ncbi.nlm.nih.gov/pubmed/27008457
http://dx.doi.org/10.1007/s13238-016-0254-9
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