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Avian leukosis virus subgroup J induces its receptor--chNHE1 up-regulation
BACKGROUND: Avian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus which causes immunosuppression and neoplasia in meat-type and egg-type chickens. ALV-J infects host cells via specific interaction between the viral Env and the cell surface receptor —chicken sodium hydrogen exchanger typ...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818912/ https://www.ncbi.nlm.nih.gov/pubmed/27039379 http://dx.doi.org/10.1186/s12985-016-0517-3 |
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author | Feng, Weiguo Meng, Wei Cai, Liming Cui, Xiyao Pan, Zhifang Wang, Guihua Cheng, Ziqiang |
author_facet | Feng, Weiguo Meng, Wei Cai, Liming Cui, Xiyao Pan, Zhifang Wang, Guihua Cheng, Ziqiang |
author_sort | Feng, Weiguo |
collection | PubMed |
description | BACKGROUND: Avian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus which causes immunosuppression and neoplasia in meat-type and egg-type chickens. ALV-J infects host cells via specific interaction between the viral Env and the cell surface receptor —chicken sodium hydrogen exchanger type 1 (chNHE1). NHE1 involved in altering the cellular pH and playing a critical role in tumorigenesis. However, little is known about the other relationship between ALV-J and chNHE1. METHODS AND RESULTS: In ALV-J infected DF-1 cells, the mRNA level of chNHE1 was up-regulated with time-dependent manner tested by real time PCR, and accordingly, intracellular pH was increased tested by spectrofluorometer. In vivo, the mRNA level of chNHE1 was determined by real time PCR in ALV-J infected experimental chickens and field cases. The result showed that the mRNA level of chNHE1 was up-regulated after virus shedding, especially in continuous viremic shedders (CS group). However, no significant difference was found between non-shedding group (NS group) and control group. In field cases, mRNA level of chNHE1 was positively correlated with increasing ALV-J load in tumor bearing and immune tolerance chickens. Furthermore, immunohistochemistry results showed that the protein expression of chNHE1 was up-regulated in different organs of both experimental chickens and tumor bearing chickens compared with the control. CONCLUSION: Taken together, we conclude that ALV-J induces chNHE1 up-regulation in viremia and neoplasia chickens. |
format | Online Article Text |
id | pubmed-4818912 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-48189122016-04-04 Avian leukosis virus subgroup J induces its receptor--chNHE1 up-regulation Feng, Weiguo Meng, Wei Cai, Liming Cui, Xiyao Pan, Zhifang Wang, Guihua Cheng, Ziqiang Virol J Research BACKGROUND: Avian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus which causes immunosuppression and neoplasia in meat-type and egg-type chickens. ALV-J infects host cells via specific interaction between the viral Env and the cell surface receptor —chicken sodium hydrogen exchanger type 1 (chNHE1). NHE1 involved in altering the cellular pH and playing a critical role in tumorigenesis. However, little is known about the other relationship between ALV-J and chNHE1. METHODS AND RESULTS: In ALV-J infected DF-1 cells, the mRNA level of chNHE1 was up-regulated with time-dependent manner tested by real time PCR, and accordingly, intracellular pH was increased tested by spectrofluorometer. In vivo, the mRNA level of chNHE1 was determined by real time PCR in ALV-J infected experimental chickens and field cases. The result showed that the mRNA level of chNHE1 was up-regulated after virus shedding, especially in continuous viremic shedders (CS group). However, no significant difference was found between non-shedding group (NS group) and control group. In field cases, mRNA level of chNHE1 was positively correlated with increasing ALV-J load in tumor bearing and immune tolerance chickens. Furthermore, immunohistochemistry results showed that the protein expression of chNHE1 was up-regulated in different organs of both experimental chickens and tumor bearing chickens compared with the control. CONCLUSION: Taken together, we conclude that ALV-J induces chNHE1 up-regulation in viremia and neoplasia chickens. BioMed Central 2016-04-02 /pmc/articles/PMC4818912/ /pubmed/27039379 http://dx.doi.org/10.1186/s12985-016-0517-3 Text en © Feng et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Feng, Weiguo Meng, Wei Cai, Liming Cui, Xiyao Pan, Zhifang Wang, Guihua Cheng, Ziqiang Avian leukosis virus subgroup J induces its receptor--chNHE1 up-regulation |
title | Avian leukosis virus subgroup J induces its receptor--chNHE1 up-regulation |
title_full | Avian leukosis virus subgroup J induces its receptor--chNHE1 up-regulation |
title_fullStr | Avian leukosis virus subgroup J induces its receptor--chNHE1 up-regulation |
title_full_unstemmed | Avian leukosis virus subgroup J induces its receptor--chNHE1 up-regulation |
title_short | Avian leukosis virus subgroup J induces its receptor--chNHE1 up-regulation |
title_sort | avian leukosis virus subgroup j induces its receptor--chnhe1 up-regulation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818912/ https://www.ncbi.nlm.nih.gov/pubmed/27039379 http://dx.doi.org/10.1186/s12985-016-0517-3 |
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