Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection

BACKGROUND: Targeted genomic editing using the CRISPR/Cas9 methodology has opened exciting new avenues in probing gene function in virtually any model system, including cultured mammalian cells. Depending on the desired mutation, several experimental options exist in the isolation of clonal lines, s...

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Detalles Bibliográficos
Autores principales: Estep, Jason A., Sternburg, Erin L., Sanchez, Gissell A., Karginov, Fedor V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818936/
https://www.ncbi.nlm.nih.gov/pubmed/27038923
http://dx.doi.org/10.1186/s12867-016-0061-0

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