Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave

For two libraries (PDF1 and ODF1) using Illumina sequencing 44,082,301 and 43,708,132 clean reads were obtained, respectively. After being mapped to the bovine RefSeq database, 15,533 genes were identified to be expressed in both types of follicles (cut-off RPKM > 0.5), of which 719 were highly e...

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Autores principales: Li, Pengfei, Meng, Jinzhu, Liu, Wenzhong, Smith, George W., Yao, Jianbo, Lyu, Lihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819119/
https://www.ncbi.nlm.nih.gov/pubmed/27088081
http://dx.doi.org/10.1155/2016/3472748
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author Li, Pengfei
Meng, Jinzhu
Liu, Wenzhong
Smith, George W.
Yao, Jianbo
Lyu, Lihua
author_facet Li, Pengfei
Meng, Jinzhu
Liu, Wenzhong
Smith, George W.
Yao, Jianbo
Lyu, Lihua
author_sort Li, Pengfei
collection PubMed
description For two libraries (PDF1 and ODF1) using Illumina sequencing 44,082,301 and 43,708,132 clean reads were obtained, respectively. After being mapped to the bovine RefSeq database, 15,533 genes were identified to be expressed in both types of follicles (cut-off RPKM > 0.5), of which 719 were highly expressed in bovine follicles (cut-off RPKM > 100). Furthermore, 83 genes were identified as being differentially expressed in ODF1 versus PDF1, where 42 genes were upregulated and 41 genes were downregulated. KEGG pathway analysis revealed two upregulated genes in ODF1 versus PDF1, CYP11A1, and CYP19A1, which are important genes in the steroid hormone biosynthesis pathway. This study represents the first investigation of transcriptome of bovine follicles at predeviation and onset of deviation stages and provides a foundation for future investigation of the regulatory mechanisms involved in follicular development in cattle.
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spelling pubmed-48191192016-04-17 Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave Li, Pengfei Meng, Jinzhu Liu, Wenzhong Smith, George W. Yao, Jianbo Lyu, Lihua Int J Genomics Research Article For two libraries (PDF1 and ODF1) using Illumina sequencing 44,082,301 and 43,708,132 clean reads were obtained, respectively. After being mapped to the bovine RefSeq database, 15,533 genes were identified to be expressed in both types of follicles (cut-off RPKM > 0.5), of which 719 were highly expressed in bovine follicles (cut-off RPKM > 100). Furthermore, 83 genes were identified as being differentially expressed in ODF1 versus PDF1, where 42 genes were upregulated and 41 genes were downregulated. KEGG pathway analysis revealed two upregulated genes in ODF1 versus PDF1, CYP11A1, and CYP19A1, which are important genes in the steroid hormone biosynthesis pathway. This study represents the first investigation of transcriptome of bovine follicles at predeviation and onset of deviation stages and provides a foundation for future investigation of the regulatory mechanisms involved in follicular development in cattle. Hindawi Publishing Corporation 2016 2016-03-21 /pmc/articles/PMC4819119/ /pubmed/27088081 http://dx.doi.org/10.1155/2016/3472748 Text en Copyright © 2016 Pengfei Li et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Pengfei
Meng, Jinzhu
Liu, Wenzhong
Smith, George W.
Yao, Jianbo
Lyu, Lihua
Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave
title Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave
title_full Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave
title_fullStr Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave
title_full_unstemmed Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave
title_short Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave
title_sort transcriptome analysis of bovine ovarian follicles at predeviation and onset of deviation stages of a follicular wave
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819119/
https://www.ncbi.nlm.nih.gov/pubmed/27088081
http://dx.doi.org/10.1155/2016/3472748
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