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Hyperoside Induces Endogenous Antioxidant System to Alleviate Oxidative Stress

BACKGROUND: Hyperoside, a flavonoid which is mainly found in Hypericum perforatum L., has many biological effects. One of the most important effects is to prevent the oxidative stress induced by reactive oxygen species. However, the molecular mechanisms underlying its effect are not fully understood...

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Autores principales: Park, Ji Young, Han, Xia, Piao, Mei Jing, Oh, Min Chang, Fernando, Pattage Madushan Dilhara Jayatissa, Kang, Kyoung Ah, Ryu, Yea Seong, Jung, Uhee, Kim, In Gyu, Hyun, Jin Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Cancer Prevention 2016
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819665/
https://www.ncbi.nlm.nih.gov/pubmed/27051648
http://dx.doi.org/10.15430/JCP.2016.21.1.41
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author Park, Ji Young
Han, Xia
Piao, Mei Jing
Oh, Min Chang
Fernando, Pattage Madushan Dilhara Jayatissa
Kang, Kyoung Ah
Ryu, Yea Seong
Jung, Uhee
Kim, In Gyu
Hyun, Jin Won
author_facet Park, Ji Young
Han, Xia
Piao, Mei Jing
Oh, Min Chang
Fernando, Pattage Madushan Dilhara Jayatissa
Kang, Kyoung Ah
Ryu, Yea Seong
Jung, Uhee
Kim, In Gyu
Hyun, Jin Won
author_sort Park, Ji Young
collection PubMed
description BACKGROUND: Hyperoside, a flavonoid which is mainly found in Hypericum perforatum L., has many biological effects. One of the most important effects is to prevent the oxidative stress induced by reactive oxygen species. However, the molecular mechanisms underlying its effect are not fully understood. Oxidative stress is implicated in the occurrence of various physical diseases. A wide array of enzymatic antioxidant defense systems include NADH: quinone oxidoreductase 1, superoxide dismutase, and heme oxygenase-1 (HO-1). In the present study, the protective effects of hyperoside against hydrogen peroxide-induced oxidative stress in human lens epithelial cells, HLE-B3, were investigated in terms of HO-1 induction. METHODS: The protein and mRNA expressions of HO-1 were examined by Western blotting and reverse transcriptase-PCR assays, respectively. To evaluate the ability of hyperoside to activate nuclear factor erythroid 2-related factor 2 (Nrf2), Western blotting and electrophoretic mobility shift assay were performed with nuclear extracts prepared from HLE-B3 cells treated with hyperoside. The activation of extracellular signal-regulated kinase (ERK), the upstream kinase of Nrf2 signaling, was monitored by Western blot analysis. The protective effect of hyperoside in HLE-B3 cells against hydrogen peroxide was performed by MTT assay. RESULTS: Hyperoside increased both the mRNA and protein expression of HO-1 in a time- and dose-dependent manner. In addition, hyperoside elevated the level of of Nrf2 and its antioxidant response element-binding activity, which was modulated by upstream of ERK. Moreover, it activated ERK and restored cell viability which was decreased by hydrogen peroxide. CONCLUSIONS: Hyperoside is an effective compound to protect cells against oxidative stress via HO-1 induction.
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spelling pubmed-48196652016-04-05 Hyperoside Induces Endogenous Antioxidant System to Alleviate Oxidative Stress Park, Ji Young Han, Xia Piao, Mei Jing Oh, Min Chang Fernando, Pattage Madushan Dilhara Jayatissa Kang, Kyoung Ah Ryu, Yea Seong Jung, Uhee Kim, In Gyu Hyun, Jin Won J Cancer Prev Original Article BACKGROUND: Hyperoside, a flavonoid which is mainly found in Hypericum perforatum L., has many biological effects. One of the most important effects is to prevent the oxidative stress induced by reactive oxygen species. However, the molecular mechanisms underlying its effect are not fully understood. Oxidative stress is implicated in the occurrence of various physical diseases. A wide array of enzymatic antioxidant defense systems include NADH: quinone oxidoreductase 1, superoxide dismutase, and heme oxygenase-1 (HO-1). In the present study, the protective effects of hyperoside against hydrogen peroxide-induced oxidative stress in human lens epithelial cells, HLE-B3, were investigated in terms of HO-1 induction. METHODS: The protein and mRNA expressions of HO-1 were examined by Western blotting and reverse transcriptase-PCR assays, respectively. To evaluate the ability of hyperoside to activate nuclear factor erythroid 2-related factor 2 (Nrf2), Western blotting and electrophoretic mobility shift assay were performed with nuclear extracts prepared from HLE-B3 cells treated with hyperoside. The activation of extracellular signal-regulated kinase (ERK), the upstream kinase of Nrf2 signaling, was monitored by Western blot analysis. The protective effect of hyperoside in HLE-B3 cells against hydrogen peroxide was performed by MTT assay. RESULTS: Hyperoside increased both the mRNA and protein expression of HO-1 in a time- and dose-dependent manner. In addition, hyperoside elevated the level of of Nrf2 and its antioxidant response element-binding activity, which was modulated by upstream of ERK. Moreover, it activated ERK and restored cell viability which was decreased by hydrogen peroxide. CONCLUSIONS: Hyperoside is an effective compound to protect cells against oxidative stress via HO-1 induction. Korean Society of Cancer Prevention 2016-03 2016-03-30 /pmc/articles/PMC4819665/ /pubmed/27051648 http://dx.doi.org/10.15430/JCP.2016.21.1.41 Text en Copyright © 2016 Korean Society of Cancer Prevention This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Park, Ji Young
Han, Xia
Piao, Mei Jing
Oh, Min Chang
Fernando, Pattage Madushan Dilhara Jayatissa
Kang, Kyoung Ah
Ryu, Yea Seong
Jung, Uhee
Kim, In Gyu
Hyun, Jin Won
Hyperoside Induces Endogenous Antioxidant System to Alleviate Oxidative Stress
title Hyperoside Induces Endogenous Antioxidant System to Alleviate Oxidative Stress
title_full Hyperoside Induces Endogenous Antioxidant System to Alleviate Oxidative Stress
title_fullStr Hyperoside Induces Endogenous Antioxidant System to Alleviate Oxidative Stress
title_full_unstemmed Hyperoside Induces Endogenous Antioxidant System to Alleviate Oxidative Stress
title_short Hyperoside Induces Endogenous Antioxidant System to Alleviate Oxidative Stress
title_sort hyperoside induces endogenous antioxidant system to alleviate oxidative stress
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819665/
https://www.ncbi.nlm.nih.gov/pubmed/27051648
http://dx.doi.org/10.15430/JCP.2016.21.1.41
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