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Gonadotropin ratio affects the in vitro growth of rhesus ovarian preantral follicles

In vitro follicle growth (IVFG) strategy is critical in the fertility preservation of cancer survivors; however, its optimal protocol needs to be developed using primate models since the availability of human samples is limited. Only a few previous studies have reported the successful IVFG of rhesus...

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Autores principales: Kim, Yoon Young, Yun, Jun-Won, Kim, Jong Min, Park, Chung Gyu, Rosenwaks, Zev, Liu, Hung Ching, Kang, Byeong-Cheol, Ku, Seung-Yup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819672/
https://www.ncbi.nlm.nih.gov/pubmed/26980777
http://dx.doi.org/10.1136/jim-2015-000001
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author Kim, Yoon Young
Yun, Jun-Won
Kim, Jong Min
Park, Chung Gyu
Rosenwaks, Zev
Liu, Hung Ching
Kang, Byeong-Cheol
Ku, Seung-Yup
author_facet Kim, Yoon Young
Yun, Jun-Won
Kim, Jong Min
Park, Chung Gyu
Rosenwaks, Zev
Liu, Hung Ching
Kang, Byeong-Cheol
Ku, Seung-Yup
author_sort Kim, Yoon Young
collection PubMed
description In vitro follicle growth (IVFG) strategy is critical in the fertility preservation of cancer survivors; however, its optimal protocol needs to be developed using primate models since the availability of human samples is limited. Only a few previous studies have reported the successful IVFG of rhesus monkey ovaries using low-dose follicle-stimulating hormone (FSH) (0.3 or 3 ng/mL) and long-term culture (up to 5 weeks) and it is still uncertain in regard to the optimal culture duration and effective dose of treated gonadotropins applicable to the IVFG of rhesus preantral follicles. Recently, we have reported that the FSH to luteinizing hormone (LH) ratio affects the in vitro growth of murine ovarian follicles. We aimed to investigate whether gonadotropin ratios affect the efficiency of rhesus follicular growth in vitro. Ovaries were collected from six necropsied rhesus macaques (4–9 years) and preantral follicles were retrieved and cultured for 14 days using 200 mIU/mL FSH. The characteristics of follicular growth were compared between the FSH:LH=1:1 (n=24) and FSH:LH=2:1 (n=24) groups. High concentration gonadotropin treatment shortened the duration required for in vitro maturation of rhesus preantral follicles. The FSH:LH=2:1 group showed a faster follicular growth and enabled the acquisition of mature oocytes, although the expression of growth differentiation factor (GDF)-9 and anti-Müllerian hormone (AMH) did not differ significantly between the two groups. Taken together, high dose gonadotropin treatment can shorten the duration of IVFG and the gonadotropin ratio is important in the IVFG of rhesus monkey ovaries.
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spelling pubmed-48196722016-04-19 Gonadotropin ratio affects the in vitro growth of rhesus ovarian preantral follicles Kim, Yoon Young Yun, Jun-Won Kim, Jong Min Park, Chung Gyu Rosenwaks, Zev Liu, Hung Ching Kang, Byeong-Cheol Ku, Seung-Yup J Investig Med Original Research In vitro follicle growth (IVFG) strategy is critical in the fertility preservation of cancer survivors; however, its optimal protocol needs to be developed using primate models since the availability of human samples is limited. Only a few previous studies have reported the successful IVFG of rhesus monkey ovaries using low-dose follicle-stimulating hormone (FSH) (0.3 or 3 ng/mL) and long-term culture (up to 5 weeks) and it is still uncertain in regard to the optimal culture duration and effective dose of treated gonadotropins applicable to the IVFG of rhesus preantral follicles. Recently, we have reported that the FSH to luteinizing hormone (LH) ratio affects the in vitro growth of murine ovarian follicles. We aimed to investigate whether gonadotropin ratios affect the efficiency of rhesus follicular growth in vitro. Ovaries were collected from six necropsied rhesus macaques (4–9 years) and preantral follicles were retrieved and cultured for 14 days using 200 mIU/mL FSH. The characteristics of follicular growth were compared between the FSH:LH=1:1 (n=24) and FSH:LH=2:1 (n=24) groups. High concentration gonadotropin treatment shortened the duration required for in vitro maturation of rhesus preantral follicles. The FSH:LH=2:1 group showed a faster follicular growth and enabled the acquisition of mature oocytes, although the expression of growth differentiation factor (GDF)-9 and anti-Müllerian hormone (AMH) did not differ significantly between the two groups. Taken together, high dose gonadotropin treatment can shorten the duration of IVFG and the gonadotropin ratio is important in the IVFG of rhesus monkey ovaries. BMJ Publishing Group 2016-04 2016-03-15 /pmc/articles/PMC4819672/ /pubmed/26980777 http://dx.doi.org/10.1136/jim-2015-000001 Text en Copyright © 2016 American Federation for Medical Research This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Original Research
Kim, Yoon Young
Yun, Jun-Won
Kim, Jong Min
Park, Chung Gyu
Rosenwaks, Zev
Liu, Hung Ching
Kang, Byeong-Cheol
Ku, Seung-Yup
Gonadotropin ratio affects the in vitro growth of rhesus ovarian preantral follicles
title Gonadotropin ratio affects the in vitro growth of rhesus ovarian preantral follicles
title_full Gonadotropin ratio affects the in vitro growth of rhesus ovarian preantral follicles
title_fullStr Gonadotropin ratio affects the in vitro growth of rhesus ovarian preantral follicles
title_full_unstemmed Gonadotropin ratio affects the in vitro growth of rhesus ovarian preantral follicles
title_short Gonadotropin ratio affects the in vitro growth of rhesus ovarian preantral follicles
title_sort gonadotropin ratio affects the in vitro growth of rhesus ovarian preantral follicles
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819672/
https://www.ncbi.nlm.nih.gov/pubmed/26980777
http://dx.doi.org/10.1136/jim-2015-000001
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