Genotyping of feline leukemia virus in Mexican housecats

Feline leukemia virus (FeLV) is a retrovirus with variable rates of infection globally. DNA was obtained from cats’ peripheral blood mononuclear cells, and proviral DNA of pol and env genes was detected using PCR. Seventy-six percent of cats scored positive for FeLV using env-PCR; and 54 %, by pol-P...

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Detalles Bibliográficos
Autores principales: Ramírez, Hugo, Autran, Marcela, García, M. Martha, Carmona, M. Ángel, Rodríguez, Cecilia, Martínez, H. Alejandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2016
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819734/
https://www.ncbi.nlm.nih.gov/pubmed/26747244
http://dx.doi.org/10.1007/s00705-015-2740-4
Descripción
Sumario:Feline leukemia virus (FeLV) is a retrovirus with variable rates of infection globally. DNA was obtained from cats’ peripheral blood mononuclear cells, and proviral DNA of pol and env genes was detected using PCR. Seventy-six percent of cats scored positive for FeLV using env-PCR; and 54 %, by pol-PCR. Phylogenetic analysis of both regions identified sequences that correspond to a group that includes endogenous retroviruses. They form an independent branch and, therefore, a new group of endogenous viruses. Cat gender, age, outdoor access, and cohabitation with other cats were found to be significant risk factors associated with the disease. This strongly suggests that these FeLV genotypes are widely distributed in the studied feline population in Mexico.

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