Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis

Objective: Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium. Methods: Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the con...

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Autores principales: Kjelgaard-Petersen, Cecilie, Siebuhr, Anne Sofie, Christiansen, Thorbjørn, Ladel, Christoph, Karsdal, Morten, Bay-Jensen, Anne-Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819844/
https://www.ncbi.nlm.nih.gov/pubmed/26863055
http://dx.doi.org/10.3109/1354750X.2015.1105497
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author Kjelgaard-Petersen, Cecilie
Siebuhr, Anne Sofie
Christiansen, Thorbjørn
Ladel, Christoph
Karsdal, Morten
Bay-Jensen, Anne-Christine
author_facet Kjelgaard-Petersen, Cecilie
Siebuhr, Anne Sofie
Christiansen, Thorbjørn
Ladel, Christoph
Karsdal, Morten
Bay-Jensen, Anne-Christine
author_sort Kjelgaard-Petersen, Cecilie
collection PubMed
description Objective: Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium. Methods: Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the conditioned medium. Results: TNFα significantly increased C1M up to seven-fold (p = 0.0002), C3M up to 24-fold (p = 0.0011), and acMMP3 up to 14-fold (p < 0.0001) in SMEs. IL-1β also significantly increased C1M up to five-fold (p = 0.00094), C3M four-fold (p = 0.007), and acMMP3 18-fold (p < 0.0001) in SMEs. Conclusion: The biomarkers C1M, C3M, and acMMP-3 were synovitis biomarkers ex vivo and provide a translational tool together with the SME model.
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spelling pubmed-48198442016-04-22 Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis Kjelgaard-Petersen, Cecilie Siebuhr, Anne Sofie Christiansen, Thorbjørn Ladel, Christoph Karsdal, Morten Bay-Jensen, Anne-Christine Biomarkers Research Article Objective: Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium. Methods: Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the conditioned medium. Results: TNFα significantly increased C1M up to seven-fold (p = 0.0002), C3M up to 24-fold (p = 0.0011), and acMMP3 up to 14-fold (p < 0.0001) in SMEs. IL-1β also significantly increased C1M up to five-fold (p = 0.00094), C3M four-fold (p = 0.007), and acMMP3 18-fold (p < 0.0001) in SMEs. Conclusion: The biomarkers C1M, C3M, and acMMP-3 were synovitis biomarkers ex vivo and provide a translational tool together with the SME model. Taylor & Francis 2015-11-17 2016-02-10 /pmc/articles/PMC4819844/ /pubmed/26863055 http://dx.doi.org/10.3109/1354750X.2015.1105497 Text en © 2016 The Author(s). Published by Taylor & Francis. http://creativecommons.org/Licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/Licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kjelgaard-Petersen, Cecilie
Siebuhr, Anne Sofie
Christiansen, Thorbjørn
Ladel, Christoph
Karsdal, Morten
Bay-Jensen, Anne-Christine
Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis
title Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis
title_full Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis
title_fullStr Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis
title_full_unstemmed Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis
title_short Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis
title_sort synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819844/
https://www.ncbi.nlm.nih.gov/pubmed/26863055
http://dx.doi.org/10.3109/1354750X.2015.1105497
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