Cargando…

Analysis of Differential Proteins in Two Wing-Type Females of Sogatella furcifera (Hemiptera: Delphacidae)

Sogatella furcifera (Horvath) is an important rice pest with the wing dimorphism, including macropterous and brachypterous morphs. The protein expression profiles in two wing-type adults and two wing-type disc fifth-instar nymphs were analyzed using two-dimensional gel protein electrophoresis and ma...

Descripción completa

Detalles Bibliográficos
Autores principales: Liang, Zi-Qiang, Song, Shao-Yun, Liang, Shi-Ke, Wang, Fang-Hai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819851/
https://www.ncbi.nlm.nih.gov/pubmed/27044649
http://dx.doi.org/10.1093/jisesa/iew024
_version_ 1782425296641720320
author Liang, Zi-Qiang
Song, Shao-Yun
Liang, Shi-Ke
Wang, Fang-Hai
author_facet Liang, Zi-Qiang
Song, Shao-Yun
Liang, Shi-Ke
Wang, Fang-Hai
author_sort Liang, Zi-Qiang
collection PubMed
description Sogatella furcifera (Horvath) is an important rice pest with the wing dimorphism, including macropterous and brachypterous morphs. The protein expression profiles in two wing-type adults and two wing-type disc fifth-instar nymphs were analyzed using two-dimensional gel protein electrophoresis and mass spectrometry. In adults and fifth-instar nymphs, 127 and 162 protein spots were detected, respectively. Fifty-five differentially expressed protein spots were identified between the long-winged adults and the short-winged adults, and 62 differentially expressed protein spots were found between the long-winged disc fifth-instar nymphs and short-winged disc fifth-instar nymphs. In long-winged and short-winged adults, six and seven specific protein spots were identified, respectively, with five and seven protein spots having more than threefold increased level, respectively. In long-winged and short-winged disc morph nymphs, 8 and 12 specific protein spots were identified, respectively, with 11 and 17 spots containing more than threefold increased level, respectively. Among the 16 identified proteins, five proteins are associated with muscle function, suggesting that muscle is a main tissue where the genes were differentially expressed between the two wing types. In addition, the content of a peptidase with an insulinase domain was higher (by 3.02 ± 0.59 fold) in the short-winged fifth-instar nymphs than in the long-winged fifth-instar nymphs, which suggests that this peptidase may be involved in wing differentiation by regulating insulin receptors. The results of this study provide some genetic clues for the wing differential development in S. furcifera and provide more references for future studies.
format Online
Article
Text
id pubmed-4819851
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-48198512016-04-05 Analysis of Differential Proteins in Two Wing-Type Females of Sogatella furcifera (Hemiptera: Delphacidae) Liang, Zi-Qiang Song, Shao-Yun Liang, Shi-Ke Wang, Fang-Hai J Insect Sci Research Article Sogatella furcifera (Horvath) is an important rice pest with the wing dimorphism, including macropterous and brachypterous morphs. The protein expression profiles in two wing-type adults and two wing-type disc fifth-instar nymphs were analyzed using two-dimensional gel protein electrophoresis and mass spectrometry. In adults and fifth-instar nymphs, 127 and 162 protein spots were detected, respectively. Fifty-five differentially expressed protein spots were identified between the long-winged adults and the short-winged adults, and 62 differentially expressed protein spots were found between the long-winged disc fifth-instar nymphs and short-winged disc fifth-instar nymphs. In long-winged and short-winged adults, six and seven specific protein spots were identified, respectively, with five and seven protein spots having more than threefold increased level, respectively. In long-winged and short-winged disc morph nymphs, 8 and 12 specific protein spots were identified, respectively, with 11 and 17 spots containing more than threefold increased level, respectively. Among the 16 identified proteins, five proteins are associated with muscle function, suggesting that muscle is a main tissue where the genes were differentially expressed between the two wing types. In addition, the content of a peptidase with an insulinase domain was higher (by 3.02 ± 0.59 fold) in the short-winged fifth-instar nymphs than in the long-winged fifth-instar nymphs, which suggests that this peptidase may be involved in wing differentiation by regulating insulin receptors. The results of this study provide some genetic clues for the wing differential development in S. furcifera and provide more references for future studies. Oxford University Press 2016-04-04 /pmc/articles/PMC4819851/ /pubmed/27044649 http://dx.doi.org/10.1093/jisesa/iew024 Text en © The Author 2016. Published by Oxford University Press on behalf of the Entomological Society of America. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Liang, Zi-Qiang
Song, Shao-Yun
Liang, Shi-Ke
Wang, Fang-Hai
Analysis of Differential Proteins in Two Wing-Type Females of Sogatella furcifera (Hemiptera: Delphacidae)
title Analysis of Differential Proteins in Two Wing-Type Females of Sogatella furcifera (Hemiptera: Delphacidae)
title_full Analysis of Differential Proteins in Two Wing-Type Females of Sogatella furcifera (Hemiptera: Delphacidae)
title_fullStr Analysis of Differential Proteins in Two Wing-Type Females of Sogatella furcifera (Hemiptera: Delphacidae)
title_full_unstemmed Analysis of Differential Proteins in Two Wing-Type Females of Sogatella furcifera (Hemiptera: Delphacidae)
title_short Analysis of Differential Proteins in Two Wing-Type Females of Sogatella furcifera (Hemiptera: Delphacidae)
title_sort analysis of differential proteins in two wing-type females of sogatella furcifera (hemiptera: delphacidae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819851/
https://www.ncbi.nlm.nih.gov/pubmed/27044649
http://dx.doi.org/10.1093/jisesa/iew024
work_keys_str_mv AT liangziqiang analysisofdifferentialproteinsintwowingtypefemalesofsogatellafurciferahemipteradelphacidae
AT songshaoyun analysisofdifferentialproteinsintwowingtypefemalesofsogatellafurciferahemipteradelphacidae
AT liangshike analysisofdifferentialproteinsintwowingtypefemalesofsogatellafurciferahemipteradelphacidae
AT wangfanghai analysisofdifferentialproteinsintwowingtypefemalesofsogatellafurciferahemipteradelphacidae