Cargando…
A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity
Endothelial Colony Forming Cells (ECFCs), a distinct population of Endothelial Progenitor Cells (EPCs) progeny, display phenotypic and functional characteristics of endothelial cells while retaining features of stem/progenitor cells. Cord blood-derived ECFCs (CB-ECFCs) have a high clonogenic and pro...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820260/ https://www.ncbi.nlm.nih.gov/pubmed/27043207 http://dx.doi.org/10.1371/journal.pone.0152993 |
_version_ | 1782425368809963520 |
---|---|
author | Guillevic, Oriane Ferratge, Ségolène Pascaud, Juliette Driancourt, Catherine Boyer-Di-Ponio, Julie Uzan, Georges |
author_facet | Guillevic, Oriane Ferratge, Ségolène Pascaud, Juliette Driancourt, Catherine Boyer-Di-Ponio, Julie Uzan, Georges |
author_sort | Guillevic, Oriane |
collection | PubMed |
description | Endothelial Colony Forming Cells (ECFCs), a distinct population of Endothelial Progenitor Cells (EPCs) progeny, display phenotypic and functional characteristics of endothelial cells while retaining features of stem/progenitor cells. Cord blood-derived ECFCs (CB-ECFCs) have a high clonogenic and proliferative potentials and they can acquire different endothelial phenotypes, this requiring some plasticity. These properties provide angiogenic and vascular repair capabilities to CB-ECFCs for ischemic cell therapies. However, the degree of immaturity retained by EPCs is still confused and poorly defined. Consequently, to better characterize CB-ECFC stemness, we quantified their clonogenic potential and demonstrated that they were reprogrammed into induced pluripotent stem cells (iPSCs) more efficiently and rapidly than adult endothelial cells. Moreover, we analyzed the transcriptional profile of a broad gene panel known to be related to stem cells. We showed that, unlike mature endothelial cells, CB-ECFCs expressed genes involved in the maintenance of embryonic stem cell properties such as DNMT3B, GDF3 or SOX2. Thus, these results provide further evidence and tools to appreciate EPC-derived cell stemness. Moreover this novel stem cell transcriptional signature of ECFCs could help better characterizing and ranging EPCs according to their immaturity profile. |
format | Online Article Text |
id | pubmed-4820260 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48202602016-04-22 A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity Guillevic, Oriane Ferratge, Ségolène Pascaud, Juliette Driancourt, Catherine Boyer-Di-Ponio, Julie Uzan, Georges PLoS One Research Article Endothelial Colony Forming Cells (ECFCs), a distinct population of Endothelial Progenitor Cells (EPCs) progeny, display phenotypic and functional characteristics of endothelial cells while retaining features of stem/progenitor cells. Cord blood-derived ECFCs (CB-ECFCs) have a high clonogenic and proliferative potentials and they can acquire different endothelial phenotypes, this requiring some plasticity. These properties provide angiogenic and vascular repair capabilities to CB-ECFCs for ischemic cell therapies. However, the degree of immaturity retained by EPCs is still confused and poorly defined. Consequently, to better characterize CB-ECFC stemness, we quantified their clonogenic potential and demonstrated that they were reprogrammed into induced pluripotent stem cells (iPSCs) more efficiently and rapidly than adult endothelial cells. Moreover, we analyzed the transcriptional profile of a broad gene panel known to be related to stem cells. We showed that, unlike mature endothelial cells, CB-ECFCs expressed genes involved in the maintenance of embryonic stem cell properties such as DNMT3B, GDF3 or SOX2. Thus, these results provide further evidence and tools to appreciate EPC-derived cell stemness. Moreover this novel stem cell transcriptional signature of ECFCs could help better characterizing and ranging EPCs according to their immaturity profile. Public Library of Science 2016-04-04 /pmc/articles/PMC4820260/ /pubmed/27043207 http://dx.doi.org/10.1371/journal.pone.0152993 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Guillevic, Oriane Ferratge, Ségolène Pascaud, Juliette Driancourt, Catherine Boyer-Di-Ponio, Julie Uzan, Georges A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity |
title | A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity |
title_full | A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity |
title_fullStr | A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity |
title_full_unstemmed | A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity |
title_short | A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity |
title_sort | novel molecular and functional stemness signature assessing human cord blood-derived endothelial progenitor cell immaturity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820260/ https://www.ncbi.nlm.nih.gov/pubmed/27043207 http://dx.doi.org/10.1371/journal.pone.0152993 |
work_keys_str_mv | AT guillevicoriane anovelmolecularandfunctionalstemnesssignatureassessinghumancordbloodderivedendothelialprogenitorcellimmaturity AT ferratgesegolene anovelmolecularandfunctionalstemnesssignatureassessinghumancordbloodderivedendothelialprogenitorcellimmaturity AT pascaudjuliette anovelmolecularandfunctionalstemnesssignatureassessinghumancordbloodderivedendothelialprogenitorcellimmaturity AT driancourtcatherine anovelmolecularandfunctionalstemnesssignatureassessinghumancordbloodderivedendothelialprogenitorcellimmaturity AT boyerdiponiojulie anovelmolecularandfunctionalstemnesssignatureassessinghumancordbloodderivedendothelialprogenitorcellimmaturity AT uzangeorges anovelmolecularandfunctionalstemnesssignatureassessinghumancordbloodderivedendothelialprogenitorcellimmaturity AT guillevicoriane novelmolecularandfunctionalstemnesssignatureassessinghumancordbloodderivedendothelialprogenitorcellimmaturity AT ferratgesegolene novelmolecularandfunctionalstemnesssignatureassessinghumancordbloodderivedendothelialprogenitorcellimmaturity AT pascaudjuliette novelmolecularandfunctionalstemnesssignatureassessinghumancordbloodderivedendothelialprogenitorcellimmaturity AT driancourtcatherine novelmolecularandfunctionalstemnesssignatureassessinghumancordbloodderivedendothelialprogenitorcellimmaturity AT boyerdiponiojulie novelmolecularandfunctionalstemnesssignatureassessinghumancordbloodderivedendothelialprogenitorcellimmaturity AT uzangeorges novelmolecularandfunctionalstemnesssignatureassessinghumancordbloodderivedendothelialprogenitorcellimmaturity |