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Exopolysaccharides Play a Role in the Swarming of the Benthic Bacterium Pseudoalteromonas sp. SM9913

Most marine bacteria secrete exopolysaccharide (EPS), which is important for bacterial survival in the marine environment. However, it is still unclear whether the self-secreted EPS is involved in marine bacterial motility. Here we studied the role of EPS in the lateral flagella-driven swarming moti...

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Autores principales: Liu, Ang, Mi, Zi-Hao, Zheng, Xiao-Yu, Yu, Yang, Su, Hai-Nan, Chen, Xiu-Lan, Xie, Bin-Bin, Zhou, Bai-Cheng, Zhang, Yu-Zhong, Qin, Qi-Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820436/
https://www.ncbi.nlm.nih.gov/pubmed/27092127
http://dx.doi.org/10.3389/fmicb.2016.00473
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author Liu, Ang
Mi, Zi-Hao
Zheng, Xiao-Yu
Yu, Yang
Su, Hai-Nan
Chen, Xiu-Lan
Xie, Bin-Bin
Zhou, Bai-Cheng
Zhang, Yu-Zhong
Qin, Qi-Long
author_facet Liu, Ang
Mi, Zi-Hao
Zheng, Xiao-Yu
Yu, Yang
Su, Hai-Nan
Chen, Xiu-Lan
Xie, Bin-Bin
Zhou, Bai-Cheng
Zhang, Yu-Zhong
Qin, Qi-Long
author_sort Liu, Ang
collection PubMed
description Most marine bacteria secrete exopolysaccharide (EPS), which is important for bacterial survival in the marine environment. However, it is still unclear whether the self-secreted EPS is involved in marine bacterial motility. Here we studied the role of EPS in the lateral flagella-driven swarming motility of benthic bacterium Pseudoalteromonas sp. SM9913 (SM9913) by a comparison of wild SM9913 and ΔepsT, an EPS synthesis defective mutant. Reduction of EPS production in ΔepsT did not affect the growth rate or the swimming motility, but significantly decreased the swarming motility on a swarming plate, suggesting that the EPS may play a role in SM9913 swarming. However, the expression and assembly of lateral flagella in ΔepsT were not affected. Instead, ΔepsT had a different swarming behavior from wild SM9913. The swarming of ΔepsT did not have an obvious rapid swarming period, and its rate became much lower than that of wild SM9913 after 35 h incubation. An addition of surfactin or SM9913 EPS on the surface of the swarming plate could rescue the swarming level. These results indicate that the self-secreted EPS is required for the swarming of SM9913. This study widens our understanding of the function of the EPS of benthic bacteria.
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spelling pubmed-48204362016-04-18 Exopolysaccharides Play a Role in the Swarming of the Benthic Bacterium Pseudoalteromonas sp. SM9913 Liu, Ang Mi, Zi-Hao Zheng, Xiao-Yu Yu, Yang Su, Hai-Nan Chen, Xiu-Lan Xie, Bin-Bin Zhou, Bai-Cheng Zhang, Yu-Zhong Qin, Qi-Long Front Microbiol Microbiology Most marine bacteria secrete exopolysaccharide (EPS), which is important for bacterial survival in the marine environment. However, it is still unclear whether the self-secreted EPS is involved in marine bacterial motility. Here we studied the role of EPS in the lateral flagella-driven swarming motility of benthic bacterium Pseudoalteromonas sp. SM9913 (SM9913) by a comparison of wild SM9913 and ΔepsT, an EPS synthesis defective mutant. Reduction of EPS production in ΔepsT did not affect the growth rate or the swimming motility, but significantly decreased the swarming motility on a swarming plate, suggesting that the EPS may play a role in SM9913 swarming. However, the expression and assembly of lateral flagella in ΔepsT were not affected. Instead, ΔepsT had a different swarming behavior from wild SM9913. The swarming of ΔepsT did not have an obvious rapid swarming period, and its rate became much lower than that of wild SM9913 after 35 h incubation. An addition of surfactin or SM9913 EPS on the surface of the swarming plate could rescue the swarming level. These results indicate that the self-secreted EPS is required for the swarming of SM9913. This study widens our understanding of the function of the EPS of benthic bacteria. Frontiers Media S.A. 2016-04-05 /pmc/articles/PMC4820436/ /pubmed/27092127 http://dx.doi.org/10.3389/fmicb.2016.00473 Text en Copyright © 2016 Liu, Mi, Zheng, Yu, Su, Chen, Xie, Zhou, Zhang and Qin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Ang
Mi, Zi-Hao
Zheng, Xiao-Yu
Yu, Yang
Su, Hai-Nan
Chen, Xiu-Lan
Xie, Bin-Bin
Zhou, Bai-Cheng
Zhang, Yu-Zhong
Qin, Qi-Long
Exopolysaccharides Play a Role in the Swarming of the Benthic Bacterium Pseudoalteromonas sp. SM9913
title Exopolysaccharides Play a Role in the Swarming of the Benthic Bacterium Pseudoalteromonas sp. SM9913
title_full Exopolysaccharides Play a Role in the Swarming of the Benthic Bacterium Pseudoalteromonas sp. SM9913
title_fullStr Exopolysaccharides Play a Role in the Swarming of the Benthic Bacterium Pseudoalteromonas sp. SM9913
title_full_unstemmed Exopolysaccharides Play a Role in the Swarming of the Benthic Bacterium Pseudoalteromonas sp. SM9913
title_short Exopolysaccharides Play a Role in the Swarming of the Benthic Bacterium Pseudoalteromonas sp. SM9913
title_sort exopolysaccharides play a role in the swarming of the benthic bacterium pseudoalteromonas sp. sm9913
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820436/
https://www.ncbi.nlm.nih.gov/pubmed/27092127
http://dx.doi.org/10.3389/fmicb.2016.00473
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