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Prevalence of β-lactam (bla(TEM)) and Metronidazole (nim) Resistance Genes in the Oral Cavity of Greek Subjects

OBJECTIVES: The aim of this study is to investigate the prevalence of bla(TEM) and nim genes that encode resistance to β-lactams and nitroimidazoles, respectively, in the oral cavity of systemically healthy Greek subjects. MATERIALS AND METHODOLOGY: After screening 720 potentially eligible subjects,...

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Autores principales: Koukos, Georgios, Konstantinidis, Antonios, Tsalikis, Lazaros, Arsenakis, Minas, Slini, Theodora, Sakellari, Dimitra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820533/
https://www.ncbi.nlm.nih.gov/pubmed/27099637
http://dx.doi.org/10.2174/1874210601610010089
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author Koukos, Georgios
Konstantinidis, Antonios
Tsalikis, Lazaros
Arsenakis, Minas
Slini, Theodora
Sakellari, Dimitra
author_facet Koukos, Georgios
Konstantinidis, Antonios
Tsalikis, Lazaros
Arsenakis, Minas
Slini, Theodora
Sakellari, Dimitra
author_sort Koukos, Georgios
collection PubMed
description OBJECTIVES: The aim of this study is to investigate the prevalence of bla(TEM) and nim genes that encode resistance to β-lactams and nitroimidazoles, respectively, in the oral cavity of systemically healthy Greek subjects. MATERIALS AND METHODOLOGY: After screening 720 potentially eligible subjects, 154 subjects were recruited for the study, including 50 periodontally healthy patients, 52 cases of gingivitis and 52 cases of chronic periodontitis. The clinical parameters were assessed with an automated probe. Various samples were collected from the tongue, first molars and pockets >6mm, and analysed by polymerase chain reaction-amplification of the bla(TEM) and nim genes, using primers and conditions previously described in the literature. RESULTS: There was a high rate of detection of bla(TEM) in plaque and tongue samples alike in all periodontal conditions (37% of plaque and 60% of tongue samples, and 71% of participants). The bla(TEM) gene was detected more frequently in the tongue samples of the periodontally healthy (56%) and chronic periodontitis (62%) groups compared to the plaque samples from the same groups (36% and 29%, respectively; z-test with Bonferroni corrections-tests, P<0.05). The nim gene was not detected in any of the 343 samples analysed. CONCLUSION: The oral cavity of Greek subjects often harbours bla(TEM) but not nim genes, and therefore the antimicrobial activity of β-lactams might be compromised.
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spelling pubmed-48205332016-04-20 Prevalence of β-lactam (bla(TEM)) and Metronidazole (nim) Resistance Genes in the Oral Cavity of Greek Subjects Koukos, Georgios Konstantinidis, Antonios Tsalikis, Lazaros Arsenakis, Minas Slini, Theodora Sakellari, Dimitra Open Dent J Article OBJECTIVES: The aim of this study is to investigate the prevalence of bla(TEM) and nim genes that encode resistance to β-lactams and nitroimidazoles, respectively, in the oral cavity of systemically healthy Greek subjects. MATERIALS AND METHODOLOGY: After screening 720 potentially eligible subjects, 154 subjects were recruited for the study, including 50 periodontally healthy patients, 52 cases of gingivitis and 52 cases of chronic periodontitis. The clinical parameters were assessed with an automated probe. Various samples were collected from the tongue, first molars and pockets >6mm, and analysed by polymerase chain reaction-amplification of the bla(TEM) and nim genes, using primers and conditions previously described in the literature. RESULTS: There was a high rate of detection of bla(TEM) in plaque and tongue samples alike in all periodontal conditions (37% of plaque and 60% of tongue samples, and 71% of participants). The bla(TEM) gene was detected more frequently in the tongue samples of the periodontally healthy (56%) and chronic periodontitis (62%) groups compared to the plaque samples from the same groups (36% and 29%, respectively; z-test with Bonferroni corrections-tests, P<0.05). The nim gene was not detected in any of the 343 samples analysed. CONCLUSION: The oral cavity of Greek subjects often harbours bla(TEM) but not nim genes, and therefore the antimicrobial activity of β-lactams might be compromised. Bentham Open 2016-03-28 /pmc/articles/PMC4820533/ /pubmed/27099637 http://dx.doi.org/10.2174/1874210601610010089 Text en © Koukos et al. ; Licensee Bentham Open. https://creativecommons.org/licenses/by/4.0/legalcode This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Koukos, Georgios
Konstantinidis, Antonios
Tsalikis, Lazaros
Arsenakis, Minas
Slini, Theodora
Sakellari, Dimitra
Prevalence of β-lactam (bla(TEM)) and Metronidazole (nim) Resistance Genes in the Oral Cavity of Greek Subjects
title Prevalence of β-lactam (bla(TEM)) and Metronidazole (nim) Resistance Genes in the Oral Cavity of Greek Subjects
title_full Prevalence of β-lactam (bla(TEM)) and Metronidazole (nim) Resistance Genes in the Oral Cavity of Greek Subjects
title_fullStr Prevalence of β-lactam (bla(TEM)) and Metronidazole (nim) Resistance Genes in the Oral Cavity of Greek Subjects
title_full_unstemmed Prevalence of β-lactam (bla(TEM)) and Metronidazole (nim) Resistance Genes in the Oral Cavity of Greek Subjects
title_short Prevalence of β-lactam (bla(TEM)) and Metronidazole (nim) Resistance Genes in the Oral Cavity of Greek Subjects
title_sort prevalence of β-lactam (bla(tem)) and metronidazole (nim) resistance genes in the oral cavity of greek subjects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820533/
https://www.ncbi.nlm.nih.gov/pubmed/27099637
http://dx.doi.org/10.2174/1874210601610010089
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