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Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic

Less than 20 sporadic cases of human Zika virus (ZIKV) infection were reported in Africa and Asia before 2007, but large outbreaks involving up to 73% of the populations on the Pacific islands have started since 2007, and spread to the Americas in 2014. Moreover, the clinical manifestation of ZIKV i...

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Autores principales: Zhu, Zheng, Chan, Jasper Fuk-Woo, Tee, Kah-Meng, Choi, Garnet Kwan-Yue, Lau, Susanna Kar-Pui, Woo, Patrick Chiu-Yat, Tse, Herman, Yuen, Kwok-Yung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820678/
https://www.ncbi.nlm.nih.gov/pubmed/26980239
http://dx.doi.org/10.1038/emi.2016.48
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author Zhu, Zheng
Chan, Jasper Fuk-Woo
Tee, Kah-Meng
Choi, Garnet Kwan-Yue
Lau, Susanna Kar-Pui
Woo, Patrick Chiu-Yat
Tse, Herman
Yuen, Kwok-Yung
author_facet Zhu, Zheng
Chan, Jasper Fuk-Woo
Tee, Kah-Meng
Choi, Garnet Kwan-Yue
Lau, Susanna Kar-Pui
Woo, Patrick Chiu-Yat
Tse, Herman
Yuen, Kwok-Yung
author_sort Zhu, Zheng
collection PubMed
description Less than 20 sporadic cases of human Zika virus (ZIKV) infection were reported in Africa and Asia before 2007, but large outbreaks involving up to 73% of the populations on the Pacific islands have started since 2007, and spread to the Americas in 2014. Moreover, the clinical manifestation of ZIKV infection has apparently changed, as evident by increasing reports of neurological complications, such as Guillain–Barré syndrome in adults and congenital anomalies in neonates. We comprehensively compared the genome sequences of pre-epidemic and epidemic ZIKV strains with complete genome or complete polyprotein sequences available in GenBank. Besides the reported phylogenetic clustering of the epidemic strains with the Asian lineage, we found that the topology of phylogenetic tree of all coding regions is the same except that of the non-structural 2B (NS2B) coding region. This finding was confirmed by bootscan analysis and multiple sequence alignment, which suggested the presence of a fragment of genetic recombination at NS2B with that of Spondweni virus. Moreover, the representative epidemic strain possesses one large bulge of nine bases instead of an external loop on the first stem-loop structure at the 3′-untranslated region just distal to the stop codon of the NS5 in the 1947 pre-epidemic prototype strain. Fifteen amino acid substitutions are found in the epidemic strains when compared with the pre-epidemic strains. As mutations in other flaviviruses can be associated with changes in virulence, replication efficiency, antigenic epitopes and host tropism, further studies would be important to ascertain the biological significance of these genomic changes.
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spelling pubmed-48206782016-04-17 Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic Zhu, Zheng Chan, Jasper Fuk-Woo Tee, Kah-Meng Choi, Garnet Kwan-Yue Lau, Susanna Kar-Pui Woo, Patrick Chiu-Yat Tse, Herman Yuen, Kwok-Yung Emerg Microbes Infect Original Article Less than 20 sporadic cases of human Zika virus (ZIKV) infection were reported in Africa and Asia before 2007, but large outbreaks involving up to 73% of the populations on the Pacific islands have started since 2007, and spread to the Americas in 2014. Moreover, the clinical manifestation of ZIKV infection has apparently changed, as evident by increasing reports of neurological complications, such as Guillain–Barré syndrome in adults and congenital anomalies in neonates. We comprehensively compared the genome sequences of pre-epidemic and epidemic ZIKV strains with complete genome or complete polyprotein sequences available in GenBank. Besides the reported phylogenetic clustering of the epidemic strains with the Asian lineage, we found that the topology of phylogenetic tree of all coding regions is the same except that of the non-structural 2B (NS2B) coding region. This finding was confirmed by bootscan analysis and multiple sequence alignment, which suggested the presence of a fragment of genetic recombination at NS2B with that of Spondweni virus. Moreover, the representative epidemic strain possesses one large bulge of nine bases instead of an external loop on the first stem-loop structure at the 3′-untranslated region just distal to the stop codon of the NS5 in the 1947 pre-epidemic prototype strain. Fifteen amino acid substitutions are found in the epidemic strains when compared with the pre-epidemic strains. As mutations in other flaviviruses can be associated with changes in virulence, replication efficiency, antigenic epitopes and host tropism, further studies would be important to ascertain the biological significance of these genomic changes. Nature Publishing Group 2016-03 2016-03-16 /pmc/articles/PMC4820678/ /pubmed/26980239 http://dx.doi.org/10.1038/emi.2016.48 Text en Copyright © 2016 Shanghai Shangyixun Cultural Communication Co., Ltd http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Original Article
Zhu, Zheng
Chan, Jasper Fuk-Woo
Tee, Kah-Meng
Choi, Garnet Kwan-Yue
Lau, Susanna Kar-Pui
Woo, Patrick Chiu-Yat
Tse, Herman
Yuen, Kwok-Yung
Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic
title Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic
title_full Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic
title_fullStr Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic
title_full_unstemmed Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic
title_short Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic
title_sort comparative genomic analysis of pre-epidemic and epidemic zika virus strains for virological factors potentially associated with the rapidly expanding epidemic
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820678/
https://www.ncbi.nlm.nih.gov/pubmed/26980239
http://dx.doi.org/10.1038/emi.2016.48
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