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Anti-Leishmania Activity of Osthole

BACKGROUND: Treatment of cutaneous leishmaniasis (CL) is occasionally highly resistant to pentavalent antimonials, the gold standard in pharmacotherapy of CL. Since there is no effective vaccine, the discovery of natural antileishmanial products as complementary therapeutic agents could be used to i...

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Autores principales: Kermani, Elaheh Kordzadeh, Sajjadi, Seyed Ebrahim, Hejazi, Seyed Hossein, Arjmand, Reza, Saberi, Sedigheh, Eskandarian, Abbas Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4821100/
https://www.ncbi.nlm.nih.gov/pubmed/27114685
http://dx.doi.org/10.4103/0974-8490.178650
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author Kermani, Elaheh Kordzadeh
Sajjadi, Seyed Ebrahim
Hejazi, Seyed Hossein
Arjmand, Reza
Saberi, Sedigheh
Eskandarian, Abbas Ali
author_facet Kermani, Elaheh Kordzadeh
Sajjadi, Seyed Ebrahim
Hejazi, Seyed Hossein
Arjmand, Reza
Saberi, Sedigheh
Eskandarian, Abbas Ali
author_sort Kermani, Elaheh Kordzadeh
collection PubMed
description BACKGROUND: Treatment of cutaneous leishmaniasis (CL) is occasionally highly resistant to pentavalent antimonials, the gold standard in pharmacotherapy of CL. Since there is no effective vaccine, the discovery of natural antileishmanial products as complementary therapeutic agents could be used to improve the current regimens. OBJECTIVE: In this study in vitro and in vivo antileishmanial activities of osthole, a natural coumarin known to possess antibacterial and parasiticidal activities are evaluated. MATERIALS AND METHODS: Leishmania major infected J774.A1 macrophages were treated with increasing concentrations of osthole. CL lesions of BALB/c mice were treated topically with 0.2% osthole. RESULTS: Osthole exhibited dose-dependent leishmanicidal activity against intracellular amastigotes with IC(50) value of 14.95 μg/ml. Treatment of CL lesions in BALB/c mice with osthole significantly declined lesion progression compared to untreated mice (P < 0.05), however did not result in recovery. CONCLUSION: Osthole demonstrated remarkable leishmanicidal activity in vitro. Higher concentrations of osthole may demonstrate the therapeutic property in vivo. SUMMARY: In vitro and in vivo antileishmanial activities of osthole, a pernylated coumarin extracted from Prangos asperula Boiss., are studied against Leishmania major. [Image: see text]
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spelling pubmed-48211002016-04-25 Anti-Leishmania Activity of Osthole Kermani, Elaheh Kordzadeh Sajjadi, Seyed Ebrahim Hejazi, Seyed Hossein Arjmand, Reza Saberi, Sedigheh Eskandarian, Abbas Ali Pharmacognosy Res Original Article BACKGROUND: Treatment of cutaneous leishmaniasis (CL) is occasionally highly resistant to pentavalent antimonials, the gold standard in pharmacotherapy of CL. Since there is no effective vaccine, the discovery of natural antileishmanial products as complementary therapeutic agents could be used to improve the current regimens. OBJECTIVE: In this study in vitro and in vivo antileishmanial activities of osthole, a natural coumarin known to possess antibacterial and parasiticidal activities are evaluated. MATERIALS AND METHODS: Leishmania major infected J774.A1 macrophages were treated with increasing concentrations of osthole. CL lesions of BALB/c mice were treated topically with 0.2% osthole. RESULTS: Osthole exhibited dose-dependent leishmanicidal activity against intracellular amastigotes with IC(50) value of 14.95 μg/ml. Treatment of CL lesions in BALB/c mice with osthole significantly declined lesion progression compared to untreated mice (P < 0.05), however did not result in recovery. CONCLUSION: Osthole demonstrated remarkable leishmanicidal activity in vitro. Higher concentrations of osthole may demonstrate the therapeutic property in vivo. SUMMARY: In vitro and in vivo antileishmanial activities of osthole, a pernylated coumarin extracted from Prangos asperula Boiss., are studied against Leishmania major. [Image: see text] Medknow Publications & Media Pvt Ltd 2016-03 /pmc/articles/PMC4821100/ /pubmed/27114685 http://dx.doi.org/10.4103/0974-8490.178650 Text en Copyright: © 2016 Pharmacognosy Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Kermani, Elaheh Kordzadeh
Sajjadi, Seyed Ebrahim
Hejazi, Seyed Hossein
Arjmand, Reza
Saberi, Sedigheh
Eskandarian, Abbas Ali
Anti-Leishmania Activity of Osthole
title Anti-Leishmania Activity of Osthole
title_full Anti-Leishmania Activity of Osthole
title_fullStr Anti-Leishmania Activity of Osthole
title_full_unstemmed Anti-Leishmania Activity of Osthole
title_short Anti-Leishmania Activity of Osthole
title_sort anti-leishmania activity of osthole
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4821100/
https://www.ncbi.nlm.nih.gov/pubmed/27114685
http://dx.doi.org/10.4103/0974-8490.178650
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