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The Role of MicroRNAs in Response to Interferon Treatment of Chronic Hepatitis C patients
INTRODUCTION: Treatment of HCV using a combination of pegylated interferon (PEG-IFN) and ribavirin fails in about 40% of the patients with HCV genotype 4 infections, and it is physically and economically demanding. Thus, it is highly important to identify factors that can help to predict the likelih...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Electronic physician
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4821316/ https://www.ncbi.nlm.nih.gov/pubmed/27054010 http://dx.doi.org/10.19082/1994 |
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author | El-Ahwany, Eman Nagy, Faten Zoheiry, Mona ELGhannam, Maged Shemis, Mohamed Aboul-Ezz, Mohamed Zada, Suher |
author_facet | El-Ahwany, Eman Nagy, Faten Zoheiry, Mona ELGhannam, Maged Shemis, Mohamed Aboul-Ezz, Mohamed Zada, Suher |
author_sort | El-Ahwany, Eman |
collection | PubMed |
description | INTRODUCTION: Treatment of HCV using a combination of pegylated interferon (PEG-IFN) and ribavirin fails in about 40% of the patients with HCV genotype 4 infections, and it is physically and economically demanding. Thus, it is highly important to identify factors that can help to predict the likelihood that a patient will respond to this treatment. METHODS: In this study, five miRNAs, i.e., miRNA-122, miRNA-199, miRNA-192, miRNA-30, and miRNA-128, were selected according to previous studies that demonstrated their noticeable functions in viral replication, indicating that they potentially could be used by host cells to control viral infections. The five miRNAs were measured using real-time, reverse transcription-polymerase chain reactions. The data were analyzed using the t-test and chi-squared test. RESULTS: We found that the expression level of miRNA-122 was significantly increased in the responders’ group (p < 0.01) over that in the non-responders’ groups before and after treatment; both increased significantly (p < 0.01) compared with the normal control group. CONCLUSION: miR-122 might be a useful predictor for virological responses to treatment with PEG-interferon plus ribavirin therapy in patients with HCV. |
format | Online Article Text |
id | pubmed-4821316 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Electronic physician |
record_format | MEDLINE/PubMed |
spelling | pubmed-48213162016-04-06 The Role of MicroRNAs in Response to Interferon Treatment of Chronic Hepatitis C patients El-Ahwany, Eman Nagy, Faten Zoheiry, Mona ELGhannam, Maged Shemis, Mohamed Aboul-Ezz, Mohamed Zada, Suher Electron Physician Original Article INTRODUCTION: Treatment of HCV using a combination of pegylated interferon (PEG-IFN) and ribavirin fails in about 40% of the patients with HCV genotype 4 infections, and it is physically and economically demanding. Thus, it is highly important to identify factors that can help to predict the likelihood that a patient will respond to this treatment. METHODS: In this study, five miRNAs, i.e., miRNA-122, miRNA-199, miRNA-192, miRNA-30, and miRNA-128, were selected according to previous studies that demonstrated their noticeable functions in viral replication, indicating that they potentially could be used by host cells to control viral infections. The five miRNAs were measured using real-time, reverse transcription-polymerase chain reactions. The data were analyzed using the t-test and chi-squared test. RESULTS: We found that the expression level of miRNA-122 was significantly increased in the responders’ group (p < 0.01) over that in the non-responders’ groups before and after treatment; both increased significantly (p < 0.01) compared with the normal control group. CONCLUSION: miR-122 might be a useful predictor for virological responses to treatment with PEG-interferon plus ribavirin therapy in patients with HCV. Electronic physician 2016-02-25 /pmc/articles/PMC4821316/ /pubmed/27054010 http://dx.doi.org/10.19082/1994 Text en © 2016 The Authors This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (http://creativecommons.org/licenses/by-nc-nd/3.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Original Article El-Ahwany, Eman Nagy, Faten Zoheiry, Mona ELGhannam, Maged Shemis, Mohamed Aboul-Ezz, Mohamed Zada, Suher The Role of MicroRNAs in Response to Interferon Treatment of Chronic Hepatitis C patients |
title | The Role of MicroRNAs in Response to Interferon Treatment of Chronic Hepatitis C patients |
title_full | The Role of MicroRNAs in Response to Interferon Treatment of Chronic Hepatitis C patients |
title_fullStr | The Role of MicroRNAs in Response to Interferon Treatment of Chronic Hepatitis C patients |
title_full_unstemmed | The Role of MicroRNAs in Response to Interferon Treatment of Chronic Hepatitis C patients |
title_short | The Role of MicroRNAs in Response to Interferon Treatment of Chronic Hepatitis C patients |
title_sort | role of micrornas in response to interferon treatment of chronic hepatitis c patients |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4821316/ https://www.ncbi.nlm.nih.gov/pubmed/27054010 http://dx.doi.org/10.19082/1994 |
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