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Mutational probing of protein aggregates to design aggregation‐resistant proteins

Characterization of amorphous protein aggregates may offer insights into the process of aggregation. Eleven single amino acid mutants of lipase (LipA of Bacillus subtilis) were subjected to temperature‐induced aggregation, and the resultant aggregates were characterized for recovery of activity in t...

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Detalles Bibliográficos
Autores principales: Kamal, Mohamad Zahid, Kumar, Virender, Satyamurthi, Kundarapu, Das, Kushal Kumar, Rao, Nalam Madhusudhana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4821347/
https://www.ncbi.nlm.nih.gov/pubmed/27239434
http://dx.doi.org/10.1002/2211-5463.12003