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Evaluation of bias associated with high-multiplex, target-specific pre-amplification

We developed a novel PCR-based pre-amplification (PreAmp) technology that can increase the abundance of over 350 target genes one million-fold. To assess potential bias introduced by PreAmp we utilized ERCC RNA reference standards, a model system that quantifies measurement error in RNA analysis. We...

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Detalles Bibliográficos
Autores principales:	Okino, Steven T., Kong, Michelle, Sarras, Haya, Wang, Yan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2015
Materias:	Research Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822213/ https://www.ncbi.nlm.nih.gov/pubmed/27077043 http://dx.doi.org/10.1016/j.bdq.2015.12.001

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