Cargando…
Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle
BACKGROUND: Our previous study had identified the SNP (g.81966377T > C) and indel (g.81966364D > I) located in the promoter of APM1 to have a significant effect on marbling in Hanwoo. APM1 encodes an adipocytokine called adiponectin, which plays a significant role in lipogenesis. The aim of th...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822224/ https://www.ncbi.nlm.nih.gov/pubmed/27054043 http://dx.doi.org/10.1186/s40781-016-0096-2 |
_version_ | 1782425738038738944 |
---|---|
author | Kwon, Anam Srikanth, Krishnamoorthy Lee, Eunjin Kim, Seonkwan Chung, Hoyoung |
author_facet | Kwon, Anam Srikanth, Krishnamoorthy Lee, Eunjin Kim, Seonkwan Chung, Hoyoung |
author_sort | Kwon, Anam |
collection | PubMed |
description | BACKGROUND: Our previous study had identified the SNP (g.81966377T > C) and indel (g.81966364D > I) located in the promoter of APM1 to have a significant effect on marbling in Hanwoo. APM1 encodes an adipocytokine called adiponectin, which plays a significant role in lipogenesis. The aim of this study was to verify and validate the effect of the SNP and indel on marbling and other carcass traits in a large, representative, countrywide population of Hanwoo cattle. The carcass traits measured were marbling (MAR), backfat thickness (BFT), loin eye area (LEA), and carcass weight (CAW). RESULTS: Primers were designed to amplify 346 bp of the genomic segment that contained the targeted SNP (g.81966377) and the indel (g.81966364). After data curation, the genotypes of 8,378 individuals identified using direct sequencing analysis estimated frequencies for C (0.686) and T (0.314) respectively showing genotype frequencies for CC (0.470), CT (0.430) and TT (0.098). The genotypes were significantly associated with MAR, BFT and LEA. The indel had significant effect on marbling (P < .0001) with strong additive genetic effects. The allele frequencies was estimated at (DEL, 0.864) and insertion (INS, 0.136) presenting genotypes of D/D (75.63 %), D/I (21.44 %), and I/I (2.92 %). Significant departure from Hardy-Weinberg equilibrium was not detected for both the SNP and the indel. CONCLUSION: The SNP genotypes showed significant association with MAR, BFT and LEA with strong additive genetic effects, while the indel was significantly associated with MAR. The results confirmed that the variants can be used as a genetic marker for improving marbling in Hanwoo. |
format | Online Article Text |
id | pubmed-4822224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-48222242016-04-06 Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle Kwon, Anam Srikanth, Krishnamoorthy Lee, Eunjin Kim, Seonkwan Chung, Hoyoung J Anim Sci Technol Research BACKGROUND: Our previous study had identified the SNP (g.81966377T > C) and indel (g.81966364D > I) located in the promoter of APM1 to have a significant effect on marbling in Hanwoo. APM1 encodes an adipocytokine called adiponectin, which plays a significant role in lipogenesis. The aim of this study was to verify and validate the effect of the SNP and indel on marbling and other carcass traits in a large, representative, countrywide population of Hanwoo cattle. The carcass traits measured were marbling (MAR), backfat thickness (BFT), loin eye area (LEA), and carcass weight (CAW). RESULTS: Primers were designed to amplify 346 bp of the genomic segment that contained the targeted SNP (g.81966377) and the indel (g.81966364). After data curation, the genotypes of 8,378 individuals identified using direct sequencing analysis estimated frequencies for C (0.686) and T (0.314) respectively showing genotype frequencies for CC (0.470), CT (0.430) and TT (0.098). The genotypes were significantly associated with MAR, BFT and LEA. The indel had significant effect on marbling (P < .0001) with strong additive genetic effects. The allele frequencies was estimated at (DEL, 0.864) and insertion (INS, 0.136) presenting genotypes of D/D (75.63 %), D/I (21.44 %), and I/I (2.92 %). Significant departure from Hardy-Weinberg equilibrium was not detected for both the SNP and the indel. CONCLUSION: The SNP genotypes showed significant association with MAR, BFT and LEA with strong additive genetic effects, while the indel was significantly associated with MAR. The results confirmed that the variants can be used as a genetic marker for improving marbling in Hanwoo. BioMed Central 2016-04-06 /pmc/articles/PMC4822224/ /pubmed/27054043 http://dx.doi.org/10.1186/s40781-016-0096-2 Text en © Kwon et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Kwon, Anam Srikanth, Krishnamoorthy Lee, Eunjin Kim, Seonkwan Chung, Hoyoung Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle |
title | Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle |
title_full | Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle |
title_fullStr | Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle |
title_full_unstemmed | Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle |
title_short | Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle |
title_sort | confirmation of genotypic effects for the bovine apm1 gene on marbling in hanwoo cattle |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822224/ https://www.ncbi.nlm.nih.gov/pubmed/27054043 http://dx.doi.org/10.1186/s40781-016-0096-2 |
work_keys_str_mv | AT kwonanam confirmationofgenotypiceffectsforthebovineapm1geneonmarblinginhanwoocattle AT srikanthkrishnamoorthy confirmationofgenotypiceffectsforthebovineapm1geneonmarblinginhanwoocattle AT leeeunjin confirmationofgenotypiceffectsforthebovineapm1geneonmarblinginhanwoocattle AT kimseonkwan confirmationofgenotypiceffectsforthebovineapm1geneonmarblinginhanwoocattle AT chunghoyoung confirmationofgenotypiceffectsforthebovineapm1geneonmarblinginhanwoocattle |