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Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle

BACKGROUND: Our previous study had identified the SNP (g.81966377T > C) and indel (g.81966364D > I) located in the promoter of APM1 to have a significant effect on marbling in Hanwoo. APM1 encodes an adipocytokine called adiponectin, which plays a significant role in lipogenesis. The aim of th...

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Autores principales: Kwon, Anam, Srikanth, Krishnamoorthy, Lee, Eunjin, Kim, Seonkwan, Chung, Hoyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822224/
https://www.ncbi.nlm.nih.gov/pubmed/27054043
http://dx.doi.org/10.1186/s40781-016-0096-2
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author Kwon, Anam
Srikanth, Krishnamoorthy
Lee, Eunjin
Kim, Seonkwan
Chung, Hoyoung
author_facet Kwon, Anam
Srikanth, Krishnamoorthy
Lee, Eunjin
Kim, Seonkwan
Chung, Hoyoung
author_sort Kwon, Anam
collection PubMed
description BACKGROUND: Our previous study had identified the SNP (g.81966377T > C) and indel (g.81966364D > I) located in the promoter of APM1 to have a significant effect on marbling in Hanwoo. APM1 encodes an adipocytokine called adiponectin, which plays a significant role in lipogenesis. The aim of this study was to verify and validate the effect of the SNP and indel on marbling and other carcass traits in a large, representative, countrywide population of Hanwoo cattle. The carcass traits measured were marbling (MAR), backfat thickness (BFT), loin eye area (LEA), and carcass weight (CAW). RESULTS: Primers were designed to amplify 346 bp of the genomic segment that contained the targeted SNP (g.81966377) and the indel (g.81966364). After data curation, the genotypes of 8,378 individuals identified using direct sequencing analysis estimated frequencies for C (0.686) and T (0.314) respectively showing genotype frequencies for CC (0.470), CT (0.430) and TT (0.098). The genotypes were significantly associated with MAR, BFT and LEA. The indel had significant effect on marbling (P < .0001) with strong additive genetic effects. The allele frequencies was estimated at (DEL, 0.864) and insertion (INS, 0.136) presenting genotypes of D/D (75.63 %), D/I (21.44 %), and I/I (2.92 %). Significant departure from Hardy-Weinberg equilibrium was not detected for both the SNP and the indel. CONCLUSION: The SNP genotypes showed significant association with MAR, BFT and LEA with strong additive genetic effects, while the indel was significantly associated with MAR. The results confirmed that the variants can be used as a genetic marker for improving marbling in Hanwoo.
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spelling pubmed-48222242016-04-06 Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle Kwon, Anam Srikanth, Krishnamoorthy Lee, Eunjin Kim, Seonkwan Chung, Hoyoung J Anim Sci Technol Research BACKGROUND: Our previous study had identified the SNP (g.81966377T > C) and indel (g.81966364D > I) located in the promoter of APM1 to have a significant effect on marbling in Hanwoo. APM1 encodes an adipocytokine called adiponectin, which plays a significant role in lipogenesis. The aim of this study was to verify and validate the effect of the SNP and indel on marbling and other carcass traits in a large, representative, countrywide population of Hanwoo cattle. The carcass traits measured were marbling (MAR), backfat thickness (BFT), loin eye area (LEA), and carcass weight (CAW). RESULTS: Primers were designed to amplify 346 bp of the genomic segment that contained the targeted SNP (g.81966377) and the indel (g.81966364). After data curation, the genotypes of 8,378 individuals identified using direct sequencing analysis estimated frequencies for C (0.686) and T (0.314) respectively showing genotype frequencies for CC (0.470), CT (0.430) and TT (0.098). The genotypes were significantly associated with MAR, BFT and LEA. The indel had significant effect on marbling (P < .0001) with strong additive genetic effects. The allele frequencies was estimated at (DEL, 0.864) and insertion (INS, 0.136) presenting genotypes of D/D (75.63 %), D/I (21.44 %), and I/I (2.92 %). Significant departure from Hardy-Weinberg equilibrium was not detected for both the SNP and the indel. CONCLUSION: The SNP genotypes showed significant association with MAR, BFT and LEA with strong additive genetic effects, while the indel was significantly associated with MAR. The results confirmed that the variants can be used as a genetic marker for improving marbling in Hanwoo. BioMed Central 2016-04-06 /pmc/articles/PMC4822224/ /pubmed/27054043 http://dx.doi.org/10.1186/s40781-016-0096-2 Text en © Kwon et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Kwon, Anam
Srikanth, Krishnamoorthy
Lee, Eunjin
Kim, Seonkwan
Chung, Hoyoung
Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle
title Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle
title_full Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle
title_fullStr Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle
title_full_unstemmed Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle
title_short Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle
title_sort confirmation of genotypic effects for the bovine apm1 gene on marbling in hanwoo cattle
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822224/
https://www.ncbi.nlm.nih.gov/pubmed/27054043
http://dx.doi.org/10.1186/s40781-016-0096-2
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