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Azole resistance in Candida spp. isolated from Catú Lake, Ceará, Brazil: an efflux-pump-mediated mechanism

Since, there is no study reporting the mechanism of azole resistance among yeasts isolated from aquatic environments; the present study aims to investigate the occurrence of antifungal resistance among yeasts isolated from an aquatic environment, and assess the efflux-pump activity of the azole-resi...

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Autores principales: Brilhante, Raimunda S.N., Paiva, Manoel A.N., Sampaio, Célia M.S., Castelo-Branco, Débora S.C.M., Teixeira, Carlos E.C., de Alencar, Lucas P., Bandeira, Tereza J.P.G., Monteiro, André J., Cordeiro, Rossana A., Pereira-Neto, Waldemiro A., Sidrim, José J.C., Moreira, José L.B., Rocha, Marcos F.G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822745/
https://www.ncbi.nlm.nih.gov/pubmed/26887224
http://dx.doi.org/10.1016/j.bjm.2015.11.008
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author Brilhante, Raimunda S.N.
Paiva, Manoel A.N.
Sampaio, Célia M.S.
Castelo-Branco, Débora S.C.M.
Teixeira, Carlos E.C.
de Alencar, Lucas P.
Bandeira, Tereza J.P.G.
Monteiro, André J.
Cordeiro, Rossana A.
Pereira-Neto, Waldemiro A.
Sidrim, José J.C.
Moreira, José L.B.
Rocha, Marcos F.G.
author_facet Brilhante, Raimunda S.N.
Paiva, Manoel A.N.
Sampaio, Célia M.S.
Castelo-Branco, Débora S.C.M.
Teixeira, Carlos E.C.
de Alencar, Lucas P.
Bandeira, Tereza J.P.G.
Monteiro, André J.
Cordeiro, Rossana A.
Pereira-Neto, Waldemiro A.
Sidrim, José J.C.
Moreira, José L.B.
Rocha, Marcos F.G.
author_sort Brilhante, Raimunda S.N.
collection PubMed
description Since, there is no study reporting the mechanism of azole resistance among yeasts isolated from aquatic environments; the present study aims to investigate the occurrence of antifungal resistance among yeasts isolated from an aquatic environment, and assess the efflux-pump activity of the azole-resistant strains to better understand the mechanism of resistance for this group of drugs. For this purpose, monthly water and sediment samples were collected from Catú Lake, Ceará, Brazil, from March 2011 to February 2012. The obtained yeasts were identified based on morphological and biochemical characteristics. Of the 46 isolates, 37 were Candida spp., 4 were Trichosporon asahii, 3 were Cryptococcus laurentii, 1 Rhodotorula mucilaginosa, and 1 was Kodamaea ohmeri. These isolates were subjected to broth microdilution assay with amphotericin B, itraconazole, and fluconazole, according to the methodology standardized by the Clinical and Laboratory Standards Institute (CLSI). The minimum inhibitory concentrations (MICs) of amphotericin B, itraconazole, and fluconazole were 0.03125–2 μg/mL, 0.0625 to ≥16 μg/mL, and 0.5 to ≥64 μg/mL, respectively, and 13 resistant azole-resistant Candida isolates were detected. A reduction in the azole MICs leading to the phenotypical reversal of the azole resistance was observed upon addition of efflux-pump inhibitors. These findings suggest that the azole resistance among environmental Candida spp. is most likely associated with the overexpression of efflux-pumps.
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spelling pubmed-48227452016-05-17 Azole resistance in Candida spp. isolated from Catú Lake, Ceará, Brazil: an efflux-pump-mediated mechanism Brilhante, Raimunda S.N. Paiva, Manoel A.N. Sampaio, Célia M.S. Castelo-Branco, Débora S.C.M. Teixeira, Carlos E.C. de Alencar, Lucas P. Bandeira, Tereza J.P.G. Monteiro, André J. Cordeiro, Rossana A. Pereira-Neto, Waldemiro A. Sidrim, José J.C. Moreira, José L.B. Rocha, Marcos F.G. Braz J Microbiol Environmental Microbiology Since, there is no study reporting the mechanism of azole resistance among yeasts isolated from aquatic environments; the present study aims to investigate the occurrence of antifungal resistance among yeasts isolated from an aquatic environment, and assess the efflux-pump activity of the azole-resistant strains to better understand the mechanism of resistance for this group of drugs. For this purpose, monthly water and sediment samples were collected from Catú Lake, Ceará, Brazil, from March 2011 to February 2012. The obtained yeasts were identified based on morphological and biochemical characteristics. Of the 46 isolates, 37 were Candida spp., 4 were Trichosporon asahii, 3 were Cryptococcus laurentii, 1 Rhodotorula mucilaginosa, and 1 was Kodamaea ohmeri. These isolates were subjected to broth microdilution assay with amphotericin B, itraconazole, and fluconazole, according to the methodology standardized by the Clinical and Laboratory Standards Institute (CLSI). The minimum inhibitory concentrations (MICs) of amphotericin B, itraconazole, and fluconazole were 0.03125–2 μg/mL, 0.0625 to ≥16 μg/mL, and 0.5 to ≥64 μg/mL, respectively, and 13 resistant azole-resistant Candida isolates were detected. A reduction in the azole MICs leading to the phenotypical reversal of the azole resistance was observed upon addition of efflux-pump inhibitors. These findings suggest that the azole resistance among environmental Candida spp. is most likely associated with the overexpression of efflux-pumps. Elsevier 2016-02-17 /pmc/articles/PMC4822745/ /pubmed/26887224 http://dx.doi.org/10.1016/j.bjm.2015.11.008 Text en © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Environmental Microbiology
Brilhante, Raimunda S.N.
Paiva, Manoel A.N.
Sampaio, Célia M.S.
Castelo-Branco, Débora S.C.M.
Teixeira, Carlos E.C.
de Alencar, Lucas P.
Bandeira, Tereza J.P.G.
Monteiro, André J.
Cordeiro, Rossana A.
Pereira-Neto, Waldemiro A.
Sidrim, José J.C.
Moreira, José L.B.
Rocha, Marcos F.G.
Azole resistance in Candida spp. isolated from Catú Lake, Ceará, Brazil: an efflux-pump-mediated mechanism
title Azole resistance in Candida spp. isolated from Catú Lake, Ceará, Brazil: an efflux-pump-mediated mechanism
title_full Azole resistance in Candida spp. isolated from Catú Lake, Ceará, Brazil: an efflux-pump-mediated mechanism
title_fullStr Azole resistance in Candida spp. isolated from Catú Lake, Ceará, Brazil: an efflux-pump-mediated mechanism
title_full_unstemmed Azole resistance in Candida spp. isolated from Catú Lake, Ceará, Brazil: an efflux-pump-mediated mechanism
title_short Azole resistance in Candida spp. isolated from Catú Lake, Ceará, Brazil: an efflux-pump-mediated mechanism
title_sort azole resistance in candida spp. isolated from catú lake, ceará, brazil: an efflux-pump-mediated mechanism
topic Environmental Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822745/
https://www.ncbi.nlm.nih.gov/pubmed/26887224
http://dx.doi.org/10.1016/j.bjm.2015.11.008
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