Alternariol 9-methyl ether from the endophytic fungus Alternaria sp. Samif01 and its bioactivities

One bioactive compound, identified as alternariol 9-methyl ether, was isolated from the crude extract of the endophytic fungus Alternaria sp. Samif01 residing in the roots of Salvia miltiorrhiza Bunge. Alternariol 9-methyl ether was active against bacteria with minimum inhibitory concentration values ranging from 25 to 75 μg/mL and median inhibitory concentration (IC(50)) values ranging from 16.00 to 38.27 μg/mL. The IC(50) value of alternariol 9-methyl ether against spore germination of Magnaporthe oryzae was 87.18 μg/mL. Alternariol 9-methyl ether also showed antinematodal activity against Bursaphelenchus xylophilus and Caenorhabditis elegans with IC(50) values of 98.17 μg/mL and 74.62 μg/mL, respectively. This work is the first report on alternariol 9-methyl ether and its biological activities from the endophytic fungus Alternaria sp. Samif01 derived from S. miltiorrhiza Bunge. The results indicate the potential of Alternaria sp. Samif01 as a source of alternariol 9-methyl ether and also support that alternariol 9-methyl ether is a natural compound with high potential bioactivity against microorganisms.