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Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity
REIC/DKK-3 is a tumor suppressor, however, its intracellular physiological functions and interacting molecules have not been fully clarified. Using yeast two-hybrid screening, we found that small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA), known as a negative modulator of cy...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823106/ https://www.ncbi.nlm.nih.gov/pubmed/26658102 http://dx.doi.org/10.18632/oncotarget.6488 |
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author | Ochiai, Kazuhiko Morimatsu, Masami Kato, Yuiko Ishiguro-Oonuma, Toshina Udagawa, Chihiro Rungsuriyawiboon, Oumaporn Azakami, Daigo Michishita, Masaki Ariyoshi, Yuichi Ueki, Hideo Nasu, Yasutomo Kumon, Hiromi Watanabe, Masami Omi, Toshinori |
author_facet | Ochiai, Kazuhiko Morimatsu, Masami Kato, Yuiko Ishiguro-Oonuma, Toshina Udagawa, Chihiro Rungsuriyawiboon, Oumaporn Azakami, Daigo Michishita, Masaki Ariyoshi, Yuichi Ueki, Hideo Nasu, Yasutomo Kumon, Hiromi Watanabe, Masami Omi, Toshinori |
author_sort | Ochiai, Kazuhiko |
collection | PubMed |
description | REIC/DKK-3 is a tumor suppressor, however, its intracellular physiological functions and interacting molecules have not been fully clarified. Using yeast two-hybrid screening, we found that small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA), known as a negative modulator of cytoplasmic androgen receptor (AR) signaling, is a novel interacting partner of REIC/DKK-3. Mammalian two-hybrid and pull-down assay results indicated that the SGTA-REIC/DKK-3 interaction involved the N-terminal regions of both REIC/DKK-3 and SGTA and that REIC/DKK-3 interfered with the dimerization of SGTA, which is a component of the AR complex and a suppressor of dynein motor-dependent AR transport and signaling. A reporter assay in human prostate cancer cells that displayed suppressed AR signaling by SGTA showed recovery of AR signaling by REIC/DKK-3 expression. Considering these results and our previous data that REIC/DKK-3 interacts with the dynein light chain TCTEX-1, we propose that the REIC/DKK-3 protein interferes with SGTA dimerization, promotes dynein-dependent AR transport and then upregulates AR signaling. |
format | Online Article Text |
id | pubmed-4823106 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-48231062016-05-03 Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity Ochiai, Kazuhiko Morimatsu, Masami Kato, Yuiko Ishiguro-Oonuma, Toshina Udagawa, Chihiro Rungsuriyawiboon, Oumaporn Azakami, Daigo Michishita, Masaki Ariyoshi, Yuichi Ueki, Hideo Nasu, Yasutomo Kumon, Hiromi Watanabe, Masami Omi, Toshinori Oncotarget Research Paper REIC/DKK-3 is a tumor suppressor, however, its intracellular physiological functions and interacting molecules have not been fully clarified. Using yeast two-hybrid screening, we found that small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA), known as a negative modulator of cytoplasmic androgen receptor (AR) signaling, is a novel interacting partner of REIC/DKK-3. Mammalian two-hybrid and pull-down assay results indicated that the SGTA-REIC/DKK-3 interaction involved the N-terminal regions of both REIC/DKK-3 and SGTA and that REIC/DKK-3 interfered with the dimerization of SGTA, which is a component of the AR complex and a suppressor of dynein motor-dependent AR transport and signaling. A reporter assay in human prostate cancer cells that displayed suppressed AR signaling by SGTA showed recovery of AR signaling by REIC/DKK-3 expression. Considering these results and our previous data that REIC/DKK-3 interacts with the dynein light chain TCTEX-1, we propose that the REIC/DKK-3 protein interferes with SGTA dimerization, promotes dynein-dependent AR transport and then upregulates AR signaling. Impact Journals LLC 2015-12-02 /pmc/articles/PMC4823106/ /pubmed/26658102 http://dx.doi.org/10.18632/oncotarget.6488 Text en Copyright: © 2016 Ochiai et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Ochiai, Kazuhiko Morimatsu, Masami Kato, Yuiko Ishiguro-Oonuma, Toshina Udagawa, Chihiro Rungsuriyawiboon, Oumaporn Azakami, Daigo Michishita, Masaki Ariyoshi, Yuichi Ueki, Hideo Nasu, Yasutomo Kumon, Hiromi Watanabe, Masami Omi, Toshinori Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity |
title | Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity |
title_full | Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity |
title_fullStr | Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity |
title_full_unstemmed | Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity |
title_short | Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity |
title_sort | tumor suppressor reic/dkk-3 and co-chaperone sgta: their interaction and roles in the androgen sensitivity |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823106/ https://www.ncbi.nlm.nih.gov/pubmed/26658102 http://dx.doi.org/10.18632/oncotarget.6488 |
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