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FOXP1 functions as an oncogene in promoting cancer stem cell-like characteristics in ovarian cancer cells
Ovarian cancer has the highest mortality rate of all gynecological cancers with a high recurrence rate. It is important to understand the nature of recurring cancer cells to terminally eliminate ovarian cancer. The winged helix transcription factor Forkhead box P1 (FOXP1) has been reported to functi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823123/ https://www.ncbi.nlm.nih.gov/pubmed/26654944 http://dx.doi.org/10.18632/oncotarget.6510 |
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author | Choi, Eun Jung Seo, Eun Jin Kim, Dae Kyoung Lee, Su In Kwon, Yang Woo Jang, Il Ho Kim, Ki-Hyung Suh, Dong-Soo Kim, Jae Ho |
author_facet | Choi, Eun Jung Seo, Eun Jin Kim, Dae Kyoung Lee, Su In Kwon, Yang Woo Jang, Il Ho Kim, Ki-Hyung Suh, Dong-Soo Kim, Jae Ho |
author_sort | Choi, Eun Jung |
collection | PubMed |
description | Ovarian cancer has the highest mortality rate of all gynecological cancers with a high recurrence rate. It is important to understand the nature of recurring cancer cells to terminally eliminate ovarian cancer. The winged helix transcription factor Forkhead box P1 (FOXP1) has been reported to function as either oncogene or tumor-suppressor in various cancers. In the current study, we show that FOXP1 promotes cancer stem cell-like characteristics in ovarian cancer cells. Knockdown of FOXP1 expression in A2780 or SKOV3 ovarian cancer cells decreased spheroid formation, expression of stemness-related genes and epithelial to mesenchymal transition-related genes, cell migration, and resistance to Paclitaxel or Cisplatin treatment, whereas overexpression of FOXP1 in A2780 or SKOV3 ovarian cancer cells increased spheroid formation, expression of stemness-related genes and epithelial to mesenchymal transition-related genes, cell migration, and resistance to Paclitaxel or Cisplatin treatment. In addition, overexpression of FOXP1 increased promoter activity of ABCG2, OCT4, NANOG, and SOX2, among which the increases in ABCG2, OCT4, and SOX2 promoter activity were dependent on the presence of FOXP1-binding site. In xenotransplantation of A2780 ovarian cancer cells into nude mice, knockdown of FOXP1 expression significantly decreased tumor size. These results strongly suggest FOXP1 functions as an oncogene by promoting cancer stem cell-like characteristics in ovarian cancer cells. Targeting FOXP1 may provide a novel therapeutic opportunity for developing a relapse-free treatment for ovarian cancer patients. |
format | Online Article Text |
id | pubmed-4823123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-48231232016-05-03 FOXP1 functions as an oncogene in promoting cancer stem cell-like characteristics in ovarian cancer cells Choi, Eun Jung Seo, Eun Jin Kim, Dae Kyoung Lee, Su In Kwon, Yang Woo Jang, Il Ho Kim, Ki-Hyung Suh, Dong-Soo Kim, Jae Ho Oncotarget Research Paper Ovarian cancer has the highest mortality rate of all gynecological cancers with a high recurrence rate. It is important to understand the nature of recurring cancer cells to terminally eliminate ovarian cancer. The winged helix transcription factor Forkhead box P1 (FOXP1) has been reported to function as either oncogene or tumor-suppressor in various cancers. In the current study, we show that FOXP1 promotes cancer stem cell-like characteristics in ovarian cancer cells. Knockdown of FOXP1 expression in A2780 or SKOV3 ovarian cancer cells decreased spheroid formation, expression of stemness-related genes and epithelial to mesenchymal transition-related genes, cell migration, and resistance to Paclitaxel or Cisplatin treatment, whereas overexpression of FOXP1 in A2780 or SKOV3 ovarian cancer cells increased spheroid formation, expression of stemness-related genes and epithelial to mesenchymal transition-related genes, cell migration, and resistance to Paclitaxel or Cisplatin treatment. In addition, overexpression of FOXP1 increased promoter activity of ABCG2, OCT4, NANOG, and SOX2, among which the increases in ABCG2, OCT4, and SOX2 promoter activity were dependent on the presence of FOXP1-binding site. In xenotransplantation of A2780 ovarian cancer cells into nude mice, knockdown of FOXP1 expression significantly decreased tumor size. These results strongly suggest FOXP1 functions as an oncogene by promoting cancer stem cell-like characteristics in ovarian cancer cells. Targeting FOXP1 may provide a novel therapeutic opportunity for developing a relapse-free treatment for ovarian cancer patients. Impact Journals LLC 2015-12-09 /pmc/articles/PMC4823123/ /pubmed/26654944 http://dx.doi.org/10.18632/oncotarget.6510 Text en Copyright: © 2016 Choi et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Choi, Eun Jung Seo, Eun Jin Kim, Dae Kyoung Lee, Su In Kwon, Yang Woo Jang, Il Ho Kim, Ki-Hyung Suh, Dong-Soo Kim, Jae Ho FOXP1 functions as an oncogene in promoting cancer stem cell-like characteristics in ovarian cancer cells |
title | FOXP1 functions as an oncogene in promoting cancer stem cell-like characteristics in ovarian cancer cells |
title_full | FOXP1 functions as an oncogene in promoting cancer stem cell-like characteristics in ovarian cancer cells |
title_fullStr | FOXP1 functions as an oncogene in promoting cancer stem cell-like characteristics in ovarian cancer cells |
title_full_unstemmed | FOXP1 functions as an oncogene in promoting cancer stem cell-like characteristics in ovarian cancer cells |
title_short | FOXP1 functions as an oncogene in promoting cancer stem cell-like characteristics in ovarian cancer cells |
title_sort | foxp1 functions as an oncogene in promoting cancer stem cell-like characteristics in ovarian cancer cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823123/ https://www.ncbi.nlm.nih.gov/pubmed/26654944 http://dx.doi.org/10.18632/oncotarget.6510 |
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