Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene

The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation a...

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Detalles Bibliográficos
Autores principales: Hewitson, Heema, Wood, Victoria, Kadeva, Neli, Cornwell, Glenda, Codognotto, Stefano, Stephenson, Emma, Ilic, Dusko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Lab Resource: Stem Cell Line
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823668/
https://www.ncbi.nlm.nih.gov/pubmed/27345970
http://dx.doi.org/10.1016/j.scr.2016.01.007
Descripción
Sumario:The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

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