Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene

The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation a...

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Autores principales: Hewitson, Heema, Wood, Victoria, Kadeva, Neli, Cornwell, Glenda, Codognotto, Stefano, Stephenson, Emma, Ilic, Dusko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Lab Resource: Stem Cell Line
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823668/
https://www.ncbi.nlm.nih.gov/pubmed/27345970
http://dx.doi.org/10.1016/j.scr.2016.01.007
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author Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_facet Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_sort Hewitson, Heema
collection PubMed
description The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.
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spelling pubmed-48236682016-04-15 Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene Hewitson, Heema Wood, Victoria Kadeva, Neli Cornwell, Glenda Codognotto, Stefano Stephenson, Emma Ilic, Dusko Stem Cell Res Lab Resource: Stem Cell Line The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays. Elsevier 2016-03 /pmc/articles/PMC4823668/ /pubmed/27345970 http://dx.doi.org/10.1016/j.scr.2016.01.007 Text en © 2016 University of Texas at Austin Dell Medical School http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Lab Resource: Stem Cell Line
Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene
title Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene
title_full Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene
title_fullStr Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene
title_full_unstemmed Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene
title_short Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene
title_sort generation of kcl035 research grade human embryonic stem cell line carrying a mutation in hbb gene
topic Lab Resource: Stem Cell Line
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823668/
https://www.ncbi.nlm.nih.gov/pubmed/27345970
http://dx.doi.org/10.1016/j.scr.2016.01.007
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