Generation of KCL033 clinical grade human embryonic stem cell line

The KCL033 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free env...

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Autores principales: Devito, Liani, Petrova, Anastasia, Wood, Victoria, Kadeva, Neli, Cornwell, Glenda, Codognotto, Stefano, Stephenson, Emma, Ilic, Dusko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Lab Resource: Stem Cell Line
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823760/
https://www.ncbi.nlm.nih.gov/pubmed/27345988
http://dx.doi.org/10.1016/j.scr.2015.12.047
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author Devito, Liani
Petrova, Anastasia
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_facet Devito, Liani
Petrova, Anastasia
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_sort Devito, Liani
collection PubMed
description The KCL033 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility and specific and non-specific human pathogens.
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spelling pubmed-48237602016-04-15 Generation of KCL033 clinical grade human embryonic stem cell line Devito, Liani Petrova, Anastasia Wood, Victoria Kadeva, Neli Cornwell, Glenda Codognotto, Stefano Stephenson, Emma Ilic, Dusko Stem Cell Res Lab Resource: Stem Cell Line The KCL033 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility and specific and non-specific human pathogens. Elsevier 2016-03 /pmc/articles/PMC4823760/ /pubmed/27345988 http://dx.doi.org/10.1016/j.scr.2015.12.047 Text en © 2016 University of Texas at Austin Dell Medical School http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Lab Resource: Stem Cell Line
Devito, Liani
Petrova, Anastasia
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
Generation of KCL033 clinical grade human embryonic stem cell line
title Generation of KCL033 clinical grade human embryonic stem cell line
title_full Generation of KCL033 clinical grade human embryonic stem cell line
title_fullStr Generation of KCL033 clinical grade human embryonic stem cell line
title_full_unstemmed Generation of KCL033 clinical grade human embryonic stem cell line
title_short Generation of KCL033 clinical grade human embryonic stem cell line
title_sort generation of kcl033 clinical grade human embryonic stem cell line
topic Lab Resource: Stem Cell Line
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823760/
https://www.ncbi.nlm.nih.gov/pubmed/27345988
http://dx.doi.org/10.1016/j.scr.2015.12.047
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