Generation of KCL017 research grade human embryonic stem cell line carrying a mutation in VHL gene

The KCL017 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting splicing site of the VHL gene encoding von Hippel–Lindau tumor suppressor E3 ubiquitin protein ligase (676 + 3 A > T). The ICM was isolated using laser m...

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Autores principales: Hewitson, Heema, Wood, Victoria, Kadeva, Neli, Cornwell, Glenda, Codognotto, Stefano, Stephenson, Emma, Ilic, Dusko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Lab Resource: Stem Cell Line
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823763/
https://www.ncbi.nlm.nih.gov/pubmed/27345980
http://dx.doi.org/10.1016/j.scr.2016.01.013
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author Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_facet Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_sort Hewitson, Heema
collection PubMed
description The KCL017 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting splicing site of the VHL gene encoding von Hippel–Lindau tumor suppressor E3 ubiquitin protein ligase (676 + 3 A > T). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.
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spelling pubmed-48237632016-04-15 Generation of KCL017 research grade human embryonic stem cell line carrying a mutation in VHL gene Hewitson, Heema Wood, Victoria Kadeva, Neli Cornwell, Glenda Codognotto, Stefano Stephenson, Emma Ilic, Dusko Stem Cell Res Lab Resource: Stem Cell Line The KCL017 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting splicing site of the VHL gene encoding von Hippel–Lindau tumor suppressor E3 ubiquitin protein ligase (676 + 3 A > T). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays. Elsevier 2016-03 /pmc/articles/PMC4823763/ /pubmed/27345980 http://dx.doi.org/10.1016/j.scr.2016.01.013 Text en © 2016 University of Texas at Austin Dell Medical School http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Lab Resource: Stem Cell Line
Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
Generation of KCL017 research grade human embryonic stem cell line carrying a mutation in VHL gene
title Generation of KCL017 research grade human embryonic stem cell line carrying a mutation in VHL gene
title_full Generation of KCL017 research grade human embryonic stem cell line carrying a mutation in VHL gene
title_fullStr Generation of KCL017 research grade human embryonic stem cell line carrying a mutation in VHL gene
title_full_unstemmed Generation of KCL017 research grade human embryonic stem cell line carrying a mutation in VHL gene
title_short Generation of KCL017 research grade human embryonic stem cell line carrying a mutation in VHL gene
title_sort generation of kcl017 research grade human embryonic stem cell line carrying a mutation in vhl gene
topic Lab Resource: Stem Cell Line
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823763/
https://www.ncbi.nlm.nih.gov/pubmed/27345980
http://dx.doi.org/10.1016/j.scr.2016.01.013
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