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Draft Genome Sequences of Two Closely Related Aflatoxigenic Aspergillus Species Obtained from the Ivory Coast

Aspergillus ochraceoroseus and Aspergillus rambellii were isolated from soil detritus in Taï National Park, Ivory Coast, Africa. The Type strain for each species happens to be the only representative ever sampled. Both species secrete copious amounts of aflatoxin B(1) and sterigmatocystin, because e...

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Autores principales: Moore, Geromy G., Mack, Brian M., Beltz, Shannon B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823974/
https://www.ncbi.nlm.nih.gov/pubmed/26637470
http://dx.doi.org/10.1093/gbe/evv246
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author Moore, Geromy G.
Mack, Brian M.
Beltz, Shannon B.
author_facet Moore, Geromy G.
Mack, Brian M.
Beltz, Shannon B.
author_sort Moore, Geromy G.
collection PubMed
description Aspergillus ochraceoroseus and Aspergillus rambellii were isolated from soil detritus in Taï National Park, Ivory Coast, Africa. The Type strain for each species happens to be the only representative ever sampled. Both species secrete copious amounts of aflatoxin B(1) and sterigmatocystin, because each of their genomes contains clustered genes for biosynthesis of these mycotoxins. We sequenced their genomes using a personal genome machine and found them to be smaller in size (A. ochraceoroseus = 23.9 Mb and A. rambellii = 26.1 Mb), as well as in numbers of predicted genes (7,837 and 7,807, respectively), compared to other sequenced Aspergilli. Our findings also showed that the A. ochraceoroseus Type strain contains a single MAT1-1 gene, while the Type strain of A. rambellii contains a single MAT1-2 gene, indicating that these species are heterothallic (self-infertile). These draft genomes will be useful for understanding the genes and pathways necessary for the cosynthesis of these two toxic secondary metabolites as well as the evolution of these pathways in aflatoxigenic fungi.
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spelling pubmed-48239742016-04-08 Draft Genome Sequences of Two Closely Related Aflatoxigenic Aspergillus Species Obtained from the Ivory Coast Moore, Geromy G. Mack, Brian M. Beltz, Shannon B. Genome Biol Evol Genome Report Aspergillus ochraceoroseus and Aspergillus rambellii were isolated from soil detritus in Taï National Park, Ivory Coast, Africa. The Type strain for each species happens to be the only representative ever sampled. Both species secrete copious amounts of aflatoxin B(1) and sterigmatocystin, because each of their genomes contains clustered genes for biosynthesis of these mycotoxins. We sequenced their genomes using a personal genome machine and found them to be smaller in size (A. ochraceoroseus = 23.9 Mb and A. rambellii = 26.1 Mb), as well as in numbers of predicted genes (7,837 and 7,807, respectively), compared to other sequenced Aspergilli. Our findings also showed that the A. ochraceoroseus Type strain contains a single MAT1-1 gene, while the Type strain of A. rambellii contains a single MAT1-2 gene, indicating that these species are heterothallic (self-infertile). These draft genomes will be useful for understanding the genes and pathways necessary for the cosynthesis of these two toxic secondary metabolites as well as the evolution of these pathways in aflatoxigenic fungi. Oxford University Press 2015-12-03 /pmc/articles/PMC4823974/ /pubmed/26637470 http://dx.doi.org/10.1093/gbe/evv246 Text en Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution 2015. This work is written by a US Government employee and is in the public domain in the US.
spellingShingle Genome Report
Moore, Geromy G.
Mack, Brian M.
Beltz, Shannon B.
Draft Genome Sequences of Two Closely Related Aflatoxigenic Aspergillus Species Obtained from the Ivory Coast
title Draft Genome Sequences of Two Closely Related Aflatoxigenic Aspergillus Species Obtained from the Ivory Coast
title_full Draft Genome Sequences of Two Closely Related Aflatoxigenic Aspergillus Species Obtained from the Ivory Coast
title_fullStr Draft Genome Sequences of Two Closely Related Aflatoxigenic Aspergillus Species Obtained from the Ivory Coast
title_full_unstemmed Draft Genome Sequences of Two Closely Related Aflatoxigenic Aspergillus Species Obtained from the Ivory Coast
title_short Draft Genome Sequences of Two Closely Related Aflatoxigenic Aspergillus Species Obtained from the Ivory Coast
title_sort draft genome sequences of two closely related aflatoxigenic aspergillus species obtained from the ivory coast
topic Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823974/
https://www.ncbi.nlm.nih.gov/pubmed/26637470
http://dx.doi.org/10.1093/gbe/evv246
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