Cargando…

Integrated microfluidic approach for quantitative high-throughput measurements of transcription factor binding affinities

Protein binding to DNA is a fundamental process in gene regulation. Methodologies such as ChIP-Seq and mapping of DNase I hypersensitive sites provide global information on this regulation in vivo. In vitro methodologies provide valuable complementary information on protein–DNA specificities. Howeve...

Descripción completa

Detalles Bibliográficos
Autores principales:	Glick, Yair, Orenstein, Yaron, Chen, Dana, Avrahami, Dorit, Zor, Tsaffrir, Shamir, Ron, Gerber, Doron
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2016
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824076/ https://www.ncbi.nlm.nih.gov/pubmed/26635393 http://dx.doi.org/10.1093/nar/gkv1327

Ejemplares similares

SELMAP - SELEX affinity landscape MAPping of transcription factor binding sites using integrated microfluidics
por: Chen, Dana, et al.
Publicado: (2016)

A comparative analysis of transcription factor binding models learned from PBM, HT-SELEX and ChIP data
por: Orenstein, Yaron, et al.
Publicado: (2014)

A high-throughput integrated microfluidics method enables tyrosine autophosphorylation discovery
por: Nevenzal, Hadas, et al.
Publicado: (2019)

Modeling protein–DNA binding via high-throughput in vitro technologies
por: Orenstein, Yaron, et al.
Publicado: (2017)

High-throughput quantitative binding analysis of DNA aptamers using exonucleases
por: Canoura, Juan, et al.
Publicado: (2022)

Quantitative analysis of the binding affinity of poly(ADP-ribose) to specific binding proteins as a function of chain length
por: Fahrer, Jörg, et al.
Publicado: (2007)

Exclusive Temporal Stimulation of IL-10 Expression in LPS-Stimulated Mouse Macrophages by cAMP Inducers and Type I Interferons
por: Ernst, Orna, et al.
Publicado: (2019)

Nanoliter high throughput quantitative PCR
por: Morrison, Tom, et al.
Publicado: (2006)

RNA–protein binding kinetics in an automated microfluidic reactor
por: Ridgeway, William K., et al.
Publicado: (2009)

Drosophila TRF2 is a preferential core promoter regulator
por: Kedmi, Adi, et al.
Publicado: (2014)

Engineering a high-affinity methyl-CpG-binding protein
por: Jørgensen, Helle F., et al.
Publicado: (2006)

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
por: Whale, Alexandra S., et al.
Publicado: (2012)

Single molecule quantitation and sequencing of rare translocations using microfluidic nested digital PCR
por: Shuga, Joe, et al.
Publicado: (2013)

Affinity selection of DNA-binding protein complexes using mRNA display
por: Tateyama, Seiji, et al.
Publicado: (2006)

A robust assay to measure DNA topology-dependent protein binding affinity
por: Litwin, Tamara R., et al.
Publicado: (2015)

Tandem phage-display for the identification of non-overlapping binding pairs of recombinant affinity reagents
por: Gorman, Kevin T., et al.
Publicado: (2017)

Identifying high-affinity aptamer ligands with defined cross-reactivity using high-throughput guided systematic evolution of ligands by exponential enrichment
por: Levay, Agata, et al.
Publicado: (2015)

Efficient minimizer orders for large values of k using minimum decycling sets
por: Pellow, David, et al.
Publicado: (2023)

From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing
por: Meyer, Matthias, et al.
Publicado: (2008)

A high-throughput and quantitative method to assess the mutagenic potential of translesion DNA synthesis
por: Taggart, David J., et al.
Publicado: (2013)

A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity
por: Liang, Joe C., et al.
Publicado: (2012)

Towards computational prediction of microRNA function and activity
por: Ulitsky, Igor, et al.
Publicado: (2010)

CT-FOCS: a novel method for inferring cell type-specific enhancer–promoter maps
por: Hait, Tom Aharon, et al.
Publicado: (2022)

Identification of target-binding peptide motifs by high-throughput sequencing of phage-selected peptides
por: Rentero Rebollo, Inmaculada, et al.
Publicado: (2014)

High-throughput prediction of RNA, DNA and protein binding regions mediated by intrinsic disorder
por: Peng, Zhenling, et al.
Publicado: (2015)

Computational modeling of microfluidic data provides high-throughput affinity estimates for monoclonal antibodies
por: Budroni, Sonia, et al.
Publicado: (2021)

A deep boosting based approach for capturing the sequence binding preferences of RNA-binding proteins from high-throughput CLIP-seq data
por: Li, Shuya, et al.
Publicado: (2017)

Microfluidic droplet enrichment for targeted sequencing
por: Eastburn, Dennis J., et al.
Publicado: (2015)

Bind-n-Seq: high-throughput analysis of in vitro protein–DNA interactions using massively parallel sequencing
por: Zykovich, Artem, et al.
Publicado: (2009)

Design of shortest double-stranded DNA sequences covering all k-mers with applications to protein-binding microarrays and synthetic enhancers
por: Orenstein, Yaron, et al.
Publicado: (2013)

Design of shortest double-stranded DNA sequences covering all k-mers with applications to protein-binding microarrays and synthetic enhancers
por: Orenstein, Yaron, et al.
Publicado: (2015)

Genomic profiling of CpG methylation and allelic specificity using quantitative high-throughput mass spectrometry: critical evaluation and improvements
por: Coolen, Marcel W., et al.
Publicado: (2007)

Assessment of Algorithms for Inferring Positional Weight Matrix Motifs of Transcription Factor Binding Sites Using Protein Binding Microarray Data
por: Orenstein, Yaron, et al.
Publicado: (2012)

Parallel gene synthesis in a microfluidic device
por: Kong, David S., et al.
Publicado: (2007)

Quantitative modeling of gene expression using DNA shape features of binding sites
por: Peng, Pei-Chen, et al.
Publicado: (2016)

High-throughput alternative splicing quantification by primer extension and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
por: McCullough, Ron M., et al.
Publicado: (2005)

Assessment of the optimization of affinity and specificity at protein–DNA interfaces
por: Ashworth, Justin, et al.
Publicado: (2009)

Quantitative analysis of transcription factor binding and expression using calling cards reporter arrays
por: Liu, Jiayue, et al.
Publicado: (2020)

Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
por: Brio, Lev, et al.
Publicado: (2022)

Sensitive Melting Analysis after Real Time- Methylation Specific PCR (SMART-MSP): high-throughput and probe-free quantitative DNA methylation detection
por: Kristensen, Lasse S., et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_akqincqvjdv0b9i1nvuv1fsag7