Cargando…

Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA

The RNase P-mediated endonucleolytic cleavage plays a crucial role in the 3′ end processing and cellular accumulation of MALAT1, a nuclear-retained long noncoding RNA that promotes malignancy. The regulation of this cleavage event is largely undetermined. Here we characterize a broadly expressed nat...

Descripción completa

Detalles Bibliográficos
Autores principales: Zong, Xinying, Nakagawa, Shinichi, Freier, Susan M., Fei, Jingyi, Ha, Taekjip, Prasanth, Supriya G., Prasanth, Kannanganattu V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824109/
https://www.ncbi.nlm.nih.gov/pubmed/26826711
http://dx.doi.org/10.1093/nar/gkw047
_version_ 1782426042659504128
author Zong, Xinying
Nakagawa, Shinichi
Freier, Susan M.
Fei, Jingyi
Ha, Taekjip
Prasanth, Supriya G.
Prasanth, Kannanganattu V.
author_facet Zong, Xinying
Nakagawa, Shinichi
Freier, Susan M.
Fei, Jingyi
Ha, Taekjip
Prasanth, Supriya G.
Prasanth, Kannanganattu V.
author_sort Zong, Xinying
collection PubMed
description The RNase P-mediated endonucleolytic cleavage plays a crucial role in the 3′ end processing and cellular accumulation of MALAT1, a nuclear-retained long noncoding RNA that promotes malignancy. The regulation of this cleavage event is largely undetermined. Here we characterize a broadly expressed natural antisense transcript at the MALAT1 locus, designated as TALAM1, that positively regulates MALAT1 levels by promoting the 3′ end cleavage and maturation of MALAT1 RNA. TALAM1 RNA preferentially localizes at the site of transcription, and also interacts with MALAT1 RNA. Depletion of TALAM1 leads to defects in the 3′ end cleavage reaction and compromises cellular accumulation of MALAT1. Conversely, overexpression of TALAM1 facilitates the cleavage reaction in trans. Interestingly, TALAM1 is also positively regulated by MALAT1 at the level of both transcription and RNA stability. Together, our data demonstrate a novel feed-forward positive regulatory loop that is established to maintain the high cellular levels of MALAT1, and also unravel the existence of sense-antisense mediated regulatory mechanism for cellular lncRNAs that display RNase P-mediated 3′ end processing.
format Online
Article
Text
id pubmed-4824109
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-48241092016-04-08 Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA Zong, Xinying Nakagawa, Shinichi Freier, Susan M. Fei, Jingyi Ha, Taekjip Prasanth, Supriya G. Prasanth, Kannanganattu V. Nucleic Acids Res RNA The RNase P-mediated endonucleolytic cleavage plays a crucial role in the 3′ end processing and cellular accumulation of MALAT1, a nuclear-retained long noncoding RNA that promotes malignancy. The regulation of this cleavage event is largely undetermined. Here we characterize a broadly expressed natural antisense transcript at the MALAT1 locus, designated as TALAM1, that positively regulates MALAT1 levels by promoting the 3′ end cleavage and maturation of MALAT1 RNA. TALAM1 RNA preferentially localizes at the site of transcription, and also interacts with MALAT1 RNA. Depletion of TALAM1 leads to defects in the 3′ end cleavage reaction and compromises cellular accumulation of MALAT1. Conversely, overexpression of TALAM1 facilitates the cleavage reaction in trans. Interestingly, TALAM1 is also positively regulated by MALAT1 at the level of both transcription and RNA stability. Together, our data demonstrate a novel feed-forward positive regulatory loop that is established to maintain the high cellular levels of MALAT1, and also unravel the existence of sense-antisense mediated regulatory mechanism for cellular lncRNAs that display RNase P-mediated 3′ end processing. Oxford University Press 2016-04-07 2016-01-29 /pmc/articles/PMC4824109/ /pubmed/26826711 http://dx.doi.org/10.1093/nar/gkw047 Text en © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle RNA
Zong, Xinying
Nakagawa, Shinichi
Freier, Susan M.
Fei, Jingyi
Ha, Taekjip
Prasanth, Supriya G.
Prasanth, Kannanganattu V.
Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA
title Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA
title_full Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA
title_fullStr Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA
title_full_unstemmed Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA
title_short Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA
title_sort natural antisense rna promotes 3′ end processing and maturation of malat1 lncrna
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824109/
https://www.ncbi.nlm.nih.gov/pubmed/26826711
http://dx.doi.org/10.1093/nar/gkw047
work_keys_str_mv AT zongxinying naturalantisensernapromotes3endprocessingandmaturationofmalat1lncrna
AT nakagawashinichi naturalantisensernapromotes3endprocessingandmaturationofmalat1lncrna
AT freiersusanm naturalantisensernapromotes3endprocessingandmaturationofmalat1lncrna
AT feijingyi naturalantisensernapromotes3endprocessingandmaturationofmalat1lncrna
AT hataekjip naturalantisensernapromotes3endprocessingandmaturationofmalat1lncrna
AT prasanthsupriyag naturalantisensernapromotes3endprocessingandmaturationofmalat1lncrna
AT prasanthkannanganattuv naturalantisensernapromotes3endprocessingandmaturationofmalat1lncrna