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Phosphatidylserine Synthase Controls Cell Elongation Especially in the Uppermost Internode in Rice by Regulation of Exocytosis

The uppermost internode is one of the fastest elongating organs in rice, and is expected to require an adequate supply of cell-wall materials and enzymes to the cell surface to enhance mechanical strength. Although it has been reported that the phenotype of shortened uppermost internode 1 (sui1) is...

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Autores principales: Ma, Jin, Cheng, Zhijun, Chen, Jun, Shen, Jinbo, Zhang, Baocai, Ren, Yulong, Ding, Yu, Zhou, Yihua, Zhang, Huan, Zhou, Kunneng, Wang, Jiu-Lin, Lei, Cailin, Zhang, Xin, Guo, Xiuping, Gao, He, Bao, Yiqun, Wan, Jian-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824389/
https://www.ncbi.nlm.nih.gov/pubmed/27055010
http://dx.doi.org/10.1371/journal.pone.0153119
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author Ma, Jin
Cheng, Zhijun
Chen, Jun
Shen, Jinbo
Zhang, Baocai
Ren, Yulong
Ding, Yu
Zhou, Yihua
Zhang, Huan
Zhou, Kunneng
Wang, Jiu-Lin
Lei, Cailin
Zhang, Xin
Guo, Xiuping
Gao, He
Bao, Yiqun
Wan, Jian-Min
author_facet Ma, Jin
Cheng, Zhijun
Chen, Jun
Shen, Jinbo
Zhang, Baocai
Ren, Yulong
Ding, Yu
Zhou, Yihua
Zhang, Huan
Zhou, Kunneng
Wang, Jiu-Lin
Lei, Cailin
Zhang, Xin
Guo, Xiuping
Gao, He
Bao, Yiqun
Wan, Jian-Min
author_sort Ma, Jin
collection PubMed
description The uppermost internode is one of the fastest elongating organs in rice, and is expected to require an adequate supply of cell-wall materials and enzymes to the cell surface to enhance mechanical strength. Although it has been reported that the phenotype of shortened uppermost internode 1 (sui1) is caused by mutations in PHOSPHATIDYLSERINE SYNTHASE (OsPSS), the underlying mechanism remains unclear. Here we show that the OsPSS-1, as a gene expressed predominantly in elongating cells, regulates post-Golgi vesicle secretion to intercellular spaces. Mutation of OsPSS-1 leads to compromised delivery of CESA4 and secGFP towards the cell surface, resulting in weakened intercellular adhesion and disorganized cell arrangement in parenchyma. The phenotype of sui1-4 is caused largely by the reduction in cellulose contents in the whole plant and detrimental delivery of pectins in the uppermost internode. We found that OsPSS-1 and its potential product PS (phosphatidylserine) localized to organelles associated with exocytosis. These results together suggest that OsPSS-1 plays a potential role in mediating cell expansion by regulating secretion of cell wall components.
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spelling pubmed-48243892016-04-22 Phosphatidylserine Synthase Controls Cell Elongation Especially in the Uppermost Internode in Rice by Regulation of Exocytosis Ma, Jin Cheng, Zhijun Chen, Jun Shen, Jinbo Zhang, Baocai Ren, Yulong Ding, Yu Zhou, Yihua Zhang, Huan Zhou, Kunneng Wang, Jiu-Lin Lei, Cailin Zhang, Xin Guo, Xiuping Gao, He Bao, Yiqun Wan, Jian-Min PLoS One Research Article The uppermost internode is one of the fastest elongating organs in rice, and is expected to require an adequate supply of cell-wall materials and enzymes to the cell surface to enhance mechanical strength. Although it has been reported that the phenotype of shortened uppermost internode 1 (sui1) is caused by mutations in PHOSPHATIDYLSERINE SYNTHASE (OsPSS), the underlying mechanism remains unclear. Here we show that the OsPSS-1, as a gene expressed predominantly in elongating cells, regulates post-Golgi vesicle secretion to intercellular spaces. Mutation of OsPSS-1 leads to compromised delivery of CESA4 and secGFP towards the cell surface, resulting in weakened intercellular adhesion and disorganized cell arrangement in parenchyma. The phenotype of sui1-4 is caused largely by the reduction in cellulose contents in the whole plant and detrimental delivery of pectins in the uppermost internode. We found that OsPSS-1 and its potential product PS (phosphatidylserine) localized to organelles associated with exocytosis. These results together suggest that OsPSS-1 plays a potential role in mediating cell expansion by regulating secretion of cell wall components. Public Library of Science 2016-04-07 /pmc/articles/PMC4824389/ /pubmed/27055010 http://dx.doi.org/10.1371/journal.pone.0153119 Text en © 2016 Ma et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ma, Jin
Cheng, Zhijun
Chen, Jun
Shen, Jinbo
Zhang, Baocai
Ren, Yulong
Ding, Yu
Zhou, Yihua
Zhang, Huan
Zhou, Kunneng
Wang, Jiu-Lin
Lei, Cailin
Zhang, Xin
Guo, Xiuping
Gao, He
Bao, Yiqun
Wan, Jian-Min
Phosphatidylserine Synthase Controls Cell Elongation Especially in the Uppermost Internode in Rice by Regulation of Exocytosis
title Phosphatidylserine Synthase Controls Cell Elongation Especially in the Uppermost Internode in Rice by Regulation of Exocytosis
title_full Phosphatidylserine Synthase Controls Cell Elongation Especially in the Uppermost Internode in Rice by Regulation of Exocytosis
title_fullStr Phosphatidylserine Synthase Controls Cell Elongation Especially in the Uppermost Internode in Rice by Regulation of Exocytosis
title_full_unstemmed Phosphatidylserine Synthase Controls Cell Elongation Especially in the Uppermost Internode in Rice by Regulation of Exocytosis
title_short Phosphatidylserine Synthase Controls Cell Elongation Especially in the Uppermost Internode in Rice by Regulation of Exocytosis
title_sort phosphatidylserine synthase controls cell elongation especially in the uppermost internode in rice by regulation of exocytosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824389/
https://www.ncbi.nlm.nih.gov/pubmed/27055010
http://dx.doi.org/10.1371/journal.pone.0153119
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