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Rapid and Inexpensive Method of Loading Fluorescent Dye into Pollen Tubes and Root Hairs
The most direct technique for studying calcium, which is an essential element for pollen tube growth, is Ca(2+) imaging. Because membranes are relatively impermeable, the loading of fluorescent Ca(2+) probes into plant cells is a challenging task. Thus, we have developed a new method of loading fluo...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824429/ https://www.ncbi.nlm.nih.gov/pubmed/27055240 http://dx.doi.org/10.1371/journal.pone.0152320 |