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Determination of Intracellular Vitrification Temperatures for Unicellular Micro Organisms under Conditions Relevant for Cryopreservation

During cryopreservation ice nucleation and crystal growth may occur within cells or the intracellular compartment may vitrify. Whilst previous literature describes intracellular vitrification in a qualitative manner, here we measure the intracellular vitrification temperature of bacteria and yeasts...

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Autores principales: Fonseca, Fernanda, Meneghel, Julie, Cenard, Stéphanie, Passot, Stéphanie, Morris, G. John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824440/
https://www.ncbi.nlm.nih.gov/pubmed/27055246
http://dx.doi.org/10.1371/journal.pone.0152939
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author Fonseca, Fernanda
Meneghel, Julie
Cenard, Stéphanie
Passot, Stéphanie
Morris, G. John
author_facet Fonseca, Fernanda
Meneghel, Julie
Cenard, Stéphanie
Passot, Stéphanie
Morris, G. John
author_sort Fonseca, Fernanda
collection PubMed
description During cryopreservation ice nucleation and crystal growth may occur within cells or the intracellular compartment may vitrify. Whilst previous literature describes intracellular vitrification in a qualitative manner, here we measure the intracellular vitrification temperature of bacteria and yeasts under conditions relevant to cryopreservation, including the addition of high levels of permeating and nonpermeating additives and the application of rapid rates of cooling. The effects of growth conditions that are known to modify cellular freezing resistance on the intracellular vitrification temperature are also examined. For bacteria a plot of the activity on thawing against intracellular glass transition of the maximally freeze-concentrated matrix (Tg’) shows that cells with the lowest value of intracellular Tg’ survive the freezing process better than cells with a higher intracellular Tg’. This paper demonstrates the role of the physical state of the intracellular environment in determining the response of microbial cells to preservation and could be a powerful tool to be manipulated to allow the optimization of methods for the preservation of microorganisms.
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spelling pubmed-48244402016-04-22 Determination of Intracellular Vitrification Temperatures for Unicellular Micro Organisms under Conditions Relevant for Cryopreservation Fonseca, Fernanda Meneghel, Julie Cenard, Stéphanie Passot, Stéphanie Morris, G. John PLoS One Research Article During cryopreservation ice nucleation and crystal growth may occur within cells or the intracellular compartment may vitrify. Whilst previous literature describes intracellular vitrification in a qualitative manner, here we measure the intracellular vitrification temperature of bacteria and yeasts under conditions relevant to cryopreservation, including the addition of high levels of permeating and nonpermeating additives and the application of rapid rates of cooling. The effects of growth conditions that are known to modify cellular freezing resistance on the intracellular vitrification temperature are also examined. For bacteria a plot of the activity on thawing against intracellular glass transition of the maximally freeze-concentrated matrix (Tg’) shows that cells with the lowest value of intracellular Tg’ survive the freezing process better than cells with a higher intracellular Tg’. This paper demonstrates the role of the physical state of the intracellular environment in determining the response of microbial cells to preservation and could be a powerful tool to be manipulated to allow the optimization of methods for the preservation of microorganisms. Public Library of Science 2016-04-07 /pmc/articles/PMC4824440/ /pubmed/27055246 http://dx.doi.org/10.1371/journal.pone.0152939 Text en © 2016 Fonseca et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Fonseca, Fernanda
Meneghel, Julie
Cenard, Stéphanie
Passot, Stéphanie
Morris, G. John
Determination of Intracellular Vitrification Temperatures for Unicellular Micro Organisms under Conditions Relevant for Cryopreservation
title Determination of Intracellular Vitrification Temperatures for Unicellular Micro Organisms under Conditions Relevant for Cryopreservation
title_full Determination of Intracellular Vitrification Temperatures for Unicellular Micro Organisms under Conditions Relevant for Cryopreservation
title_fullStr Determination of Intracellular Vitrification Temperatures for Unicellular Micro Organisms under Conditions Relevant for Cryopreservation
title_full_unstemmed Determination of Intracellular Vitrification Temperatures for Unicellular Micro Organisms under Conditions Relevant for Cryopreservation
title_short Determination of Intracellular Vitrification Temperatures for Unicellular Micro Organisms under Conditions Relevant for Cryopreservation
title_sort determination of intracellular vitrification temperatures for unicellular micro organisms under conditions relevant for cryopreservation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824440/
https://www.ncbi.nlm.nih.gov/pubmed/27055246
http://dx.doi.org/10.1371/journal.pone.0152939
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