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Evaluation of subacute bisphenol – A toxicity on male reproductive system

AIM: The aim was to evaluate the effect of multiple oral administration of bisphenol A (BPA) for 28 days on seminal characteristic on mammal using Wistar rat as a model. MATERIALS AND METHODS: Rats were randomly divided into five different groups having 6 male rats in each group. The doses chosen we...

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Detalles Bibliográficos
Autores principales: Karnam, S. S., Ghosh, R. C., Mondal, S., Mondal, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4825275/
https://www.ncbi.nlm.nih.gov/pubmed/27065640
http://dx.doi.org/10.14202/vetworld.2015.738-744
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author Karnam, S. S.
Ghosh, R. C.
Mondal, S.
Mondal, M.
author_facet Karnam, S. S.
Ghosh, R. C.
Mondal, S.
Mondal, M.
author_sort Karnam, S. S.
collection PubMed
description AIM: The aim was to evaluate the effect of multiple oral administration of bisphenol A (BPA) for 28 days on seminal characteristic on mammal using Wistar rat as a model. MATERIALS AND METHODS: Rats were randomly divided into five different groups having 6 male rats in each group. The doses chosen were 50, 200, and 600 mg/kg body weight for Groups III, IV and V, respectively, based on preliminary dose range finding study and Group II served as vehicle control and Group I was negative control. RESULTS: Reproductive study in the BPA-treated rats on day 28 revealed that there was significant (p≤0.05) reduction in the epididymal sperm count of rats of Group IV and significant (p≤0.01) decrease in Group V. Sperm motility percentage, dead count percentage, head and tail abnormality percentage were found to be significantly (p≤0.01) increased in rats of BPA-treated groups as compared to rats of control groups. Testes showed necrosis of germinal layer and spermatogonial cells in the seminiferous tubules. Hematological examination revealed significant (p≤0.01) decrease in the mean values of total erythrocyte count (TEC), total leukocyte count (TLC), hemoglobin, packed cell volume, and there was also significant (p≤0.05) lymphocytopenia in treated animals. CONCLUSION: It can be concluded from this study that subacute toxicity of BPA caused a reduction in the epididymal sperm count, sperm motility, dead count, head and tail abnormality, as well as hematological indices such as TLC, TEC etc. Hence, it appears that BPA affects the germ cells leading to impairment in the spermatogenesis, and thus having its property as reproductive toxicant and it also suppresses bone marrow functioning, which leads to normocytic hypochromic anemia in rats.
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spelling pubmed-48252752016-04-08 Evaluation of subacute bisphenol – A toxicity on male reproductive system Karnam, S. S. Ghosh, R. C. Mondal, S. Mondal, M. Vet World Research Article AIM: The aim was to evaluate the effect of multiple oral administration of bisphenol A (BPA) for 28 days on seminal characteristic on mammal using Wistar rat as a model. MATERIALS AND METHODS: Rats were randomly divided into five different groups having 6 male rats in each group. The doses chosen were 50, 200, and 600 mg/kg body weight for Groups III, IV and V, respectively, based on preliminary dose range finding study and Group II served as vehicle control and Group I was negative control. RESULTS: Reproductive study in the BPA-treated rats on day 28 revealed that there was significant (p≤0.05) reduction in the epididymal sperm count of rats of Group IV and significant (p≤0.01) decrease in Group V. Sperm motility percentage, dead count percentage, head and tail abnormality percentage were found to be significantly (p≤0.01) increased in rats of BPA-treated groups as compared to rats of control groups. Testes showed necrosis of germinal layer and spermatogonial cells in the seminiferous tubules. Hematological examination revealed significant (p≤0.01) decrease in the mean values of total erythrocyte count (TEC), total leukocyte count (TLC), hemoglobin, packed cell volume, and there was also significant (p≤0.05) lymphocytopenia in treated animals. CONCLUSION: It can be concluded from this study that subacute toxicity of BPA caused a reduction in the epididymal sperm count, sperm motility, dead count, head and tail abnormality, as well as hematological indices such as TLC, TEC etc. Hence, it appears that BPA affects the germ cells leading to impairment in the spermatogenesis, and thus having its property as reproductive toxicant and it also suppresses bone marrow functioning, which leads to normocytic hypochromic anemia in rats. Veterinary World 2015-06 2015-06-17 /pmc/articles/PMC4825275/ /pubmed/27065640 http://dx.doi.org/10.14202/vetworld.2015.738-744 Text en Copyright: © The authors. http://creativecommons.org/licenses/by/2.0 This article is an open access article licensed under the terms of the Creative Commons Attributin License (http://creative commons.org/licenses/by/2.0) which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Research Article
Karnam, S. S.
Ghosh, R. C.
Mondal, S.
Mondal, M.
Evaluation of subacute bisphenol – A toxicity on male reproductive system
title Evaluation of subacute bisphenol – A toxicity on male reproductive system
title_full Evaluation of subacute bisphenol – A toxicity on male reproductive system
title_fullStr Evaluation of subacute bisphenol – A toxicity on male reproductive system
title_full_unstemmed Evaluation of subacute bisphenol – A toxicity on male reproductive system
title_short Evaluation of subacute bisphenol – A toxicity on male reproductive system
title_sort evaluation of subacute bisphenol – a toxicity on male reproductive system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4825275/
https://www.ncbi.nlm.nih.gov/pubmed/27065640
http://dx.doi.org/10.14202/vetworld.2015.738-744
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