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Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections
BACKGROUND: Pasteurella multocida a Gram-negative bacterium has been identified as the causative agent of many economically important diseases in a wide range of hosts. Hemorrhagic septicemia is a disease caused by P. multocida serotype B:2 and E:2. The organism causes acute, a highly fatal septicem...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4825283/ https://www.ncbi.nlm.nih.gov/pubmed/27065648 http://dx.doi.org/10.14202/vetworld.2015.783-792 |
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author | Chung, Eric Lim Teik Abdullah, Faez Firdaus Jesse Adamu, Lawan Marza, Ali Dhiaa Ibrahim, Hayder Hamzah Zamri-Saad, Mohd Haron, Abdul Wahid Saharee, Abdul Aziz Lila, Mohd Azmi Mohd Omar, Abdul Rahman Bakar, Md Zuki Abu Norsidin, Mohd Jefri |
author_facet | Chung, Eric Lim Teik Abdullah, Faez Firdaus Jesse Adamu, Lawan Marza, Ali Dhiaa Ibrahim, Hayder Hamzah Zamri-Saad, Mohd Haron, Abdul Wahid Saharee, Abdul Aziz Lila, Mohd Azmi Mohd Omar, Abdul Rahman Bakar, Md Zuki Abu Norsidin, Mohd Jefri |
author_sort | Chung, Eric Lim Teik |
collection | PubMed |
description | BACKGROUND: Pasteurella multocida a Gram-negative bacterium has been identified as the causative agent of many economically important diseases in a wide range of hosts. Hemorrhagic septicemia is a disease caused by P. multocida serotype B:2 and E:2. The organism causes acute, a highly fatal septicemic disease with high morbidity and mortality in cattle and more susceptible in buffaloes. Therefore, the aim of this study was to investigate the clinical signs, blood parameters, post mortem and histopathology changes caused by P. multocida Type B:2 infections initiated through the oral and subcutaneous routes. METHODS: Nine buffalo heifers were divided equally into 3 treatment groups. Group 1 was inoculated orally with 10 ml of phosphate buffer saline; Groups 2 and 3 were inoculated with 10 ml of 10(12) colony forming unit of P. multocida Type B:2 subcutaneously and orally respectively. RESULTS: There was a significant difference (p<0.05) in temperature between the subcutaneous and the control group. The results revealed significant differences (p<0.05) in erythrocytes, hemoglobin, packed cell volume, leukocytes, monocytes, and A: G ratio between the subcutaneous and the control group. Furthermore, there were significant differences (p<0.05) in leukocytes, band neutrophils, segmented neutrophils, lymphocytes, eosinophils, basophils, thrombocytes, plasma protein, icterus index, gamma glutamyl tranferase and A: G ratio between the oral and the control group. The post mortem lesions of the subcutaneous group buffaloes showed generalized hyperemia, congestion and hemorrhage of the immune organs, gastro-intestinal tract organs and vital organs. The oral group buffaloes showed mild lesions in the lung and liver. Histologically, there were significant differences (p<0.05) in hemorrhage and congestion; necrosis and degeneration; inflammatory cells infiltration; and edema in between the groups. CONCLUSION: This study was a proof that oral route infection of P. multocida Type B:2 can be used to stimulate host cell responses where oral vaccine through feed can be developed in the near future. |
format | Online Article Text |
id | pubmed-4825283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-48252832016-04-08 Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections Chung, Eric Lim Teik Abdullah, Faez Firdaus Jesse Adamu, Lawan Marza, Ali Dhiaa Ibrahim, Hayder Hamzah Zamri-Saad, Mohd Haron, Abdul Wahid Saharee, Abdul Aziz Lila, Mohd Azmi Mohd Omar, Abdul Rahman Bakar, Md Zuki Abu Norsidin, Mohd Jefri Vet World Research Article BACKGROUND: Pasteurella multocida a Gram-negative bacterium has been identified as the causative agent of many economically important diseases in a wide range of hosts. Hemorrhagic septicemia is a disease caused by P. multocida serotype B:2 and E:2. The organism causes acute, a highly fatal septicemic disease with high morbidity and mortality in cattle and more susceptible in buffaloes. Therefore, the aim of this study was to investigate the clinical signs, blood parameters, post mortem and histopathology changes caused by P. multocida Type B:2 infections initiated through the oral and subcutaneous routes. METHODS: Nine buffalo heifers were divided equally into 3 treatment groups. Group 1 was inoculated orally with 10 ml of phosphate buffer saline; Groups 2 and 3 were inoculated with 10 ml of 10(12) colony forming unit of P. multocida Type B:2 subcutaneously and orally respectively. RESULTS: There was a significant difference (p<0.05) in temperature between the subcutaneous and the control group. The results revealed significant differences (p<0.05) in erythrocytes, hemoglobin, packed cell volume, leukocytes, monocytes, and A: G ratio between the subcutaneous and the control group. Furthermore, there were significant differences (p<0.05) in leukocytes, band neutrophils, segmented neutrophils, lymphocytes, eosinophils, basophils, thrombocytes, plasma protein, icterus index, gamma glutamyl tranferase and A: G ratio between the oral and the control group. The post mortem lesions of the subcutaneous group buffaloes showed generalized hyperemia, congestion and hemorrhage of the immune organs, gastro-intestinal tract organs and vital organs. The oral group buffaloes showed mild lesions in the lung and liver. Histologically, there were significant differences (p<0.05) in hemorrhage and congestion; necrosis and degeneration; inflammatory cells infiltration; and edema in between the groups. CONCLUSION: This study was a proof that oral route infection of P. multocida Type B:2 can be used to stimulate host cell responses where oral vaccine through feed can be developed in the near future. Veterinary World 2015-06 2015-06-24 /pmc/articles/PMC4825283/ /pubmed/27065648 http://dx.doi.org/10.14202/vetworld.2015.783-792 Text en Copyright: © The authors. http://creativecommons.org/licenses/by/2.0 This article is an open access article licensed under the terms of the Creative Commons Attributin License (http://creative commons.org/licenses/by/2.0) which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited. |
spellingShingle | Research Article Chung, Eric Lim Teik Abdullah, Faez Firdaus Jesse Adamu, Lawan Marza, Ali Dhiaa Ibrahim, Hayder Hamzah Zamri-Saad, Mohd Haron, Abdul Wahid Saharee, Abdul Aziz Lila, Mohd Azmi Mohd Omar, Abdul Rahman Bakar, Md Zuki Abu Norsidin, Mohd Jefri Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections |
title | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections |
title_full | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections |
title_fullStr | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections |
title_full_unstemmed | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections |
title_short | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections |
title_sort | clinico-pathology, hematology, and biochemistry responses toward pasteurella multocida type b: 2 via oral and subcutaneous route of infections |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4825283/ https://www.ncbi.nlm.nih.gov/pubmed/27065648 http://dx.doi.org/10.14202/vetworld.2015.783-792 |
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