Cargando…

Microarray Analysis of Gene Expression in Saccharomyces cerevisiae kap108Δ Mutants upon Addition of Oxidative Stress

Protein transport between the nucleus and cytoplasm of eukaryotic cells is tightly regulated, providing a mechanism for controlling intracellular localization of proteins, and regulating gene expression. In this study, we have investigated the importance of nucleocytoplasmic transport mediated by th...

Descripción completa

Detalles Bibliográficos
Autores principales: Belanger, Kenneth D., Larson, Nathaniel, Kahn, Jonathan, Tkachev, Dmitry, Ay, Ahmet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4825647/
https://www.ncbi.nlm.nih.gov/pubmed/26888869
http://dx.doi.org/10.1534/g3.116.027011
_version_ 1782426249439739904
author Belanger, Kenneth D.
Larson, Nathaniel
Kahn, Jonathan
Tkachev, Dmitry
Ay, Ahmet
author_facet Belanger, Kenneth D.
Larson, Nathaniel
Kahn, Jonathan
Tkachev, Dmitry
Ay, Ahmet
author_sort Belanger, Kenneth D.
collection PubMed
description Protein transport between the nucleus and cytoplasm of eukaryotic cells is tightly regulated, providing a mechanism for controlling intracellular localization of proteins, and regulating gene expression. In this study, we have investigated the importance of nucleocytoplasmic transport mediated by the karyopherin Kap108 in regulating cellular responses to oxidative stress in Saccharomyces cerevisiae. We carried out microarray analyses on wild-type and kap108 mutant cells grown under normal conditions, shortly after introduction of oxidative stress, after 1 hr of oxidative stress, and 1 hr after oxidative stress was removed. We observe more than 500 genes that undergo a 40% or greater change in differential expression between wild-type and kap108Δ cells under at least one of these conditions. Genes undergoing changes in expression can be categorized in two general groups: 1) those that are differentially expressed between wild-type and kap108Δ cells, no matter the oxidative stress conditions; and 2) those that have patterns of response dependent upon both the absence of Kap108, and introduction or removal of oxidative stress. Gene ontology analysis reveals that, among the genes whose expression is reduced in the absence of Kap108 are those involved in stress response and intracellular transport, while those overexpressed are largely involved in mating and pheromone response. We also identified 25 clusters of genes that undergo similar patterns of change in gene expression when oxidative stresses are added and subsequently removed, including genes involved in stress response, oxidation–reduction processing, iron homeostasis, ascospore wall assembly, transmembrane transport, and cell fusion during mating. These data suggest that Kap108 is important for regulating expression of genes involved in a variety of specific cell functions.
format Online
Article
Text
id pubmed-4825647
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Genetics Society of America
record_format MEDLINE/PubMed
spelling pubmed-48256472016-04-11 Microarray Analysis of Gene Expression in Saccharomyces cerevisiae kap108Δ Mutants upon Addition of Oxidative Stress Belanger, Kenneth D. Larson, Nathaniel Kahn, Jonathan Tkachev, Dmitry Ay, Ahmet G3 (Bethesda) Mutant Screen Report Protein transport between the nucleus and cytoplasm of eukaryotic cells is tightly regulated, providing a mechanism for controlling intracellular localization of proteins, and regulating gene expression. In this study, we have investigated the importance of nucleocytoplasmic transport mediated by the karyopherin Kap108 in regulating cellular responses to oxidative stress in Saccharomyces cerevisiae. We carried out microarray analyses on wild-type and kap108 mutant cells grown under normal conditions, shortly after introduction of oxidative stress, after 1 hr of oxidative stress, and 1 hr after oxidative stress was removed. We observe more than 500 genes that undergo a 40% or greater change in differential expression between wild-type and kap108Δ cells under at least one of these conditions. Genes undergoing changes in expression can be categorized in two general groups: 1) those that are differentially expressed between wild-type and kap108Δ cells, no matter the oxidative stress conditions; and 2) those that have patterns of response dependent upon both the absence of Kap108, and introduction or removal of oxidative stress. Gene ontology analysis reveals that, among the genes whose expression is reduced in the absence of Kap108 are those involved in stress response and intracellular transport, while those overexpressed are largely involved in mating and pheromone response. We also identified 25 clusters of genes that undergo similar patterns of change in gene expression when oxidative stresses are added and subsequently removed, including genes involved in stress response, oxidation–reduction processing, iron homeostasis, ascospore wall assembly, transmembrane transport, and cell fusion during mating. These data suggest that Kap108 is important for regulating expression of genes involved in a variety of specific cell functions. Genetics Society of America 2016-02-17 /pmc/articles/PMC4825647/ /pubmed/26888869 http://dx.doi.org/10.1534/g3.116.027011 Text en Copyright © 2016 Belanger et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Mutant Screen Report
Belanger, Kenneth D.
Larson, Nathaniel
Kahn, Jonathan
Tkachev, Dmitry
Ay, Ahmet
Microarray Analysis of Gene Expression in Saccharomyces cerevisiae kap108Δ Mutants upon Addition of Oxidative Stress
title Microarray Analysis of Gene Expression in Saccharomyces cerevisiae kap108Δ Mutants upon Addition of Oxidative Stress
title_full Microarray Analysis of Gene Expression in Saccharomyces cerevisiae kap108Δ Mutants upon Addition of Oxidative Stress
title_fullStr Microarray Analysis of Gene Expression in Saccharomyces cerevisiae kap108Δ Mutants upon Addition of Oxidative Stress
title_full_unstemmed Microarray Analysis of Gene Expression in Saccharomyces cerevisiae kap108Δ Mutants upon Addition of Oxidative Stress
title_short Microarray Analysis of Gene Expression in Saccharomyces cerevisiae kap108Δ Mutants upon Addition of Oxidative Stress
title_sort microarray analysis of gene expression in saccharomyces cerevisiae kap108δ mutants upon addition of oxidative stress
topic Mutant Screen Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4825647/
https://www.ncbi.nlm.nih.gov/pubmed/26888869
http://dx.doi.org/10.1534/g3.116.027011
work_keys_str_mv AT belangerkennethd microarrayanalysisofgeneexpressioninsaccharomycescerevisiaekap108dmutantsuponadditionofoxidativestress
AT larsonnathaniel microarrayanalysisofgeneexpressioninsaccharomycescerevisiaekap108dmutantsuponadditionofoxidativestress
AT kahnjonathan microarrayanalysisofgeneexpressioninsaccharomycescerevisiaekap108dmutantsuponadditionofoxidativestress
AT tkachevdmitry microarrayanalysisofgeneexpressioninsaccharomycescerevisiaekap108dmutantsuponadditionofoxidativestress
AT ayahmet microarrayanalysisofgeneexpressioninsaccharomycescerevisiaekap108dmutantsuponadditionofoxidativestress