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Identification and Characterization of CD133(pos) Subpopulation Cells From a Human Laryngeal Cancer Cell Line
BACKGROUND: Recent research indicates that CD133 are expressed in several kinds of stem cells, among which, its high expression in laryngeal carcinoma has caused wide concern. To further explore efficaciously targeting drugs to laryngeal carcinoma stem cells (CSCs), we transplanted a solid tumor fro...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4825878/ https://www.ncbi.nlm.nih.gov/pubmed/27049928 http://dx.doi.org/10.12659/MSM.895645 |
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author | Qiu, Hai-ou Wang, Huifang Che, Na Li, Dong Mao, Yong Zeng, Qiao Ge, Rongming |
author_facet | Qiu, Hai-ou Wang, Huifang Che, Na Li, Dong Mao, Yong Zeng, Qiao Ge, Rongming |
author_sort | Qiu, Hai-ou |
collection | PubMed |
description | BACKGROUND: Recent research indicates that CD133 are expressed in several kinds of stem cells, among which, its high expression in laryngeal carcinoma has caused wide concern. To further explore efficaciously targeting drugs to laryngeal carcinoma stem cells (CSCs), we transplanted a solid tumor from CSCs into abdominal subcutaneous tissue of nude mice, and then compared the biological characteristics of laryngeal solid tumors with or without cisplatin intervention. MATERIAL/METHODS: In this study, the expression of CD133 was detected in the Hep-2 cell line by flow cytometry. By applying magnetic cell sorting (MACS) technology, we reported the results of purifying CD133-positive cells from a Hep-2 cell line. Cell proliferation, colony formation, and tumor-forming ability were examined in vitro and in vivo to identify the marker of CSCs in Hep-2 cell line. RESULTS: Upon flow cytometry analysis, CD133 was expressed constantly on 40.12±1.32% in Hep-2 cell line. Cell proliferation and colony formation ability were higher in CD133-positive cells compared to CD133-negative cells, and the in vivo tumorigenesis experiment showed the same results as in vitro assay. The 2 subpopulations cells were both sensitive to DDP, among which, the effect of DPP on proliferation ability and tumor-forming ability of CD133-positive cells was obviously greater than that of CD133-negative cells. CONCLUSIONS: Above all, our study revealed that CD133-positive cells have properties of higher proliferation, colony formation, and tumorigenesis in Hep-2 cell line, indicating that CD133 could be a marker to characterize laryngeal cancer stem cells. |
format | Online Article Text |
id | pubmed-4825878 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-48258782016-04-21 Identification and Characterization of CD133(pos) Subpopulation Cells From a Human Laryngeal Cancer Cell Line Qiu, Hai-ou Wang, Huifang Che, Na Li, Dong Mao, Yong Zeng, Qiao Ge, Rongming Med Sci Monit Molecular Biology BACKGROUND: Recent research indicates that CD133 are expressed in several kinds of stem cells, among which, its high expression in laryngeal carcinoma has caused wide concern. To further explore efficaciously targeting drugs to laryngeal carcinoma stem cells (CSCs), we transplanted a solid tumor from CSCs into abdominal subcutaneous tissue of nude mice, and then compared the biological characteristics of laryngeal solid tumors with or without cisplatin intervention. MATERIAL/METHODS: In this study, the expression of CD133 was detected in the Hep-2 cell line by flow cytometry. By applying magnetic cell sorting (MACS) technology, we reported the results of purifying CD133-positive cells from a Hep-2 cell line. Cell proliferation, colony formation, and tumor-forming ability were examined in vitro and in vivo to identify the marker of CSCs in Hep-2 cell line. RESULTS: Upon flow cytometry analysis, CD133 was expressed constantly on 40.12±1.32% in Hep-2 cell line. Cell proliferation and colony formation ability were higher in CD133-positive cells compared to CD133-negative cells, and the in vivo tumorigenesis experiment showed the same results as in vitro assay. The 2 subpopulations cells were both sensitive to DDP, among which, the effect of DPP on proliferation ability and tumor-forming ability of CD133-positive cells was obviously greater than that of CD133-negative cells. CONCLUSIONS: Above all, our study revealed that CD133-positive cells have properties of higher proliferation, colony formation, and tumorigenesis in Hep-2 cell line, indicating that CD133 could be a marker to characterize laryngeal cancer stem cells. International Scientific Literature, Inc. 2016-04-06 /pmc/articles/PMC4825878/ /pubmed/27049928 http://dx.doi.org/10.12659/MSM.895645 Text en © Med Sci Monit, 2016 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License |
spellingShingle | Molecular Biology Qiu, Hai-ou Wang, Huifang Che, Na Li, Dong Mao, Yong Zeng, Qiao Ge, Rongming Identification and Characterization of CD133(pos) Subpopulation Cells From a Human Laryngeal Cancer Cell Line |
title | Identification and Characterization of CD133(pos) Subpopulation Cells From a Human Laryngeal Cancer Cell Line |
title_full | Identification and Characterization of CD133(pos) Subpopulation Cells From a Human Laryngeal Cancer Cell Line |
title_fullStr | Identification and Characterization of CD133(pos) Subpopulation Cells From a Human Laryngeal Cancer Cell Line |
title_full_unstemmed | Identification and Characterization of CD133(pos) Subpopulation Cells From a Human Laryngeal Cancer Cell Line |
title_short | Identification and Characterization of CD133(pos) Subpopulation Cells From a Human Laryngeal Cancer Cell Line |
title_sort | identification and characterization of cd133(pos) subpopulation cells from a human laryngeal cancer cell line |
topic | Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4825878/ https://www.ncbi.nlm.nih.gov/pubmed/27049928 http://dx.doi.org/10.12659/MSM.895645 |
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