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Comprehensive Transcriptome Meta-analysis to Characterize Host Immune Responses in Helminth Infections

Helminth infections affect more than a third of the world’s population. Despite very broad phylogenetic differences among helminth parasite species, a systemic Th2 host immune response is typically associated with long-term helminth infections, also known as the “helminth effect”. Many investigation...

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Detalles Bibliográficos
Autores principales: Zhou, Guangyan, Stevenson, Mary M., Geary, Timothy G., Xia, Jianguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4826001/
https://www.ncbi.nlm.nih.gov/pubmed/27058578
http://dx.doi.org/10.1371/journal.pntd.0004624
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author Zhou, Guangyan
Stevenson, Mary M.
Geary, Timothy G.
Xia, Jianguo
author_facet Zhou, Guangyan
Stevenson, Mary M.
Geary, Timothy G.
Xia, Jianguo
author_sort Zhou, Guangyan
collection PubMed
description Helminth infections affect more than a third of the world’s population. Despite very broad phylogenetic differences among helminth parasite species, a systemic Th2 host immune response is typically associated with long-term helminth infections, also known as the “helminth effect”. Many investigations have been carried out to study host gene expression profiles during helminth infections. The objective of this study is to determine if there is a common transcriptomic signature characteristic of the helminth effect across multiple helminth species and tissue types. To this end, we performed a comprehensive meta-analysis of publicly available gene expression datasets. After data processing and adjusting for study-specific effects, we identified ~700 differentially expressed genes that are changed consistently during helminth infections. Functional enrichment analyses indicate that upregulated genes are predominantly involved in various immune functions, including immunomodulation, immune signaling, inflammation, pathogen recognition and antigen presentation. Down-regulated genes are mainly involved in metabolic process, with only a few of them are involved in immune regulation. This common immune gene signature confirms previous observations and indicates that the helminth effect is robust across different parasite species as well as host tissue types. To the best of our knowledge, this study is the first comprehensive meta-analysis of host transcriptome profiles during helminth infections.
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spelling pubmed-48260012016-04-22 Comprehensive Transcriptome Meta-analysis to Characterize Host Immune Responses in Helminth Infections Zhou, Guangyan Stevenson, Mary M. Geary, Timothy G. Xia, Jianguo PLoS Negl Trop Dis Research Article Helminth infections affect more than a third of the world’s population. Despite very broad phylogenetic differences among helminth parasite species, a systemic Th2 host immune response is typically associated with long-term helminth infections, also known as the “helminth effect”. Many investigations have been carried out to study host gene expression profiles during helminth infections. The objective of this study is to determine if there is a common transcriptomic signature characteristic of the helminth effect across multiple helminth species and tissue types. To this end, we performed a comprehensive meta-analysis of publicly available gene expression datasets. After data processing and adjusting for study-specific effects, we identified ~700 differentially expressed genes that are changed consistently during helminth infections. Functional enrichment analyses indicate that upregulated genes are predominantly involved in various immune functions, including immunomodulation, immune signaling, inflammation, pathogen recognition and antigen presentation. Down-regulated genes are mainly involved in metabolic process, with only a few of them are involved in immune regulation. This common immune gene signature confirms previous observations and indicates that the helminth effect is robust across different parasite species as well as host tissue types. To the best of our knowledge, this study is the first comprehensive meta-analysis of host transcriptome profiles during helminth infections. Public Library of Science 2016-04-08 /pmc/articles/PMC4826001/ /pubmed/27058578 http://dx.doi.org/10.1371/journal.pntd.0004624 Text en © 2016 Zhou et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhou, Guangyan
Stevenson, Mary M.
Geary, Timothy G.
Xia, Jianguo
Comprehensive Transcriptome Meta-analysis to Characterize Host Immune Responses in Helminth Infections
title Comprehensive Transcriptome Meta-analysis to Characterize Host Immune Responses in Helminth Infections
title_full Comprehensive Transcriptome Meta-analysis to Characterize Host Immune Responses in Helminth Infections
title_fullStr Comprehensive Transcriptome Meta-analysis to Characterize Host Immune Responses in Helminth Infections
title_full_unstemmed Comprehensive Transcriptome Meta-analysis to Characterize Host Immune Responses in Helminth Infections
title_short Comprehensive Transcriptome Meta-analysis to Characterize Host Immune Responses in Helminth Infections
title_sort comprehensive transcriptome meta-analysis to characterize host immune responses in helminth infections
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4826001/
https://www.ncbi.nlm.nih.gov/pubmed/27058578
http://dx.doi.org/10.1371/journal.pntd.0004624
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