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ISOLATION AND GENOTYPING OF Toxoplasma gondii IN SERONEGATIVE URBAN RATS AND PRESENCE OF ANTIBODIES IN COMMUNICATING DOGS IN BRAZIL

The role of rodents in the epidemiology of toxoplasmosis was investigated in Londrina, Paraná State, Brazil. One hundred and eighty-one Rattus rattus and one Mus musculus were caught in 37 places. Blood and tissues were collected and submitted to the indirect fluorescence antibody test (IFAT) and th...

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Autores principales: RUFFOLO, Bruno Bergamo, TOLEDO, Roberta dos Santos, MARTINS, Felippe Danyel Cardoso, BUGNI, Felipe Monteiro, da COSTA, Letícia, MARANA, Elizabete Regina Marangoni, NAVARRO, Italmar Teodorico, GARCIA, João Luis, SU, Chunlei, FREIRE, Roberta Lemos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4826081/
https://www.ncbi.nlm.nih.gov/pubmed/27074322
http://dx.doi.org/10.1590/S1678-9946201658028
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author RUFFOLO, Bruno Bergamo
TOLEDO, Roberta dos Santos
MARTINS, Felippe Danyel Cardoso
BUGNI, Felipe Monteiro
da COSTA, Letícia
MARANA, Elizabete Regina Marangoni
NAVARRO, Italmar Teodorico
GARCIA, João Luis
SU, Chunlei
FREIRE, Roberta Lemos
author_facet RUFFOLO, Bruno Bergamo
TOLEDO, Roberta dos Santos
MARTINS, Felippe Danyel Cardoso
BUGNI, Felipe Monteiro
da COSTA, Letícia
MARANA, Elizabete Regina Marangoni
NAVARRO, Italmar Teodorico
GARCIA, João Luis
SU, Chunlei
FREIRE, Roberta Lemos
author_sort RUFFOLO, Bruno Bergamo
collection PubMed
description The role of rodents in the epidemiology of toxoplasmosis was investigated in Londrina, Paraná State, Brazil. One hundred and eighty-one Rattus rattus and one Mus musculus were caught in 37 places. Blood and tissues were collected and submitted to the indirect fluorescence antibody test (IFAT) and the bioassay. Serum samples from 61 contacting dogs were also collected. Sixteen rats (8.8%) were positive for Toxoplasma gondii, but just two of them were positive by serology and bioassay test. Antibodies were found in nine (4.9%) rats. Tissues of nine rats bioassayed were positive and four isolates were obtained. Restriction fragment length polymorphism (RFLP) analysis was performed using 12 markers (SAG1, SAG2, SAG2-alt, C22-8, C29-2, L358, PK1, BTUB, GRA6, SAG3, Apico, CS3). Genotyping revealed that the four strains isolated from this study have been isolated before in cats and chickens from Brazil. None of the isolates was identified like clonal archetypal T-types I, II, and III. The rats presented lower serologic Toxoplasma gondii prevalence (8.8%) compared to contacting dogs (70.5%).
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spelling pubmed-48260812016-04-21 ISOLATION AND GENOTYPING OF Toxoplasma gondii IN SERONEGATIVE URBAN RATS AND PRESENCE OF ANTIBODIES IN COMMUNICATING DOGS IN BRAZIL RUFFOLO, Bruno Bergamo TOLEDO, Roberta dos Santos MARTINS, Felippe Danyel Cardoso BUGNI, Felipe Monteiro da COSTA, Letícia MARANA, Elizabete Regina Marangoni NAVARRO, Italmar Teodorico GARCIA, João Luis SU, Chunlei FREIRE, Roberta Lemos Rev Inst Med Trop Sao Paulo Original Article The role of rodents in the epidemiology of toxoplasmosis was investigated in Londrina, Paraná State, Brazil. One hundred and eighty-one Rattus rattus and one Mus musculus were caught in 37 places. Blood and tissues were collected and submitted to the indirect fluorescence antibody test (IFAT) and the bioassay. Serum samples from 61 contacting dogs were also collected. Sixteen rats (8.8%) were positive for Toxoplasma gondii, but just two of them were positive by serology and bioassay test. Antibodies were found in nine (4.9%) rats. Tissues of nine rats bioassayed were positive and four isolates were obtained. Restriction fragment length polymorphism (RFLP) analysis was performed using 12 markers (SAG1, SAG2, SAG2-alt, C22-8, C29-2, L358, PK1, BTUB, GRA6, SAG3, Apico, CS3). Genotyping revealed that the four strains isolated from this study have been isolated before in cats and chickens from Brazil. None of the isolates was identified like clonal archetypal T-types I, II, and III. The rats presented lower serologic Toxoplasma gondii prevalence (8.8%) compared to contacting dogs (70.5%). Instituto de Medicina Tropical 2016-04-08 /pmc/articles/PMC4826081/ /pubmed/27074322 http://dx.doi.org/10.1590/S1678-9946201658028 Text en http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Original Article
RUFFOLO, Bruno Bergamo
TOLEDO, Roberta dos Santos
MARTINS, Felippe Danyel Cardoso
BUGNI, Felipe Monteiro
da COSTA, Letícia
MARANA, Elizabete Regina Marangoni
NAVARRO, Italmar Teodorico
GARCIA, João Luis
SU, Chunlei
FREIRE, Roberta Lemos
ISOLATION AND GENOTYPING OF Toxoplasma gondii IN SERONEGATIVE URBAN RATS AND PRESENCE OF ANTIBODIES IN COMMUNICATING DOGS IN BRAZIL
title ISOLATION AND GENOTYPING OF Toxoplasma gondii IN SERONEGATIVE URBAN RATS AND PRESENCE OF ANTIBODIES IN COMMUNICATING DOGS IN BRAZIL
title_full ISOLATION AND GENOTYPING OF Toxoplasma gondii IN SERONEGATIVE URBAN RATS AND PRESENCE OF ANTIBODIES IN COMMUNICATING DOGS IN BRAZIL
title_fullStr ISOLATION AND GENOTYPING OF Toxoplasma gondii IN SERONEGATIVE URBAN RATS AND PRESENCE OF ANTIBODIES IN COMMUNICATING DOGS IN BRAZIL
title_full_unstemmed ISOLATION AND GENOTYPING OF Toxoplasma gondii IN SERONEGATIVE URBAN RATS AND PRESENCE OF ANTIBODIES IN COMMUNICATING DOGS IN BRAZIL
title_short ISOLATION AND GENOTYPING OF Toxoplasma gondii IN SERONEGATIVE URBAN RATS AND PRESENCE OF ANTIBODIES IN COMMUNICATING DOGS IN BRAZIL
title_sort isolation and genotyping of toxoplasma gondii in seronegative urban rats and presence of antibodies in communicating dogs in brazil
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4826081/
https://www.ncbi.nlm.nih.gov/pubmed/27074322
http://dx.doi.org/10.1590/S1678-9946201658028
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